lysosomes kit

The discovery of hit molecule is a cornerstone of drug development. Among the diverse tools available, DNA-encoded libraries have emerged a revolutionary platform for high-throughput screening. Compared with traditional HTS, DEL features shorter screening processes, lower costs, simpler assays, and larger library capacities.

DEL Construction utilizes split-and-pool synthesis, a combinatorial chemistry approach that involves iterative splitting, reaction, and pooling. This strategy enables rapid, exponential assembly of fragments in minimal steps without the need for individual compound synthesis andassoicicated isolation or purification steps, thus greatly reducing overall costs. The technology enables simultaneous affinity screeningof massive compound collections to target proteins in a single step. By coupling chemical structures with unique DNA barcodes, each compound is tagged with a distinct DNA sequence for convenient tracking and decoding.DELs readily enable the construction and efficient screening of libraries containing millions to billions of compounds. As a result, DEL screening combines the dual advantages of high efficiency and low cost, making DEL a transformative technology in modern drug discovery.

The DEL kit consists of 50 independent libraries with a total scale of 100 billion compounds. It is constructed through stepwise combinatorial chemistry strategies involving 2-, 3-, and 4-round synthesis. By employing diverse scaffolds and flexible linking strategies, it encompasses various ring systems, linear frameworks, and heterocyclic structures. Screening can be achieved solely through affinity, independent of target-specific activity detection methods. This library is suitable for DEL screening against a wide range of targets.

Cyclic peptide library have advantages such as high affinity, high selectivity, and suitability for targeting protein–protein interactions. Through DEL synthesis technology, the library size can achieve hundreds of millions. DEL cyclic peptide library have advantages like low cost andhigh screeing efficiency, making them valuable for discovering lead compounds against challenging drug targets.

This cyclic peptide library is constructed with unnatural amino acids as building block, synthesized through DNA-compatible chemical reactions. Each cyclic peptide consist of six amino acids and constrained conformations such as side-chain cross-linking, disulfide bonds, and macrocyclization. These cyclic peptides exhibit significantly improved stability and druggability compared with linear peptides, filling the gap between small molecules and macromolecular biologics. Each cyclic peptide is uniquely conjugated to a DNA barcode sequence for molecular identification and sequencing decoding.

MCE’s cyclic peptide library has8 independent sub-libraries, with a total molecular diversity of 1.2 billion. It is constructed via multi-round combinatorial assembly of building blocks and diverse cyclization strategies, facilitating the discovery of cyclic peptide leads for undruggable targets.

Autophagy is a lysosomal degradation pathway that is essential for cell survival, differentiation, development, and homeostasis. The process of autophagy in mammalian cells is as follows: a portion of cytoplasm, including organelles, is enclosed by a phagophore or isolation membrane to form an autophagosome. The outer membrane of the autophagosome subsequently fuses with the endosome and then the lysosome, and the internal material is degraded. Autophagy plays a wide variety of physiological and pathophysiological roles. Defective autophagy contributes to various pathologies, including infections, cancer, neurodegeneration, aging, and heart disease.

MCE provides a unique collection of 1,956 autophagy pathway-related compounds that is a useful tool for the research of autophagy-related regulation and diseases.

Cell death plays a crucial role in the development of the body and the maintenance of internal balance to prevent the development of diseases. According to the regulation of the involved processes, cell death can be defined as programmed and non-programmed death. Programmed cell death (PCD) can be divided into lytic cell death and nonlytic cell death, mainly including apoptosis, necrotic apoptosis and Pyroptosis. Non-Programmed cell death (Non-PCD) generally refers to necrosis. In stark contrast to Accidental Cell Death (ACD), Regulatory Cell Death (RCD) relies on specialized molecular mechanisms. Cell death includes internal apoptosis, external apoptosis, necrotic apoptosis, ferroptosis, pyroptosis, lysosome-dependent cell death, etc.

MCE designs a unique collection of 3,640 cell death compounds, covering multiple targets, such as Apoptosis, Ferroptosis, Pyroptosis, Necroptosis, etc. It is a useful tool for screening cell death drugs.

Protein ubiquitination is an enzymatic post-translational modification in which an ubiquitin protein is attached to a substrate protein. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. Ubiquitination affects cellular processes such as apoptosis, cell cycle, DNA damage repair, and membrane transportation, etc. by regulating the degradation of proteins (via the proteasome and lysosome), altering the cellular localization of proteins, affecting proteins activity, and promoting or preventing protein-protein interactions. Deregulation of ubiquitin pathway leads to many diseases such as neurodegeneration, cancer, infection and immunity, etc.

MCE offers a unique collection of 482 small molecule modulators with biological activity used for ubiquitination research. Compounds in this library target the key enzymes in ubiquitin pathway. MCE Ubiquitination Compound Library is a useful tool for the research of ubiquitination regulation and the corresponding diseases.

Lyso Green is a fluorescent probe with green fluorescence for specific labeling of lysosomes in living mammalian cells.

MCE Organoid Vitality Assay Kit is a fluorescence-based assay kit specifically designed for measuring cell viability in organoids.

MCE Mycoplasma Detection Kit (PCR) is a diagnostic kit that utilizes the PCR method to detect mycoplasma contamination in biological materials such as cultured cells.

MCE ATP Assay Kit provides a rapid method to measure intracellular ATP. The kit is suitable for ATP determination in cells, tissue, and other biological samples.

MCE Seamless DNA Assembly Ultra Kit is a next-generation recombinant cloning kit that allows the recombination of single or multiple DNA fragments in a single reaction.

The MCE three-color immunohistochemical kit marks the dual indicators. The kit contains rabbit secondary antibodies and can be used in combination with rabbit primary antibodies

The MCE four-color immunohistochemical kit can label three indicators. The kit contains rabbit secondary antibodies and can be used in combination with rabbit primary antibodies.

MCE Mycoplasma Detection Kit (qPCR) is a diagnostic kit that utilizes the real-time quantitative PCR (qPCR) method to detect mycoplasma contamination in biological materials such as cultured cells.

The MCE three-color immunohistochemical kit marks the dual indicators. The kit contains a universal secondary antibody for mice and rats and can be used in combination with rabbit or mouse primary antibodies.

The MCE four-color histochemical kit can label three indicators. The kit contains a universal secondary antibody for rabbits and mice and can be used in combination with primary antibodies for rabbits or mice.

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show green fluorescence.

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.

MCE Human iPSC/ESC Whole-Brain Organoid Induction Differentiation Kit is a non-region-specific brain organoid induction kit designed based on the neuroectoderm self-differentiation system. After 38 d of culture using this kit, the resulting whole-brain organoids stably express core neuronal markers such as TUJ1, SOX2, Nestin, and NeuN, as well as forebrain/cortical markers including FOXG1 and CTIP2.

MCE Mouse Intestinal Organoid Plus Kit is an upgraded serum-free culture system developed based on the Mouse Small Intestinal Organoid Culture Kit (HY-K6119), designed for the establishment, expansion, and long-term maintenance of mouse small intestinal organoids. The kit contains basal culture medium and multiple essential supplements, and an optional Wnt ligand supplement is provided to enable modulation of Wnt signaling activity in the culture system.

Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.The 5 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE ROS Assay Kit utilizes the fluorescent probe DCFH-DA to detect ROS.

MCE Lentivirus Packaging Kit is based on the second-generation lentiviral packaging system and is also compatible with both second- and third-generation packaging plasmids. Lentiviruses produced using this kit exhibit high infection efficiency and a broad host range, enabling stable and sustained expression of exogenous genes in host cells.

MCE Apoptosis and Necrosis Assay Kit provides a rapid and convenient method to detect cell apoptosis and necrosis.

MCE Gram Stain Kit can be used to differentiate between Gram-negative and Gram-positive bacteria.

MCE Cell Senescence β-Galactosidase Staining Kit can be used to evaluate the senescence of cells or tissues.

MCE Cell Apoptosis Analysis Kit (Hoechst staining) provides a rapid and convenient method to detect cell apoptosis.

MCE Antibody Labeling Kit-VF 488 can efficiently and covalently attach the VF 488 dye to antibodies.

MCE Human iPSC/ESC Cardiomyocyte Induction Differentiation Kit is based on the classical GiWi system. By precisely modulating the Wnt/β-catenin signaling pathway in a temporally controlled manner (sequential activation and inhibition), the kit enables highly efficient directed differentiation of human pluripotent stem cells into cardiomyocytes. It is suitable for cardiac disease modeling, drug cardiotoxicity assessment, and mechanistic studies.

MCE BCA Protein Assay Kit is a colorimetric assay based on the bicinchoninic acid (BCA) reaction principle for quantitative protein determination.

MCE Nitric Oxide Detection Kit employs a modified Griess method to detect the content of Nitric Oxide in various samples.

MCE offers a wide range of multiplex fluorescence kits for simultaneous detection of up to six targets to maximise customer satisfaction.

MCE Exosome Protein Detection Kit (CD81) can specifically detect the exosome marker protein CD81.

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.

MCE Mitochondria Isolation Kit for Tissue enables the fast and efficient isolation of intact mitochondria from tissue using differential centrifugation.

MCE VF 647A-Annexin V/PI Apoptosis Detection Kit can be utilized to detect the level of cell apoptosis.

MCE Bradford Protein Assay Kit is suitable for detecting the total protein content in samples such as serum, plasma, animal tissues, and cells.

MCE Biotin Apoptosis Detection Kit (TUNEL) can be used to detect apoptosis in cultured cells, as well as in frozen or paraffin sections.

MCE Fumarase Activity Assay Kit (WST-8) can be used to measure the amount of fumarase present in biological samples.

MCE Human Brain Organoid (Expansion) Kit contains Human Brain Organoid Expansion Basal Medium and Human Brain Organoid Expansion Culture Supplements. This kit enables the efficient in vitro generation of human forebrain organoids (hFBs). Within this culture system, human brain tissue can spontaneously form organoid structures that faithfully recapitulate key features of in vivo cellular heterogeneity and complex tissue organization.

MCE Mitochondria Isolation Kit for Cultured Cells enables the fast and efficient isolation of intact mitochondria from cells using differential centrifugation.

MCE Mycoplasma Detection Kit (Luminescence) utilizes chemiluminescence technology to detect the activity of specific enzymes in the sample, determining the presence of mycoplasma contamination.

MCE Annexin V-FITC/7-AAD Apoptosis Detection Kit can be utilized to detect the occurrence of early apoptosis.

MCE Exosome Isolation and Purification Kit (From Milk) provides a simple and effective method to isolate and purify intact exosomes from milk.

MCE Plant Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from various plant leaf samples. The kit enables rapid release of genomic DNA from plant tissues (such as rice, maize, tobacco, rapeseed, etc.) in a single step. The resulting lysate can be directly used for downstream PCR amplification without the need for conventional DNA extraction and purification, thereby significantly simplifying the experimental workflow.

MCE Caspase 3 Activity Assay Kit provides a simple and convenient way for detecting the activity of detection of Caspase 3 activity that based on the spectrophotometric.

MCE Caspase 1 Activity Assay Kit provides a simple and convenient way for detecting the activity of detection of Caspase 1 activity that based on the spectrophotometric.

MCE Human Lung Squamous Cell Carcinoma Organoid Kit can be used to efficiently construct human Lung Squamous Cell Carcinoma organoid.

MCE Neuroblastoma Organoid Kit contains Neuroblastoma organoid basal medium and culture supplement. This product can be used to efficiently construct Neuroblastoma organoid.

MCE Rhabdomyosarcoma Organoid Kit contains rhabdomyosarcoma organoid basal medium and culture supplement. This product can be used to efficiently construct rhabdomyosarcoma organoid.

MCE Hepatoblastoma Organoid Kit contains hepatoblastoma organoid basal medium and culture supplement. This product can be used to efficiently construct hepatoblastoma organoid.

MCE Lipid Peroxidation (MDA) Assay Kit is suitable for measuring MDA levels in a variety of samples including plasma, serum, urine, tissues or cell lysates.

MCE Mouse Neutrophil Negative Selection Kit can isolate neutrophils from single-cell suspensions of mouse bone marrow or other tissue samples.

MCE Human Brain (Differentiation) Organoid Kit includes a basic culture medium and differentiation supplements, designed for the induction and culture of human brain organoids (hFBs).

MCE Mycoplasma Detection Plus Kit (PCR) employs polymerase chain reaction (PCR) technology for rapid and sensitive detection of mycoplasma contamination in cultured cells and related biological samples. The kit contains a PCR premix and mycoplasma-specific primers designed against conserved regions of the 16S rRNA gene. Cell culture supernatant or cell lysates can be used directly as templates for specific amplification of mycoplasma DNA, enabling rapid identification of mycoplasma contamination.