Mefluleucine hydrochloride
Based on 9 publication(s) in Google Scholar
Mefluleucine (NV-513) hydrochloride, a leucine analog, is the first selective and orally active brain mTORC1 activator, binding to Sestrin2. Mefluleucine hydrochloride is used for antidepressant studies.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2639392-70-2
- Formula: C7H14ClF2NO2
- Molecular Weight:217.64
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Mefluleucine hydrochloride
More- Nature. 2025 Feb;638(8049):172-181. [Abstract]
- Cell Metab. 2024 Jun 18:S1550-4131(24)00189-X. [Abstract]
- Neuron. 2023 Oct 4;111(19):3028-3040.e6. [Abstract]
- J Neurosci. 2026 Feb 3:e0958252026. [Abstract]
- Mol Immunol. 2025 Nov 28:189:1-12. [Abstract]
- bioRxiv. 2025 Jul 21.
- biorxiv. 2024 May 27.
- McGill University. 2023 Jul 5.
- bioRxiv. 2023 Apr 24.
Customer Validation & Images
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IF
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WB
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RT-PCR
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IF
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Flow Cytometry
Biological Activity
mTORC1[1].
Mefluleucine (160 mg/kg, po, single dose) hydrochloride rapidly increases levels of phospho-mTOR as well as the downstream targets, phospho-p70S6K1, and phosphor-4EB-P1, in synaptosomal preparations of PFC[2].
Mefluleucine (80 mg/kg, po, daily for a total of 7 days) hydrochloride also produces antidepressant effects[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats weighing 250-260 g[2].
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Dosage:40, 80, 160 mg/kg.
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Administration:PO, single dose (160 mg/kg) or daily for a total of 7 days (40, 80 mg/kg).
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Result:Produced antidepressant effects.
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Animal Model:Male Sprague-Dawley (SD) rats weighed 250-400 g[1].
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Dosage:1 mg/kg, 5 mg/kg (Pharmacokinetic Design).
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Administration:I.V at 1 mg/kg and PO at 5 mg/kg.
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Result:Essentially 100% orally bioavailable with an elimination half-life in plasma of ~ 3 h.
Chemical Information
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CAS No. 2639392-70-2
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Appearance Solid
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Molecular Weight 217.64
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Formula C7H14ClF2NO2
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Color White to light yellow
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SMILES
N[C@@H](CC(C)(C(F)F)C)C(O)=O.Cl
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Synonyms
NV-5138 hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nature
2025 Feb;638(8049):172-181. PMID: 39743596
Mefluleucine hydrochloride purchased from MedChemExpress. Usage Cited in: Nature. 2025 Feb;638(8049):172-181. [Abstract]
Organoids from the control, patient, and MDLS groups were cultured for 70 days with or without NV-5138 (Mefluleucine: 50 μM) treatment, and then immunostained with the oRG cell markers HOPX and pS6.
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Cell Metab
Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer. [Abstract]2024 Jun 18:S1550-4131(24)00189-X. PMID: 38897198
Mefluleucine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2024 Jun 18:S1550-4131(24)00189-X. [Abstract]
Mouse CD8+ T cells were treated with MNAM and rescued by agonist including C16 PAF (1μM, 24h), pCS (125μM, 24h), NV-5138 (Mefluleucine: 100μM, 24h), ionomycin (1μM, 24h) and PMA (50nM, 24h). Flow cytometry detected the cytotoxicity (GZMB, TNF-α and IFN-γ) of T cells.
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Neuron
Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome. [Abstract]2023 Oct 4;111(19):3028-3040.e6. PMID: 37473758 -
J Neurosci
A Time-Sensitive Plasticity Distinguishes the Rapid and Sustained Synaptic Actions of Ketamine from Its (2 R,6 R)-Hydroxynorketamine Metabolite. [Abstract]2026 Feb 3:e0958252026. PMID: 41633835 -
Mol Immunol
Catalpol enhances mTORC1/HIF-1α/PDHK1-mediated oxidative metabolism that leads to promote the differentiation of Treg cells in RA. [Abstract]2025 Nov 28:189:1-12. PMID: 41317620
Mefluleucine hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2025 Nov 28:189:1-12. [Abstract]
Western blotting was used to examine the effects of CAT on the phosphorylation levels of P70S6K and 4EBP1 and the protein expression levels of HIF-1α and PDHK1 following pretreatment with NV5138 (Mefluleucine: 150 μM; 24 h).
Mefluleucine hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2025 Nov 28:189:1-12. [Abstract]
RT-qPCR was used to examine the effect of CAT on FOXP3 mRNA expression following pretreatment with NV5138 (Mefluleucine: 150 μM; 24 h).
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Mefluleucine hydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jul 21.
Rosettes in control and SPG11 14-day-old cortical organoids, either untreated or treated with NV5138 (Mefluleucine: 100 μM), were stained with DAPI and immunostained with antibodies against phosphorylated S6.
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Solvent & Solubility
DMSO : 100 mg/mL (459.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (459.47 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sengupta S, et al. Discovery of NV-5138, the first selective Brain mTORC1 activator. Sci Rep. 2019 Mar 11;9(1):4107. [Content Brief]
[2]. Kato T, et al. Sestrin modulator NV-5138 produces rapid antidepressant effects via direct mTORC1 activation. J Clin Invest. 2019 Apr 16;129(6):2542-2554. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 4.5947 mL | 22.9737 mL | 45.9474 mL | 114.8686 mL |
| 5 mM | 0.9189 mL | 4.5947 mL | 9.1895 mL | 22.9737 mL | |
| 10 mM | 0.4595 mL | 2.2974 mL | 4.5947 mL | 11.4869 mL | |
| 15 mM | 0.3063 mL | 1.5316 mL | 3.0632 mL | 7.6579 mL | |
| 20 mM | 0.2297 mL | 1.1487 mL | 2.2974 mL | 5.7434 mL | |
| 25 mM | 0.1838 mL | 0.9189 mL | 1.8379 mL | 4.5947 mL | |
| 30 mM | 0.1532 mL | 0.7658 mL | 1.5316 mL | 3.8290 mL | |
| 40 mM | 0.1149 mL | 0.5743 mL | 1.1487 mL | 2.8717 mL | |
| 50 mM | 0.0919 mL | 0.4595 mL | 0.9189 mL | 2.2974 mL | |
| 60 mM | 0.0766 mL | 0.3829 mL | 0.7658 mL | 1.9145 mL | |
| 80 mM | 0.0574 mL | 0.2872 mL | 0.5743 mL | 1.4359 mL | |
| 100 mM | 0.0459 mL | 0.2297 mL | 0.4595 mL | 1.1487 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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