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  4. Ammonium chloride, for cell culture

Ammonium chloride, for cell culture  (Synonyms: Salmiac, for cell culture)

Cat. No.: HY-Y1269C Purity: 99.0%
COA
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Handling Instructions Technical Support

Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor.

For research use only. We do not sell to patients.

CAS No. : 12125-02-9

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Ammonium chloride, for cell culture Related Antibodies

Top Publications Citing Use of Products

47 Publications Citing Use of MCE Ammonium chloride, for cell culture

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Bio/Physico-chemical Assay

    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 4:e04680.  [Abstract]

    Resistant cells were treated with 25 mM Ammonium chloride (NH4Cl) alone or co‐treated with 80 µm FA, and the expression level of DDX6 was detected by immunoblotting, n = 3. The results showed that supplementation with the lysosomal inhibitor NH4Cl could rescue DDX6 expression reduced by FA intervention.

    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 31;6(9):e70353.  [Abstract]

    HEK293T‐hACE2 cells were exposed to pseudotyped SARS‐COV‐2 (wild type and two variants: B.1.351 and B.1.671) or VSV (MOI = 10) in the presence of varying concentrations of tetrandrine or Ammonium chloride (NH4Cl). Firefly luciferase activity, indicative of infection, was measured 24 h post‐infection and normalized to the mock‐treated control. *p < 0.05, **p < 0.01, ***p < 0.001 versus virus control. Mock, cell group. Con, virus‐infected group.

    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2025 Apr 10:217715.  [Abstract]

    Western blot was used to test the influence of Bufalin (100 nM) combined with Ammonium chloride (NH4CL, 30 mM) or BafA1 (50 nM) for different time (0, 0.5, 1, 2 h) in KYSE30 and KYSE450. The results showed that Bufalin-induced EGFR degradation could be reversed by lysosomal protease NH4CL.

    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2025 Jun 14:296:117871.  [Abstract]

    Immunoblots and quantification of EGFR in A549 cells treated with different concentrations of LY-dE#5, with or without MG132, CQ, and Ammonium chloride (NH4Cl) (0.5-2 μM). The results showed that NH4Cl significantly blocked LY-dE#5-mediated EGFR degradation.

    Ammonium chloride, for cell culture purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2025 Jul 16;21(7):e1013344.  [Abstract]

    293T cells were transfected with the pCAGGS-HA-ORF3a plasmid and subsequently treated with Ammonium chloride (NH4Cl) (20 mM). Cells were harvested after 48 h, and the expression levels of endogenous YY1 were assessed by Western blot using either HA or YY1 antibodies.

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    Description

    Ammonium chloride, for cell culture (Salmiac, for cell culture) is a reagent that can be used in cell culture to provide a source of nitrogen. Ammonium chloride can be used as a heteropolar compound to regulate pH value, which can cause intracellular alkalination and metabolic acidosis, thus affecting the activity of enzymes and affecting the process of biological systems. Ammonium chloride acts as an autophagy inhibitor.Ammonium chloride is also a lysosome inhibitor.

    In Vitro

    Ammonium chloride (NH4Cl), a lysosomotropic agent that raises intralysosomal pH, reduces the yield of reovirus during infection of mouse L cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ammonium chloride, for cell culture Related Antibodies
    In Vivo

    Ammonium chloride (0.28 M in drinking water) promotes the survival of myocardial cells in vivo by decreasing contractile dysfunction, cardiac hypertrophy, inflammation, apoptosis and autophagy[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 8-9-week-old C57B/L6 mice[1].
    Dosage: 0.28 M
    Administration: Drinking water (5 mg/kg Doxorubicin once a week for 2 weeks)
    Result: Effectively improved Doxorubicin (DOX)-induced cardiomyocyte apoptosis and cardiac dysfunction in mice.
    Molecular Weight

    53.49

    Formula

    NH4Cl
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (1869.51 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 18.6951 mL 93.4754 mL 186.9508 mL
    5 mM 3.7390 mL 18.6951 mL 37.3902 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.0%

    SDS (537 KB)

    COA (244 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 18.6951 mL 93.4754 mL 186.9508 mL 467.3771 mL
    5 mM 3.7390 mL 18.6951 mL 37.3902 mL 93.4754 mL
    10 mM 1.8695 mL 9.3475 mL 18.6951 mL 46.7377 mL
    15 mM 1.2463 mL 6.2317 mL 12.4634 mL 31.1585 mL
    20 mM 0.9348 mL 4.6738 mL 9.3475 mL 23.3689 mL
    25 mM 0.7478 mL 3.7390 mL 7.4780 mL 18.6951 mL
    30 mM 0.6232 mL 3.1158 mL 6.2317 mL 15.5792 mL
    40 mM 0.4674 mL 2.3369 mL 4.6738 mL 11.6844 mL
    50 mM 0.3739 mL 1.8695 mL 3.7390 mL 9.3475 mL
    60 mM 0.3116 mL 1.5579 mL 3.1158 mL 7.7896 mL
    80 mM 0.2337 mL 1.1684 mL 2.3369 mL 5.8422 mL
    100 mM 0.1870 mL 0.9348 mL 1.8695 mL 4.6738 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Ammonium chloride, for cell culture Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ammonium chloride, for cell culture12125-02-9Salmiac, for cell cultureAutophagyBiochemical Assay ReagentsEnvironmental PollutantsEnvironmental ContaminantsheteropolarpHintralysosomalinflammationcardiacdysfunctionLysosomeNH4CLInhibitorinhibitorinhibit

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