1. NF-κB
  2. IKK
  3. NF-κB-IN-1

NF-κB-IN-1 

Cat. No.: HY-138537 Purity: 99.84%
Handling Instructions

NF-κB-IN-1, a 4-arylidene crucumin analogue, is a potent NF-κB signaling pathway inhibitor. NF-κB-IN-1 directly inhibits IKK to block NF-κB activation. NF-κB-IN-1 effectively inhibits the viability of lung cancer cells and attenuates the clonogenic activity of A549 cells.

For research use only. We do not sell to patients.

NF-κB-IN-1 Chemical Structure

NF-κB-IN-1 Chemical Structure

CAS No. : 1227098-15-8

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 560 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 560 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 480 In-stock
Estimated Time of Arrival: December 31
10 mg USD 800 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1550 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2400 In-stock
Estimated Time of Arrival: December 31
100 mg USD 3600 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Description

NF-κB-IN-1, a 4-arylidene crucumin analogue, is a potent NF-κB signaling pathway inhibitor. NF-κB-IN-1 directly inhibits IKK to block NF-κB activation. NF-κB-IN-1 effectively inhibits the viability of lung cancer cells and attenuates the clonogenic activity of A549 cells[1].

IC50 & Target

IKK[1]

In Vitro

NF-κB-IN-1 (compound 17) (0.001-100 μM; 72 h) inhibits the growth of A549, H1944, H460 and H157 cells, with GI50s of 0.72, 0.07, 0.13, and 0.16 μM, respectively[1].
NF-κB-IN-1 (0.5-25 μM; pretreated for 30 min or 4 h) potently blocks the IκB phosphorylation and degradation in A549 cells[1].
NF-κB-IN-1 (0.1-100 μM; pretreated for 30 min) dose-dependently inhibits the TNFα-induced nuclear translocation of NF-κB in A549 cells, with an IC50s of 1.0 μM[1].
NF-κB-IN-1 (0.1-0.4 μM; 9 d) inhibits lung cancer clonogenic activity[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A549, H1944, H460 and H157 cells
Concentration: 0.001, 0.01, 0.1, 1, 10, 100 μM
Incubation Time: 72 hours
Result: Decreased viability of lung adenocarcinoma cells A549 and H1944, quamous cell carcinoma cells H157, and large cell carcinoma cells H460 in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 0.5, 1.0, 2.5, 5.0, 10.0, 25.0 μM
Incubation Time: Pretreated for 30 min or 4 h
Result: Blocked IκB phosphorylation, with an IC50 of 2.8 μM.
Blocked IκB degradation in a dose-dependent manner.
Molecular Weight

530.57

Formula

C₃₁H₃₀O₈

CAS No.
SMILES

O=C(/C=C/C(C=C1)=CC(OC)=C1OC)/C(C(/C=C/C(C=C2)=CC(OC)=C2OC)=O)=C/C(C=C3)=CC(OC)=C3O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (94.24 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8848 mL 9.4238 mL 18.8477 mL
5 mM 0.3770 mL 1.8848 mL 3.7695 mL
10 mM 0.1885 mL 0.9424 mL 1.8848 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.71 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

NF-κB-IN-1IKKIκB kinaseI kappa B kinase4-arylidenecrucuminNF-κBsignalingpathwaycancerA549Inhibitorinhibitorinhibit

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Product Name:
NF-κB-IN-1
Cat. No.:
HY-138537
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