2. Membrane Transporter/Ion Channel Neuronal Signaling Metabolic Enzyme/Protease
  3. GlyT Endogenous Metabolite
  4. N-Arachidonylglycine

N-Arachidonylglycine  (Synonyms: NA-Gly)

Cat. No.: HY-103332 Purity: 98.82%
COA Handling Instructions

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N-Arachidonylglycine (NA-Gly), a carboxylic analog of the endocannabinoid anandamide (AEA), is a GPR18 agonist (EC50 = 44.5 nM). Unlike AEA, N-Arachidonylglycine has no activity at either CB1 or CB2 receptors. N-Arachidonylglycine inhibits GLYT2 (IC50 = 5.1 μM). N-Arachidonylglycine also is an effective activator of endometrial cell migration.

For research use only. We do not sell to patients.

N-Arachidonylglycine Chemical Structure

N-Arachidonylglycine Chemical Structure

CAS No. : 179113-91-8

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10 mg USD 94 In-stock
25 mg USD 209 In-stock
50 mg USD 308 In-stock
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Based on 1 publication(s) in Google Scholar

N-Arachidonylglycine Related Antibodies

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Description

N-Arachidonylglycine (NA-Gly), a carboxylic analog of the endocannabinoid anandamide (AEA), is a GPR18 agonist (EC50 = 44.5 nM). Unlike AEA, N-Arachidonylglycine has no activity at either CB1 or CB2 receptors. N-Arachidonylglycine inhibits GLYT2 (IC50 = 5.1 μM). N-Arachidonylglycine also is an effective activator of endometrial cell migration[1][2].

IC50 & Target

GlyT2

5.1 μM (IC50)

In Vitro

N-Arachidonylglycine (0.1 nM-100 µM; 5 min) drives MAPK activation in GPR18-transfected HEK293 cells[1].
N-Arachidonylglycine shows no activity at GLYT1 or GAT1 at concentrations up to 100 μm[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N-Arachidonylglycine Related Antibodies

Western Blot Analysis[1]

Cell Line: HEK293-GPR18 cells
Concentration: 0.1 nM-100 µM
Incubation Time: 5 min
Result: Drove MAPK activation.
In Vivo

N-Arachidonylglycine (10 mg/kg; oral) increases blood concentrations of anandamide 9-fold[3].
N-Arachidonylglycine (1.2 mg/kg; oral; once) results in a significant 70% reduction of peritoneal cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats[3]
Dosage: 10 mg/kg
Administration: Oral
Result: Inhibition of FAAH, causing a reduction in the hydrolytic cleavage of anandamid.
Animal Model: Mouse (peritonitis model)[3]
Dosage: 1.2 mg/kg
Administration: Oral; once
Result: Resulted in a significant 70% reduction of peritoneal cells.
Molecular Weight

361.52

Formula

C22H35NO3
CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

O=C(O)CNC(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (276.61 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (138.30 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7661 mL 13.8305 mL 27.6610 mL
5 mM 0.5532 mL 2.7661 mL 5.5322 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.82%

COA (238 KB) HNMR (263 KB) LCMS (248 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.7661 mL 13.8305 mL 27.6610 mL 69.1525 mL
5 mM 0.5532 mL 2.7661 mL 5.5322 mL 13.8305 mL
10 mM 0.2766 mL 1.3830 mL 2.7661 mL 6.9152 mL
15 mM 0.1844 mL 0.9220 mL 1.8441 mL 4.6102 mL
20 mM 0.1383 mL 0.6915 mL 1.3830 mL 3.4576 mL
25 mM 0.1106 mL 0.5532 mL 1.1064 mL 2.7661 mL
30 mM 0.0922 mL 0.4610 mL 0.9220 mL 2.3051 mL
40 mM 0.0692 mL 0.3458 mL 0.6915 mL 1.7288 mL
50 mM 0.0553 mL 0.2766 mL 0.5532 mL 1.3830 mL
60 mM 0.0461 mL 0.2305 mL 0.4610 mL 1.1525 mL
80 mM 0.0346 mL 0.1729 mL 0.3458 mL 0.8644 mL
100 mM 0.0277 mL 0.1383 mL 0.2766 mL 0.6915 mL
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N-Arachidonylglycine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Arachidonylglycine179113-91-8NA-GlyGlyTEndogenous MetaboliteGlycine transportersendocannabinoidanandamideAEAGLYT2endometrialInhibitorinhibitorinhibit

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