1. Metabolic Enzyme/Protease
    Autophagy
  2. Mitochondrial Metabolism
    Autophagy
  3. NL-1

NL-1 

Cat. No.: HY-135231 Purity: 99.11%
Handling Instructions

NL-1 is a mitoNEET inhibitor with antileukemic effect. NL-1 inhibits REH and REH/Ara-C cells growth with IC50s of 47.35 µM and 56.26 µM, respectively. NL-1-mediated death in leukemic cells requires the activation of the autophagic pathway.

For research use only. We do not sell to patients.

NL-1 Chemical Structure

NL-1 Chemical Structure

CAS No. : 188532-26-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 83 In-stock
Estimated Time of Arrival: December 31
10 mg USD 75 In-stock
Estimated Time of Arrival: December 31
50 mg USD 260 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

NL-1 is a mitoNEET inhibitor with antileukemic effect. NL-1 inhibits REH and REH/Ara-C cells growth with IC50s of 47.35 µM and 56.26 µM, respectively. NL-1-mediated death in leukemic cells requires the activation of the autophagic pathway[1].

IC50 & Target

MitoNEET[1]

In Vitro

NL-1 (10-100 μM; 72 hours; REH, REH/Ara-C and ALL cell lines) treatment reduces the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. NL-1 inhibits SUPB15, NALM6 with IC50s of 29.48 µM, 94.26 µM, respectively. TOM1, BV173, NALM1 and JM1 all have similar IC50 values of around 60 µM for NL-1[1].
NL-1 (60 μM; 6 hours; REH, REH/Ara-C cell lines) treatment mediates autophagy, and inhibition of autophagy partially decreased NL-1-induced tumor cell death[1].
NL-1 pretreatment inhibits the chemotactic ability of both REH and REH/Ara-C cells to migrate towards multiple chemoattractants. The cells treated with NL1 shows a dose-dependent decrease in chemotaxis both in the REH and the REH/AraC cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines
Concentration: 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 100 μM
Incubation Time: 72 hours
Result: Reduced the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner.

Cell Autophagy Assay[1]

Cell Line: REH, REH/Ara-C cell lines
Concentration: 60 μM
Incubation Time: 6 hours
Result: Induced cell autophagy.
In Vivo

NL-1 (10 mg/kg; intraperitoneal injection; daily; for 5 days; female NSG mice) treatment shows antileukemic activity in an in vivo mouse ALL model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 10 female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; daily; for 5 days
Result: Showed antileukemic activity in an in vivo mouse ALL model.
Molecular Weight

335.46

Formula

C₁₈H₂₅NO₃S

CAS No.
SMILES

O=C(N1)SC(CC2=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C2)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (745.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9810 mL 14.9049 mL 29.8098 mL
5 mM 0.5962 mL 2.9810 mL 5.9620 mL
10 mM 0.2981 mL 1.4905 mL 2.9810 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.20 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.20 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.20 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

NL-1NL1NL 1Mitochondrial MetabolismAutophagyMitoNEETCISD1acutelymphoblasticleukemiaB-Cellviabilityautophagyantileukemicanti-cancerInhibitorinhibitorinhibit

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NL-1
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