2. PI3K/Akt/mTOR
    Autophagy
  3. mTOR
    Autophagy
  4. MHY1485

MHY1485 

Cat. No.: HY-B0795 Purity: 99.74%
COA Handling Instructions

MHY1485 is a potent cell-permeable mTOR activator that targets the ATP domain of mTOR. MHY1485 inhibits autophagy by suppression of fusion between autophagosomes and lysosomes.

For research use only. We do not sell to patients.

MHY1485 Chemical Structure

MHY1485 Chemical Structure

CAS No. : 326914-06-1

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 61 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 77 In-stock
Estimated Time of Arrival: December 31
50 mg USD 242 In-stock
Estimated Time of Arrival: December 31
100 mg USD 440 In-stock
Estimated Time of Arrival: December 31
200 mg USD 770 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
1 g   Get quote  

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Customer Review

Based on 88 publication(s) in Google Scholar

Top Publications Citing Use of Products

81 Publications Citing Use of MCE MHY1485

WB

    MHY1485 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Feb 13;10(2):140.   [Abstract]

    Sorted naive T cells are cultured with TGF-β, IL-2 with or without 40 μM Baicalin or 10 μM MHY1485 for 3 h, P-mTOR, p-S6K, P-4EBP1, and GAPDH expression are analyzed by western blot.

    MHY1485 purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Jul;119(7):6104-6112.  [Abstract]

    MHY-1485 significantly suppresses the increase in LC3II/LC3I ratios induced by Cucurbitacin I.

    MHY1485 purchased from MCE. Usage Cited in: Exp Cell Res. 2018 Jun 15;367(2):186-195.  [Abstract]

    MHY1485 prevents high glucose-caused reduced phospho-S6K1 (Thr 389) and cell apoptosis in RSC96 cells.

    MHY1485 purchased from MCE. Usage Cited in: Neurochem Res. 2018 Nov;43(11):2141-2154.  [Abstract]

    MHY1485 (M) increases phospho-mTOR (Ser 2448), phosphoS6K1 (Thr 389) and NGF expression in RSC96 cells.

    MHY1485 purchased from MCE. Usage Cited in: Exp Cell Res. 2017 Nov 15;360(2):328-336.  [Abstract]

    HT29 cells are co-treated with 5-FU and MHY1485 (MHY) for 48 h. Proteins are collected and immunoblotted with mTOR, p-p70S6K, and p70S6K, with β-actin as the loading control.

    MHY1485 purchased from MCE. Usage Cited in: Oncotarget. 2017 Feb 21;8(8):12775-12783.  [Abstract]

    OSI-027, AZD-8055 and AZD-2014 almost completely block MHY1485-induced mTOR activation (p-mTOR/S6K1/Akt Ser473) in skin keratinocytes.

    MHY1485 purchased from MCE. Usage Cited in: Biochem Pharmacol. 2016 Dec 15;122:42-61.  [Abstract]

    Inhibition of SREBPs processing by AHI is dependent on LKB-1/AMPK/mTOR pathway. (A) HepG2 cells are incubated with or without MHY1485 or Rapamycin for 1 h, the cells are switched to medium D in the presence of vehicle, or AHI. (B) HepG2 cells are incubated with or without Compound C for 1 h, the cells are switched to medium D in the presence of vehicle, or AHI.

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    mTOR mTORC1 mTORC2

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    Description

    MHY1485 is a potent cell-permeable mTOR activator that targets the ATP domain of mTOR. MHY1485 inhibits autophagy by suppression of fusion between autophagosomes and lysosomes[1].

    IC50 & Target

    mTORC1

     

    mTORC2

     

    Autophagy

     

    In Vitro

    MHY1485 (10 μM; 4 hours) shows that GCDC-induced autophagic activity is inhibited by upregulating p-mTOR expression and downregulating LC3 and p62 expression in HCC cells[1].
    MHY1485 (5 μM; 6 hours) increases the LC3II/LC3I ratio in a dose and time-dependently manner due to presumably inhibited LC3II degradation in rat liver Ac2F cells[2].
    MHY1485 (0.5-2 μM; 6 hours) increases the phosphorylation of mTOR at ser2448 and upregulates the level of phosphorylation of 4E-BP1 in a dose-dependently manner in Ac2F cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[1]

    Cell Line: HCC cells
    Concentration: 10 μM
    Incubation Time: 4 hours
    Result: Upregulated p-mTOR and downregulated LC3 and p62 expression.
    In Vivo

    MHY1485 (intraperitoneal injection; 10 mg/kg, 2 days) blocks the autophagy signaling induced by follicle-stimulating hormone (FSH). It increases p-mTOR and p-S6K1 expression levels, whereas LC3 expression shows no marked change compared to that in the control group[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 4-week-old female ICR mice[3]
    Dosage: 10 mg/kg, 2 days
    Administration: Intraperitoneal injection
    Result: Suppressed the autophagy level following FSH treatment. 
    Molecular Weight

    387.39

    Appearance

    Solid

    Formula

    C17H21N7O4
    CAS No.
    SMILES

    O=[N+](C1=CC=C(NC2=NC(N3CCOCC3)=NC(N4CCOCC4)=N2)C=C1)[O-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 7.69 mg/mL (19.85 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (ultrasonic) (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5814 mL 12.9069 mL 25.8138 mL
    5 mM 0.5163 mL 2.5814 mL 5.1628 mL
    10 mM 0.2581 mL 1.2907 mL 2.5814 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  50% PEG300    50% saline

      Solubility: 5 mg/mL (12.91 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  20% HP-β-CD in saline

      Solubility: 1 mg/mL (2.58 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 0.77 mg/mL (1.99 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 0.77 mg/mL (1.99 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.86%

    COA (252 KB) LCMS (115 KB)

    Handling Instructions (2659 KB)
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    MHY1485326914-06-1MHY 1485MHY-1485mTORAutophagyMammalian target of Rapamycinautophagycell-permeable MC3T3-E1cell FSHInhibitorinhibitorinhibit

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