NS8593

NS8593 reversibly suppresses SK3 current; IC₅₀ is 91 nM in HEK293 cells^[1].
NS8593 (10 μM, 4 min) has no significant effect on human IK (hIK) current in HEK293 cells^[1].
NS8593's (3 μM) inhibitory effect on HEK293 cells decreased with increasing intracellular Ca²⁺ concentration (K_d = 0.47 μM at 0.3 μM Ca²⁺ and K_d = 14 μM at 10 μM Ca²⁺)^[1].
NS8593 (3 μM, 4 min) shifts the Ca²⁺ activation curve to the right (EC₅₀ increases from 0.43 μM to 1.6 μM) and reduces the Hill coefficient (from 5.6 to 2.0) in HEK293 cells^[1].
NS8593 cannot displace the binding of lapamin at a concentration of 10 μM, while lapamin, UCL 1684 and dequalinium completely inhibit the binding in HEK293 cells^[1].
NS8593 (1 μM, 30 μM) reversibly suppresses TRPM7 current in HEK 293 cells; 1 μM NS8593 partially suppresses it, and 30 μM almost completely suppresses it; in solution without Mg²⁺, the IC₅₀ is 1.6 μM; in the presence of 300 μM Mg²⁺, the IC₅₀ increases to 5.9 μM^[2].
NS8593 (10-100 μM) inhibits TRPM3 current in HEK293 cells (IC₅₀ = 26.7 μM), but is specific for TRPM7 at 10 μM and does not affect TRPM8, TRPM2, TRPM5, TRPC6, TRPV1 or TRPA1^[2].
NS8593 (10-30 μM, 24-48 h), following long-term exposure, inhibits TRPM7-dependent changes in HEK293 cell motility and morphology, confirming the role of TRPM7 in cell migration^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.