Cat. No.: HY-17403

Manidipine dihydrochloride

(Synonyms: CV-4093)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Manidipine dihydrochloride is a third-generation, lipophilic, orally active and highly vasoselective calcium channel antagonist (IC₅₀ = 2.6 nM in guinea-pig ventricular cells) and acts as an antihypertensive agent. Manidipine effectively reduces blood pressure as well as improving insulin sensitivity, renal protection, and antiatherosclerotic activity. Manidipine also exerts anti-inflammatory activity mediated by NF-κB and antiviral activity against many flavivirus and negative-strand RNA viruses through the inhibition of calcium channel. Manidipine is widely applied to research of cardiovascular, metabolic disease and infection.

For research use only. We do not sell to patients.

Purity: 99.84%
CAS No.: 89226-75-5
Formula: C₃₅H₄₀Cl₂N₄O₆
Molecular Weight:683.62

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Manidipine dihydrochloride Chemical Structure

CAS No.: 89226-75-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

T-type calcium channel

All Calcium Channel Isoforms :

All NF-κB Isoforms :

In Vitro

Manidipine dihydrochloride (1 μM, 42 h) suppresses IL-6 secretion in lipoproteins-induced HUVEC^[3].
Manidipine dihydrochloride (1 μM, 16 h) suppresses IL-6 secretion in 10 ng/mL IL-1, 10 ng/mL IFN-γ, and 25 ng/mL TNFα treated THP-I ^[3].
Manidipine dihydrochloride (20 mg/mL, 48/20 h) inhibits propagation/genome replication of SFTSV (a negative-strand RNA virus) in SW13 cells^[4].
Manidipine dihydrochloride (20 mg/mL, 48 h) interferes SFTSV N-induced inclusion body formation in Huh-7 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Manidipine dihydrochloride (10 mg/kg, i.p., b.i.d. at day 4, day 5) prolongs survival in SFTSV-infected mice^[4].
Manidipine dihydrochloride (25 mg/kg, i.p., b.i.d. for 2 d, then daily for 19 d) protects mice from JEV infection^[5].
Manidipine dihydrochloride (3 mg/kg, i.g., once per day, 7 d) prevents isoproterenol-induced left ventricular hypertrophy in rats^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

IFNAR^-/- mice^[4]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p. ), b.i.d. at day 4, day 5
Result:

Exhibited a modest, but statistically significant increase in the survival rate of lethal animal model with SFTSV infection, significantly reduced viral titers in the spleen and kidney.

Animal Model:

Adult female BALB/c mice (age, 4 weeks) ^[5]
Dosage:

25 mg/kg
Administration:

Intraperitoneal injection (i.p. ), b.i.d. for 2 days, then daily for 19 days
Result:

Reduced the mortality rate from 73% to 20%, significantly reduced the viral load in infected mice while remarkably alleviated brain damage phenomena. Had little effect on peripheral JEV infection, which indicated that manidipine protected the mice against JEV-induced lethality by decreasing the viral load in the brain.

Animal Model:

8-week-old male Wistar rats^[7]
Dosage:

3 mg/kg
Administration:

Intragastric gavage (i.g.), once per day for 7 d
Result:

Prevented isoproterenol-induced left ventricular hypertrophy (2.26±0.02 g/kg; p<0.01) as isoproterenol increased left ventricular weight (2.40±0.04 g/kg; p<0.01). Inhibited expression of mRNA of ANP (0.9-fold of the control value; p<0.01), collagen types I (1.1-fold; p<0.01) and type III (1.6-fold; p<0.01), and fibronectin (1.1-fold; p<0.01).

Chemical Information

CAS No. 89226-75-5
Appearance Solid
Molecular Weight 683.62
Formula C₃₅H₄₀Cl₂N₄O₆
Color White to off-white
SMILES

O=C(C1=C(C)NC(C)=C(C(OC)=O)C1C2=CC=CC([N+]([O-])=O)=C2)OCCN3CCN(C(C4=CC=CC=C4)C5=CC=CC=C5)CC3.[H]Cl.[H]Cl
Synonyms

CV-4093
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (3)

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Journal Impact Factor

Int Immunopharmacol

Regulation of P-glycoprotein through CaMKII/cPLA2 pathway in lymphocytes for treating refractory rheumatoid arthritis by manidipine. [Abstract]

2025 May 27:156:114735. PMID: 40294472
Biomed Pharmacother

Docking and database screening identify manidipine as a potential modulator of matrix metalloproteinase-7 in chronic kidney disease. [Abstract]

2025 Dec:193:118748. PMID: 41232355
bioRxiv

Drug-induced phospholipidosis as an artifact in antiviral drug repurposing. [Abstract]

2025 Nov 21:2025.11.20.689520. PMID: 41332603

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (73.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4628 mL

7.3140 mL

14.6280 mL

5 mM

0.2926 mL

1.4628 mL

2.9256 mL

10 mM

0.1463 mL

0.7314 mL

1.4628 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Roca-Cusachs A, et al. Antihypertensive effect of manidipine. Drugs. Drugs. 2005，65，11-19. [Content Brief]

[2]. Cavalieri L,, et al. Metabolic effects of manidipine. Am J Cardiovasc Drugs. 2009，9，3. [Content Brief]

[3]. Franco Bernini, et al. Manidipine reduces pro-inflammatory cytokines secretion in human endothelial cells and macrophages. Pharmacol Res. 2010，62，3. [Content Brief]

[4]. Urata S, et al. Calcium Influx Regulates the Replication of Several Negative-Strand RNA Viruses Including Severe Fever with Thrombocytopenia Syndrome Virus. J Virol. 2023，97，3. [Content Brief]

[5]. Wang S, et al. Screening of FDA-Approved Drugs for Inhibitors of Japanese Encephalitis Virus Infection. J Virol. 2017，91，21. [Content Brief]

[6]. Tohse N, et al. Voltage-dependent modulation of L-type Ca2+ current by manidipine in guinea-pig heart cells. Eur J Pharmacol. 1993，249(2):231-3. [Content Brief]

[7]. Yoshiyama M, et al. Effect of manidipine hydrochloride, a calcium antagonist, on isoproterenol-induced left ventricular hypertrophy. Pharmacol Res. 2010Jpn Circ J. 1998，62(1):47-52. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4628 mL	7.3140 mL	14.6280 mL	36.5700 mL
	5 mM	0.2926 mL	1.4628 mL	2.9256 mL	7.3140 mL
	10 mM	0.1463 mL	0.7314 mL	1.4628 mL	3.6570 mL
	15 mM	0.0975 mL	0.4876 mL	0.9752 mL	2.4380 mL
	20 mM	0.0731 mL	0.3657 mL	0.7314 mL	1.8285 mL
	25 mM	0.0585 mL	0.2926 mL	0.5851 mL	1.4628 mL
	30 mM	0.0488 mL	0.2438 mL	0.4876 mL	1.2190 mL
	40 mM	0.0366 mL	0.1829 mL	0.3657 mL	0.9143 mL
	50 mM	0.0293 mL	0.1463 mL	0.2926 mL	0.7314 mL
	60 mM	0.0244 mL	0.1219 mL	0.2438 mL	0.6095 mL

Manidipine dihydrochloride

3 Cited Publications

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Manidipine dihydrochloride

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Publications Citing Use of MedChemExpress (MCE) Manidipine dihydrochloride

All Calcium Channel Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Manidipine dihydrochloride Related Classifications

HY-17403 Related Classifications