1. Metabolic Enzyme/Protease
    Autophagy
  2. Mitochondrial Metabolism
    Autophagy
  3. NL-1

NL-1 

Cat. No.: HY-135231
Handling Instructions

NL-1 is a mitoNEET inhibitor with antileukemic effect. NL-1 inhibits REH and REH/Ara-C cells growth with IC50s of 47.35 µM and 56.26 µM, respectively. NL-1-mediated death in leukemic cells requires the activation of the autophagic pathway.

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NL-1 Chemical Structure

NL-1 Chemical Structure

CAS No. : 188532-26-5

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Description

NL-1 is a mitoNEET inhibitor with antileukemic effect. NL-1 inhibits REH and REH/Ara-C cells growth with IC50s of 47.35 µM and 56.26 µM, respectively. NL-1-mediated death in leukemic cells requires the activation of the autophagic pathway[1].

IC50 & Target

MitoNEET[1]

In Vitro

NL-1 (10-100 μM; 72 hours; REH, REH/Ara-C and ALL cell lines) treatment reduces the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. NL-1 inhibits SUPB15, NALM6 with IC50s of 29.48 µM, 94.26 µM, respectively. TOM1, BV173, NALM1 and JM1 all have similar IC50 values of around 60 µM for NL-1[1].
NL-1 (60 μM; 6 hours; REH, REH/Ara-C cell lines) treatment mediates autophagy, and inhibition of autophagy partially decreased NL-1-induced tumor cell death[1].
NL-1 pretreatment inhibits the chemotactic ability of both REH and REH/Ara-C cells to migrate towards multiple chemoattractants. The cells treated with NL1 shows a dose-dependent decrease in chemotaxis both in the REH and the REH/AraC cells[1].

Cell Proliferation Assay[1]

Cell Line: REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines
Concentration: 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 100 μM
Incubation Time: 72 hours
Result: Reduced the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner.

Cell Autophagy Assay[1]

Cell Line: REH, REH/Ara-C cell lines
Concentration: 60 μM
Incubation Time: 6 hours
Result: Induced cell autophagy.
In Vivo

NL-1 (10 mg/kg; intraperitoneal injection; daily; for 5 days; female NSG mice) treatment shows antileukemic activity in an in vivo mouse ALL model[1].

Animal Model: 10 female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; daily; for 5 days
Result: Showed antileukemic activity in an in vivo mouse ALL model.
Molecular Weight

335.46

Formula

C₁₈H₂₅NO₃S

CAS No.

188532-26-5

SMILES

O=C(N1)SC(CC2=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C2)C1=O

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Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

NL-1NL1NL 1Mitochondrial MetabolismAutophagyMitoNEETCISD1acutelymphoblasticleukemiaB-Cellviabilityautophagyantileukemicanti-cancerInhibitorinhibitorinhibit

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