RO7185876

Name: RO7185876
Brand: MedChemExpress
SKU: HY-145343
Rating: 4.5 (0 reviews)

Cat. No.: HY-145343 Pureza: 99.9%: Ficha de datos
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RO7185876 is a potent and selective gamma secretase modulator as a potential research for Alzheimer's disease.

Para uso exclusivo en investigación. No vendemos a pacientes.

RO7185876 Estructura química

No. CAS : 2226038-71-5

Tamaño	Stock	Cantidad
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	Obtener un presupuesto
50 mg	Obtener un presupuesto

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Based on 1 Customer Validation

Other Forms of RO7185876:

(9R)-RO7185876 In-stock

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Descripciòn

RO7185876 is a potent and selective gamma secretase modulator as a potential research for Alzheimer's disease.

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	2 nM Compound: (S)-3; RO7185876	Inhibition of human Notch1 expressed in HEK293 cells coexpressing luciferase reporter measured after 20 hrs by chemiluminescence assay Inhibition of human Notch1 expressed in HEK293 cells coexpressing luciferase reporter measured after 20 hrs by chemiluminescence assay	[PMID: 32551009]
HEK293	IC₅₀	2 nM Compound: (S)-3; RO7185876	Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in total amyloid beta (1 to 42 residues) production Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in total amyloid beta (1 to 42 residues) production	[PMID: 32551009]
HEK293	IC₅₀	4 nM Compound: (S)-3; RO7185876	Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in free amyloid beta (1 to 42 residues) production Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in free amyloid beta (1 to 42 residues) production	[PMID: 32551009]
N2a	IC₅₀	2 nM Compound: (S)-3; RO7185876	Modulation of mouse gamma secretase in N2A cells assessed as reduction in total amyloid beta (1 to 42 residues) production Modulation of mouse gamma secretase in N2A cells assessed as reduction in total amyloid beta (1 to 42 residues) production	[PMID: 32551009]
N2a	IC₅₀	4 nM Compound: (S)-3; RO7185876	Modulation of mouse gamma secretase in N2A cells assessed as reduction in free amyloid beta (1 to 42 residues) production Modulation of mouse gamma secretase in N2A cells assessed as reduction in free amyloid beta (1 to 42 residues) production	[PMID: 32551009]

Peso molecular

483.53

Fòrmula

C₂₅H₂₈F₃N₇

No. CAS

2226038-71-5

Appearance

Solid

Color

White to off-white

SMILES

FC(C(F)=C(C=C1)F)=C1[C@@]2([H])C3=NC(N[C@H]4[C@@H]5CN(C6=NC=NC(C)=C6)C[C@H]4CC5)=NN3CCCC2

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (206.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0681 mL	10.3406 mL	20.6812 mL
5 mM	0.4136 mL	2.0681 mL	4.1362 mL
10 mM	0.2068 mL	1.0341 mL	2.0681 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.9%

Select Batch:

Ficha de datos (277 KB) SDS (251 KB)

COA (257 KB) HNMR (105 KB) CNMR (88 KB) RP-HPLC (108 KB) LCMS (129 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Ratni H, et al. Discovery of RO7185876, a Highly Potent γ-Secretase Modulator (GSM) as a Potential Treatment for Alzheimer's Disease. ACS Med Chem Lett. 2020 Apr 27;11(6):1257-1268. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0681 mL	10.3406 mL	20.6812 mL	51.7031 mL
	5 mM	0.4136 mL	2.0681 mL	4.1362 mL	10.3406 mL
	10 mM	0.2068 mL	1.0341 mL	2.0681 mL	5.1703 mL
	15 mM	0.1379 mL	0.6894 mL	1.3787 mL	3.4469 mL
	20 mM	0.1034 mL	0.5170 mL	1.0341 mL	2.5852 mL
	25 mM	0.0827 mL	0.4136 mL	0.8272 mL	2.0681 mL
	30 mM	0.0689 mL	0.3447 mL	0.6894 mL	1.7234 mL
	40 mM	0.0517 mL	0.2585 mL	0.5170 mL	1.2926 mL
	50 mM	0.0414 mL	0.2068 mL	0.4136 mL	1.0341 mL
	60 mM	0.0345 mL	0.1723 mL	0.3447 mL	0.8617 mL
	80 mM	0.0259 mL	0.1293 mL	0.2585 mL	0.6463 mL
	100 mM	0.0207 mL	0.1034 mL	0.2068 mL	0.5170 mL

RO7185876 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RO71858762226038-71-5RO 7185876RO-7185876γ-secretaseGamma secretaseInhibitorinhibitorinhibit

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RO7185876

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RO7185876 Related Antibodies

Recomendaciones destacadas

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•Related Proteins:

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Referencias

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Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

RO7185876 Related Classifications

Keywords:

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