N-Methyltyramine hydrochloride

Cat. No.: HY-W153159 Purity: 99.84%: Data Sheet
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N-Methyltyramine hydrochloride is an orally active α2-adrenoreceptor antagonist with an IC₅₀ value of 5.53 μM against rat targets. N-Methyltyramine hydrochloride blocks α2-adrenoreceptors, while inhibiting lipolysis, hyperactivity responses and small intestinal peristalsis in mice. N-Methyltyramine hydrochloride promotes gastrin release and pancreatic juice secretion, upregulates appetite, blood pressure, myocardial contraction frequency and contraction intensity, and increases renal blood flow, renal vascular resistance and mean peripheral arterial resistance. N-Methyltyramine hydrochloride relaxes mouse small intestinal smooth muscle and undergoes biotransformation in vivo to produce adrenaline. N-Methyltyramine hydrochloride can be used in studies related to gastrointestinal diseases.

For research use only. We do not sell to patients.

N-Methyltyramine hydrochloride Chemical Structure

CAS No. : 13062-76-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Other Forms of N-Methyltyramine hydrochloride:

N-Methyltyramine In-stock
N-Methyltyramine hydrochloride (Standard) Get quote

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α adrenergic receptor β adrenergic receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

α2-adrenergic receptor

5.53 μM (IC₅₀)

In Vitro

N-Methyltyramine hydrochloride mildly inhibits lipolysis in isolated rat and human adipocytes in vitro^[1].
N-Methyltyramine hydrochloride inhibits the binding of [³H]-para-amino Clonidine (HY-12721) to α₂-adrenergic receptors in crude synaptosomal membranes from rat brain, with an IC₅₀ of 5.53 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

N-Methyltyramine hydrochloride maximally stimulates gastrin release in rats at a dose of 25 μg/kg, with an ED₅₀ of approximately 10 μg/mL^[1].
N-Methyltyramine (infused into stomach) hydrochloride dose-dependently stimulates pancreatic secretion in rats when infused into the stomach, with beer-derived NMT capable of driving greater than 50% secretion^[1].
N-Methyltyramine hydrochloride administered intravenously at 0.04 mg/kg/min increases renal hemodynamic parameters and mean arterial pressure in dogs, while a 0.25 mg/kg i.v. bolus markedly elevates peripheral resistance^[1].
Radio-labeled N-Methyltyramine (i.v.; single dose) hydrochloride distributes rapidly to all organs (with highest levels in liver and kidneys) and is rapidly excreted and metabolized in rabbits and mice following intravenous administration^[1].
N-methyltyramine (20-100 mg/kg; i.p.; single dose) hydrochloride acts as an α₂-adrenoceptor antagonist, dose-dependently inhibiting scopolamine (HY-N0296)-induced hypermotility in male ddY mice^[2].
N-Methyltyramine (3-30 mg/kg; p.o.; single dose) hydrochloride dose-dependently inhibits mouse small intestinal propulsive function, with significant effects observed at 3 mg/kg, 9 mg/kg, and 30 mg/kg via oral administration^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

187.67

Formula

C₉H₁₄ClNO

CAS No.

13062-76-5

Appearance

Solid

Color

White to off-white

SMILES

CNCCC1=CC=C(O)C=C1.Cl

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (532.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (133.21 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3285 mL	26.6425 mL	53.2850 mL
5 mM	1.0657 mL	5.3285 mL	10.6570 mL
10 mM	0.5329 mL	2.6643 mL	5.3285 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.84%

Select Batch:

Data Sheet (273 KB) SDS (394 KB)

COA (245 KB) HNMR (239 KB) RP-HPLC (275 KB) LCMS (327 KB)

Handling Instructions (2659 KB)

References

[1]. Stohs SJ. A Review of the Receptor Binding and Pharmacological Effects of N-methyltyramine. Phytotherapy Research. 2014;29(1):14-16. [Content Brief]

[2]. Koda H, et al. Antagonistic Effect of N-Methyltyramine on α₂-Adrenoceptor in Mice. Jpn J Pharmacol. 1999;81:313-315.

[3]. Ni J, et al. Effect of N-methyltyramine on the regulation of adrenergic receptors via enzymatic epinephrine synthesis for the treatment of gastrointestinal disorders. Biomedicine & Pharmacotherapy. 2019;111:1393-1398. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.3285 mL	26.6425 mL	53.2850 mL	133.2126 mL
	5 mM	1.0657 mL	5.3285 mL	10.6570 mL	26.6425 mL
	10 mM	0.5329 mL	2.6643 mL	5.3285 mL	13.3213 mL
	15 mM	0.3552 mL	1.7762 mL	3.5523 mL	8.8808 mL
	20 mM	0.2664 mL	1.3321 mL	2.6643 mL	6.6606 mL
	25 mM	0.2131 mL	1.0657 mL	2.1314 mL	5.3285 mL
	30 mM	0.1776 mL	0.8881 mL	1.7762 mL	4.4404 mL
	40 mM	0.1332 mL	0.6661 mL	1.3321 mL	3.3303 mL
	50 mM	0.1066 mL	0.5329 mL	1.0657 mL	2.6643 mL
	60 mM	0.0888 mL	0.4440 mL	0.8881 mL	2.2202 mL
	80 mM	0.0666 mL	0.3330 mL	0.6661 mL	1.6652 mL
	100 mM	0.0533 mL	0.2664 mL	0.5329 mL	1.3321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.