NF-κB-IN-1

Name: NF-κB-IN-1
Brand: MedChemExpress
SKU: HY-138537
Rating: 5.0 (3 reviews)

Cat. No.: HY-138537 Purity: 98.37%: Data Sheet
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NF-κB-IN-1, a 4-arylidene crucumin analogue, is a potent NF-κB signaling pathway inhibitor. NF-κB-IN-1 directly inhibits IKK to block NF-κB activation. NF-κB-IN-1 effectively inhibits the viability of lung cancer cells and attenuates the clonogenic activity of A549 cells.

For research use only. We do not sell to patients.

CAS No. : 1227098-15-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE NF-κB-IN-1

Flow Cytometry

Cell Imaging/Staining

: NF-κB-IN-1 purchased from MedChemExpress. Usage Cited in: Adv Biol (Weinh). 2025 Jun 4:e00761. [Abstract]; Macrophages were pretreated with inhibitors of the ERK (HY‐112287; ERK1/2 inhibitor 1), JNK (HY‐12041; SP600125), p38 MAPK (HY‐12839; p38 MAPK-IN-1), and p65 NF‐kB (HY‐138537; NF-κB-IN-1) pathways prior to incubation with pyroptotic‐CM for 4 h.The MET formation by macrophages was evaluated with SYTOX Green staining and detected by flow cytometry.

: NF-κB-IN-1 purchased from MedChemExpress. Usage Cited in: Adv Biol (Weinh). 2025 Jun 4:e00761. [Abstract]; Macrophages were pretreated with inhibitors of the ERK (HY‐112287; ERK1/2 inhibitor 1), JNK (HY‐12041; SP600125), p38 MAPK (HY‐12839; p38 MAPK-IN-1), and p65 NF‐kB (HY‐138537; NF-κB-IN-1) pathways prior to incubation with pyroptotic‐CM for 4 h. The p‐p38 and p‐p65 levels in macrophages were measured by western blotting.

: NF-κB-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 1;21(1):86. [Abstract]; Neutrophils were pretreated with inhibitors of the ERK (HY-112287;ERK1/2 inhibitor 1), p38 MAPK (HY-12839; p38 MAPK-IN-1), and p65 NF-кB (HY-138537; NF-κB-IN-1) pathways prior to incubation with hypoxic-CM from GC cells. The protein levels of p-ERK, p-p38, and p-p65 in neutrophils were measured by western blotting.

: NF-κB-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 1;21(1):86. [Abstract]; Neutrophils were pretreated with inhibitors of the ERK (HY-112287;ERK1/2 inhibitor 1), p38 MAPK (HY-12839; p38 MAPK-IN-1), and p65 NF-кB (HY-138537; NF-κB-IN-1) pathways prior to incubation with hypoxic-CM from GC cells. The NET formation by neutrophils was evaluated with MPO and citH3 staining. Magnification: 20 × . Scale bars: 50 μm.

: NF-κB-IN-1 purchased from MedChemExpress. Usage Cited in: Cancer Med. 2023 Jul;12(14):15676-15690. [Abstract]; IKK‐inhibitor (HY‐138537; NF-κB-IN-1) could block NF‐kB activation and decrease the downstream proteins HLA‐A/B/C and PD‐L1.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IKK^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	0.53 μM Compound: 17	Growth inhibition of human A549 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human A549 cells after 48 hrs by sulforhodamine B assay	[PMID: 21070043]
A549	GI₅₀	0.72 μM Compound: 17	Growth inhibition of human A549 cells after 72 hrs by alamar blue method Growth inhibition of human A549 cells after 72 hrs by alamar blue method	[PMID: 21070043]
A549	IC₅₀	1 μM Compound: 17	Inhibition of TNF-alpha-induced NFkappaB nuclear translocation in human A549 cells preincubated for 30 mins before TNFalpha challenge and measured 30 post TNFalpha challenge by fluorescence method Inhibition of TNF-alpha-induced NFkappaB nuclear translocation in human A549 cells preincubated for 30 mins before TNFalpha challenge and measured 30 post TNFalpha challenge by fluorescence method	[PMID: 21070043]
A549	IC₅₀	2.8 μM Compound: 17	Inhibition of IKK in human A549 cells assessed as inhibition of TNF-alpha-induced IkappaB phosphorylation preincubated for 30 mins before TNFalpha challenge Inhibition of IKK in human A549 cells assessed as inhibition of TNF-alpha-induced IkappaB phosphorylation preincubated for 30 mins before TNFalpha challenge	[PMID: 21070043]
NCI-H157	GI₅₀	0.16 μM Compound: 17	Growth inhibition of human NCI-H157 cells after 72 hrs by alamar blue method Growth inhibition of human NCI-H157 cells after 72 hrs by alamar blue method	[PMID: 21070043]
NCI-H1944	GI₅₀	0.07 μM Compound: 17	Growth inhibition of human NCI-H1944 cells after 72 hrs by alamar blue method Growth inhibition of human NCI-H1944 cells after 72 hrs by alamar blue method	[PMID: 21070043]
NCI-H460	GI₅₀	0.13 μM Compound: 17	Growth inhibition of human H460 cells after 72 hrs by alamar blue method Growth inhibition of human H460 cells after 72 hrs by alamar blue method	[PMID: 21070043]

In Vitro

NF-κB-IN-1 (compound 17) (0.001-100 μM; 72 h) inhibits the growth of A549, H1944, H460 and H157 cells, with GI₅₀s of 0.72, 0.07, 0.13, and 0.16 μM, respectively^[1].
NF-κB-IN-1 (0.5-25 μM; pretreated for 30 min or 4 h) potently blocks the IκB phosphorylation and degradation in A549 cells^[1].
NF-κB-IN-1 (0.1-100 μM; pretreated for 30 min) dose-dependently inhibits the TNFα-induced nuclear translocation of NF-κB in A549 cells, with an IC₅₀s of 1.0 μM^[1].
NF-κB-IN-1 (0.1-0.4 μM; 9 d) inhibits lung cancer clonogenic activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A549, H1944, H460 and H157 cells
Concentration:	0.001, 0.01, 0.1, 1, 10, 100 μM
Incubation Time:	72 hours
Result:	Decreased viability of lung adenocarcinoma cells A549 and H1944, quamous cell carcinoma cells H157, and large cell carcinoma cells H460 in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	A549 cells
Concentration:	0.5, 1.0, 2.5, 5.0, 10.0, 25.0 μM
Incubation Time:	Pretreated for 30 min or 4 h
Result:	Blocked IκB phosphorylation, with an IC₅₀ of 2.8 μM. Blocked IκB degradation in a dose-dependent manner.

Molecular Weight

530.57

Formula

C₃₁H₃₀O₈

CAS No.

1227098-15-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(/C=C/C(C=C1)=CC(OC)=C1OC)/C(C(/C=C/C(C=C2)=CC(OC)=C2OC)=O)=C/C(C=C3)=CC(OC)=C3O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (94.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8848 mL	9.4238 mL	18.8477 mL
5 mM	0.3770 mL	1.8848 mL	3.7695 mL
10 mM	0.1885 mL	0.9424 mL	1.8848 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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Dosing volume
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μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.37%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (248 KB) HNMR (284 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Qiu X, et, al. Synthesis and identification of new 4-arylidene curcumin analogues as potential anticancer agents targeting nuclear factor-κB signaling pathway. J Med Chem. 2010 Dec 9;53(23):8260-73. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8848 mL	9.4238 mL	18.8477 mL	47.1191 mL
	5 mM	0.3770 mL	1.8848 mL	3.7695 mL	9.4238 mL
	10 mM	0.1885 mL	0.9424 mL	1.8848 mL	4.7119 mL
	15 mM	0.1257 mL	0.6283 mL	1.2565 mL	3.1413 mL
	20 mM	0.0942 mL	0.4712 mL	0.9424 mL	2.3560 mL
	25 mM	0.0754 mL	0.3770 mL	0.7539 mL	1.8848 mL
	30 mM	0.0628 mL	0.3141 mL	0.6283 mL	1.5706 mL
	40 mM	0.0471 mL	0.2356 mL	0.4712 mL	1.1780 mL
	50 mM	0.0377 mL	0.1885 mL	0.3770 mL	0.9424 mL
	60 mM	0.0314 mL	0.1571 mL	0.3141 mL	0.7853 mL
	80 mM	0.0236 mL	0.1178 mL	0.2356 mL	0.5890 mL

NF-κB-IN-1 Related Classifications

NF-κB
IKK

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NF-κB-IN-11227098-15-8IKKIκB kinaseI kappa B kinase4-arylidenecrucuminNF-κBsignalingpathwaycancerA549Inhibitorinhibitorinhibit

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NF-κB-IN-1

Customer Review

NF-κB-IN-1 Related Antibodies

3 Publications Citing Use of MCE NF-κB-IN-1

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Biological Activity

Purity & Documentation

References

Customer Review

NF-κB-IN-1 Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

NF-κB-IN-1 Related Classifications

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