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Ammonium chloride, for cell culture

(Synonyms: Salmiac, for cell culture)
62 Cited Publications
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Based on 62 publication(s) in Google Scholar

Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor.

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  • Purity: 99.0%
  • CAS No.: 12125-02-9
  • Formula: NH4Cl
  • Molecular Weight:53.49
  • Storage:

    4°C, sealed storage, away from moisture

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

  • Ammonium chloride, for cell culture Chemical Structure
    Ammonium chloride, for cell culture Chemical Structure
  • Experimental Validation Results for Ammonium chloride, for cell culture
  • Experimental Validation Results for Ammonium chloride, for cell culture
  • Experimental Validation Results for Ammonium chloride, for cell culture
  • Experimental Validation Results for Ammonium chloride, for cell culture
  • Experimental Validation Results for Ammonium chloride, for cell culture
CAS No.: 12125-02-9
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  • Ammonium chloride, for cell culture Chemical Structure
    Ammonium chloride, for cell culture Chemical Structure
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    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 4:e04680. [Abstract]

    Customer Validation
     Resistant cells were treated with 25 mM Ammonium chloride (NH4Cl) alone or co‐treated with 80 µm FA, and the expression level of DDX6 was detected by immunoblotting, n = 3. The results showed that supplementation with the lysosomal inhibitor NH4Cl could rescue DDX6 expression reduced by FA intervention.

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    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 31;6(9):e70353. [Abstract]

    Customer Validation
     HEK293T‐hACE2 cells were exposed to pseudotyped SARS‐COV‐2 (wild type and two variants: B.1.351 and B.1.671) or VSV (MOI = 10) in the presence of varying concentrations of tetrandrine or Ammonium chloride (NH4Cl). Firefly luciferase activity, indicative of infection, was measured 24 h post‐infection and normalized to the mock‐treated control. *p < 0.05, **p < 0.01, ***p < 0.001 versus virus control. Mock, cell group. Con, virus‐infected group.

  • 1/16

    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2025 Jul 28:623:217715. [Abstract]

    Customer Validation
     Western blot was used to test the influence of Bufalin (100 nM) combined with Ammonium chloride (NH4CL, 30 mM) or BafA1 (50 nM) for different time (0, 0.5, 1, 2 h) in KYSE30 and KYSE450. The results showed that Bufalin-induced EGFR degradation could be reversed by lysosomal protease NH4CL.

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    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2025 Oct 15:296:117871. [Abstract]

    Customer Validation
     Immunoblots and quantification of EGFR in A549 cells treated with different concentrations of LY-dE#5, with or without MG132, CQ, and Ammonium chloride (NH4Cl) (0.5-2 μM). The results showed that NH4Cl significantly blocked LY-dE#5-mediated EGFR degradation.

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    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2025 Jul 16;21(7):e1013344. [Abstract]

    Customer Validation
     293T cells were transfected with the pCAGGS-HA-ORF3a plasmid and subsequently treated with Ammonium chloride (NH4Cl) (20 mM). Cells were harvested after 48 h, and the expression levels of endogenous YY1 were assessed by Western blot using either HA or YY1 antibodies.

  • Ammonium chloride, for cell culture