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Cat. No.: HY-179582

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PROTAC DNPH1 Degrader 1

PROTAC DNPH1 Degrader 1 is a potent PROTAC DNPH1 degrader with a DC₅₀ of 28 nM. PROTAC DNPH1 Degrader 1 mediates proteasome- and VHL-dependent DNPH1 knockdown, drives DNPH1 ubiquitination and proteasomal degradation. PROTAC DNPH1 Degrader 1 can be used for the research of brca1 mutant cancer.
(Pink: DNPH1 ligand (HY-179303); Blue: VHL ligand (HY-150803); Black: linker).

For research use only. We do not sell to patients.

Formula: C₆₀H₆₄F₃N₁₁O₆S
Molecular Weight:1124.28

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

PROTAC DNPH1 Degrader 1 Chemical Structure

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

PROTAC DNPH1 Degrader 1 is a potent PROTAC DNPH1 degrader with a DC₅₀ of 28 nM. PROTAC DNPH1 Degrader 1 mediates proteasome- and VHL-dependent DNPH1 knockdown, drives DNPH1 ubiquitination and proteasomal degradation. PROTAC DNPH1 Degrader 1 can be used for the research of brca1 mutant cancer^[1]. (Pink: DNPH1 ligand (HY-179303); Blue: VHL ligand (HY-150803); Black: linker).

IC₅₀ & Target

VHL	DNPH1 28 nM (DC₅₀)

All PROTACs Isoforms :

In Vitro

PROTAC DNPH1 Degrader 1 (compound 59) (0-500 nM; 18-168 h) potently and completely degrades DNPH1 in SUM149PT cells via a proteasome-dependent, VHL-recruiting mechanism^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

SUM149PT cells, HiBit-tagged SUM149PT cells
Concentration:

0; 10; 50; 100; 300; 500 nM
Incubation Time:

18; 24; 48; 72; 120; 168 h
Result:

Achieved near-complete DNPH1 degradation with a DC₅₀ of 28 nM and a Dmax of 100%.
Blocked degradation by cotreatment with proteasome inhibitors and neddylation inhibitor.
Inhibited degradation by competition with DNPH1 ligand 44 and VHL ligand VHL032.
Observed near-complete DNPH1 degradation via Western blot at concentrations as low as 20 nM in parental SUM149PT cells.

Chemical Information

Molecular Weight 1124.28
Formula C₆₀H₆₄F₃N₁₁O₆S
SMILES

CNC1=NC(NC2=C(C=C(C=C2)C(O)=O)C3=CC=CC(C(F)(F)F)=C3)=NC4=CC=C(C=C41)OC5=CC=C(C=C5)CN(CCCCCC6=CN([C@H](C(N7C[C@H](O)C[C@H]7C(N[C@@H](C)C8=CC=C(C9=C(C)N=CS9)C=C8)=O)=O)C(C)C)N=N6)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Anderson NA, et al. Use of Direct-to-Biology Strategies for the Discovery of 2'-Deoxynucleoside 5'-Monophosphate N-Glycosidase (DNPH1) PROTACs. J Med Chem. 2025;68(21):23421-23447. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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PROTAC DNPH1 Degrader 1

All PROTACs Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

PROTAC DNPH1 Degrader 1 Related Classifications

HY-179582 Related Classifications