1. Immunology/Inflammation Anti-infection
  2. NO Synthase HSV
  3. S-Methylisothiourea sulfate

S-Methylisothiourea sulfate is a potent, selective and competitive inhibitor of inducible nitric oxide synthase (iNOS). S-Methylisothiourea sulfate exerts beneficial effects in rodent models of septic shock.

For research use only. We do not sell to patients.

S-Methylisothiourea sulfate Chemical Structure

S-Methylisothiourea sulfate Chemical Structure

CAS No. : 867-44-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
25 mg USD 50 In-stock
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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE S-Methylisothiourea sulfate

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

S-Methylisothiourea sulfate is a potent, selective and competitive inhibitor of inducible nitric oxide synthase (iNOS). S-Methylisothiourea sulfate exerts beneficial effects in rodent models of septic shock[1].

IC50 & Target

iNOS[1]

In Vitro

S-Methylisothiourea sulfate is a competitive inhibitor of iNOS activity at the L-arginine site[1].
S-Methylisothiourea sulfate prevents the NO-mediated cytotoxic effect of LPS in cultured macrophages[1].
S-Methylisothiourea sulfate (100 nM-100 μM) exhibits inhibitory effects on LPS (ug/mL)-induced nitrite production in J774.2 macrophages and rat aortic vascular smooth muscle cells[1].
S-Methylisothiourea sulfate (up to 1 mM) does not inhibit the activity of xanthine oxidase, diaphorase, lactate dehydrogenase, monoamine oxidase, catalase, cytochrome P450, or superoxide dismutase[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

S-Methylisothiourea sulfate (0.01-3 mg/kg; i.v.) dose-dependently reverses the hypotension and the vascular hyporeactivity to vasoconstrictor agents caused by endotoxin LPS in anesthetized rats[1].
S-Methylisothiourea sulfate (5 mg/kg; i.p.; given 2 hr after LPS; 10 mg/kg; i.p.) attenuates the rises in plasma alanine and aspartate aminotransferases, bilirubin, and creatinine and also prevents hypocalcaemia when measured 6 hr after administration of LPS[1].
S-Methylisothiourea sulfate (1 mg/kg; i.p.) improves 24-hr survival of mice treated with a high dose of LPS (60 mg/kg; i.p.)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (260-320 g)[1]
Dosage: 0.01 mg/kg, 0.1 mg/kg, 1 mg/kg, 3 mg/kg
Administration: Intravenous injection
Result: Caused a prompt restoration of the blood pressure to pre-LPS levels at 3 mg/kg dose in LPS (10 mg/kg, i.v.)-treated rats; Inhibited iNOS activity measured in homogenates of lung.
Molecular Weight

139.18

Formula

C2H6N2S.1/2H2O4S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC(SC)=N.O=S(O)(O)=O.[1/2]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 65 mg/mL (467.02 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.1849 mL 35.9247 mL 71.8494 mL
5 mM 1.4370 mL 7.1849 mL 14.3699 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (359.25 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 7.1849 mL 35.9247 mL 71.8494 mL 179.6235 mL
5 mM 1.4370 mL 7.1849 mL 14.3699 mL 35.9247 mL
10 mM 0.7185 mL 3.5925 mL 7.1849 mL 17.9624 mL
15 mM 0.4790 mL 2.3950 mL 4.7900 mL 11.9749 mL
20 mM 0.3592 mL 1.7962 mL 3.5925 mL 8.9812 mL
25 mM 0.2874 mL 1.4370 mL 2.8740 mL 7.1849 mL
30 mM 0.2395 mL 1.1975 mL 2.3950 mL 5.9875 mL
40 mM 0.1796 mL 0.8981 mL 1.7962 mL 4.4906 mL
50 mM 0.1437 mL 0.7185 mL 1.4370 mL 3.5925 mL
60 mM 0.1197 mL 0.5987 mL 1.1975 mL 2.9937 mL
80 mM 0.0898 mL 0.4491 mL 0.8981 mL 2.2453 mL
100 mM 0.0718 mL 0.3592 mL 0.7185 mL 1.7962 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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S-Methylisothiourea sulfate
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