Ammonium chloride, AR, 99.5%

Name: Ammonium chloride, AR, 99.5%
Brand: MedChemExpress
SKU: HY-Y1269
Rating: 5.0 (47 reviews)

Cat. No.: HY-Y1269 Assay: 99.67%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Ammonium chloride, as a heteropolar compound with pH value regulation, can cause intracellular alkalization and metabolic acidosis thus effecting enzymatic activity and influencing the process of biological system. Ammonium chloride is an autophagy inhibitor. Ammonium chloride is also a lysosome inhibitor.

For research use only. We do not sell to patients.

CAS No. : 12125-02-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Based on 47 publication(s) in Google Scholar

Other Forms of Ammonium chloride, AR, 99.5%:

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Ammonium chloride, United States Pharmacopeia (USP) Reference Standard Get quote

47 Publications Citing Use of MCE Ammonium chloride, AR, 99.5%

Bio/Physico-chemical Assay

: Ammonium chloride, AR, 99.5% purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 4:e04680. [Abstract]; Resistant cells were treated with 25 mM Ammonium chloride (NH₄Cl) alone or co‐treated with 80 µm FA, and the expression level of DDX6 was detected by immunoblotting, n = 3. The results showed that supplementation with the lysosomal inhibitor NH₄Cl could rescue DDX6 expression reduced by FA intervention.

: Ammonium chloride, AR, 99.5% purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 31;6(9):e70353. [Abstract]; HEK293T‐hACE2 cells were exposed to pseudotyped SARS‐COV‐2 (wild type and two variants: B.1.351 and B.1.671) or VSV (MOI = 10) in the presence of varying concentrations of tetrandrine or Ammonium chloride (NH₄Cl). Firefly luciferase activity, indicative of infection, was measured 24 h post‐infection and normalized to the mock‐treated control. *p < 0.05, **p < 0.01, ***p < 0.001 versus virus control. Mock, cell group. Con, virus‐infected group.

: Ammonium chloride, AR, 99.5% purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2025 Apr 10:217715. [Abstract]; Western blot was used to test the influence of Bufalin (100 nM) combined with Ammonium chloride (NH₄CL, 30 mM) or BafA1 (50 nM) for different time (0, 0.5, 1, 2 h) in KYSE30 and KYSE450. The results showed that Bufalin-induced EGFR degradation could be reversed by lysosomal protease NH₄CL.

: Ammonium chloride, AR, 99.5% purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2025 Jun 14:296:117871. [Abstract]; Immunoblots and quantification of EGFR in A549 cells treated with different concentrations of LY-dE#5, with or without MG132, CQ, and Ammonium chloride (NH₄Cl) (0.5-2 μM). The results showed that NH₄Cl significantly blocked LY-dE#5-mediated EGFR degradation.

: Ammonium chloride, AR, 99.5% purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2025 Jul 16;21(7):e1013344. [Abstract]; 293T cells were transfected with the pCAGGS-HA-ORF3a plasmid and subsequently treated with Ammonium chloride (NH₄Cl) (20 mM). Cells were harvested after 48 h, and the expression levels of endogenous YY1 were assessed by Western blot using either HA or YY1 antibodies.

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Ammonium chloride (NH₄Cl), a lysosomotropic agent that raises intralysosomal pH, reduces the yield of reovirus during infection of mouse L cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ammonium chloride (0.28 M in drinking water) promotes the survival of myocardial cells in vivo by decreasing contractile dysfunction, cardiac hypertrophy, inflammation, apoptosis and autophagy^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-9-week-old C57B/L6 mice^[1]
Dosage:	0.28 M in drinking water (5 mg/kg doxorubicin once a week for 2 weeks)
Administration:	0.28 M in drinking water (5 mg/kg doxorubicin once a week for 2 weeks)
Result:	Effectively improved doxorubicin (DOX)-induced cardiomyocyte apoptosis and cardiac dysfunction in mice.

Molecular Weight

53.49

Formula

NH₄Cl

CAS No.

12125-02-9

Appearance

Solid

Color

White to off-white

SMILES

[NH4].[Cl]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (1869.51 mM; Need ultrasonic)

DMSO : 50 mg/mL (934.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	18.6951 mL	93.4754 mL	186.9508 mL
5 mM	3.7390 mL	18.6951 mL	37.3902 mL
10 mM	1.8695 mL	9.3475 mL	18.6951 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (46.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (46.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (46.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (273 KB) SDS (536 KB)

COA (308 KB)

Handling Instructions (2659 KB)

References

[1]. Huang X, et al. NH4Cl treatment prevents doxorubicin-induced myocardial dysfunction in vivo. Life Sci. 2019;227:94-100. [Content Brief]

[2]. Canning WM, Fields BN. Ammonium chloride prevents lytic growth of reovirus and helps to establish persistent infection in mouse L cells. Science. 1983;219(4587):987-988. [Content Brief]

[3]. Yu C,et al. Lysosome dysfunction enhances oxidative stress-induced apoptosis through ubiquitinated protein accumulation in Hela cells. Anat Rec (Hoboken). 2013 Jan;296(1):31-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	18.6951 mL	93.4754 mL	186.9508 mL	467.3771 mL
	5 mM	3.7390 mL	18.6951 mL	37.3902 mL	93.4754 mL
	10 mM	1.8695 mL	9.3475 mL	18.6951 mL	46.7377 mL
	15 mM	1.2463 mL	6.2317 mL	12.4634 mL	31.1585 mL
	20 mM	0.9348 mL	4.6738 mL	9.3475 mL	23.3689 mL
	25 mM	0.7478 mL	3.7390 mL	7.4780 mL	18.6951 mL
	30 mM	0.6232 mL	3.1158 mL	6.2317 mL	15.5792 mL
	40 mM	0.4674 mL	2.3369 mL	4.6738 mL	11.6844 mL
	50 mM	0.3739 mL	1.8695 mL	3.7390 mL	9.3475 mL
	60 mM	0.3116 mL	1.5579 mL	3.1158 mL	7.7896 mL
	80 mM	0.2337 mL	1.1684 mL	2.3369 mL	5.8422 mL
	100 mM	0.1870 mL	0.9348 mL	1.8695 mL	4.6738 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.