1. PI3K/Akt/mTOR
    Autophagy
  2. mTOR
    Autophagy
  3. MHY1485

MHY1485 

Cat. No.: HY-B0795 Purity: 99.05%
Handling Instructions

MHY1485 is a potent cell-permeable mTOR activator that targets the ATP domain of mTOR. MHY1485 inhibits autophagy by suppression of fusion between autophagosomes and lysosomes

For research use only. We do not sell to patients.

MHY1485 Chemical Structure

MHY1485 Chemical Structure

CAS No. : 326914-06-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 70 In-stock
Estimated Time of Arrival: December 31
50 mg USD 220 In-stock
Estimated Time of Arrival: December 31
100 mg USD 400 In-stock
Estimated Time of Arrival: December 31
200 mg USD 700 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
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Customer Review

Based on 23 publication(s) in Google Scholar

Top Publications Citing Use of Products

    MHY1485 purchased from MCE. Usage Cited in: Biochem Pharmacol. 2016 Dec 15;122:42-61.

    Inhibition of SREBPs processing by AHI is dependent on LKB-1/AMPK/mTOR pathway. (A) HepG2 cells are incubated with or without MHY1485 or Rapamycin for 1 h, the cells are switched to medium D in the presence of vehicle, or AHI. (B) HepG2 cells are incubated with or without Compound C for 1 h, the cells are switched to medium D in the presence of vehicle, or AHI.

    MHY1485 purchased from MCE. Usage Cited in: Exp Cell Res. 2017 Nov 15;360(2):328-336.

    HT29 cells are co-treated with 5-FU and MHY1485 (MHY) for 48 h. Proteins are collected and immunoblotted with mTOR, p-p70S6K, and p70S6K, with β-actin as the loading control.

    MHY1485 purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Jul;119(7):6104-6112.

    MHY-1485 significantly suppresses the increase in LC3II/LC3I ratios induced by Cucurbitacin I.

    MHY1485 purchased from MCE. Usage Cited in: Exp Cell Res. 2018 Jun 15;367(2):186-195.

    MHY1485 prevents high glucose-caused reduced phospho-S6K1 (Thr 389) and cell apoptosis in RSC96 cells.

    MHY1485 purchased from MCE. Usage Cited in: Neurochem Res. 2018 Nov;43(11):2141-2154.

    MHY1485 (M) increases phospho-mTOR (Ser 2448), phosphoS6K1 (Thr 389) and NGF expression in RSC96 cells.

    MHY1485 purchased from MCE. Usage Cited in: Oncotarget. 2017 Feb 21;8(8):12775-12783.

    OSI-027, AZD-8055 and AZD-2014 almost completely block MHY1485-induced mTOR activation (p-mTOR/S6K1/Akt Ser473) in skin keratinocytes.

    MHY1485 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Feb 13;10(2):140. 

    Sorted naive T cells are cultured with TGF-β, IL-2 with or without 40 μM Baicalin or 10 μM MHY1485 for 3 h, P-mTOR, p-S6K, P-4EBP1, and GAPDH expression are analyzed by western blot.

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    Description

    MHY1485 is a potent cell-permeable mTOR activator that targets the ATP domain of mTOR. MHY1485 inhibits autophagy by suppression of fusion between autophagosomes and lysosomes[1]

    IC50 & Target[4]

    mTORC1

     

    mTORC2

     

    Autophagy

     

    In Vitro

    MHY1485 (10 μM; 4 hours) shows that GCDC-induced autophagic activity is s inhibited by upregulating p-mTOR expression and downregulating LC3 and p62 expression in HCC cells[1].
    MHY1485 (5 μM; 6 hours) increases the LC3II/LC3I ratio in a dose and time-dependently manner due to presumably inhibited LC3II degradation in rat liver Ac2F cells[2].
    MHY1485 (0.5-2 μM; 6 hours) increases the phosphorylation of mTOR at ser2448 and upregulates the level of phosphorylation of 4E-BP1 in a dose-dependently manner in Ac2F cells[2].

    Western Blot Analysis[1]

    Cell Line: HCC cells
    Concentration: 10 μM
    Incubation Time: 4 hours
    Result: Upregulated p-mTOR and downregulated LC3 and p62 expression.
    Molecular Weight

    387.39

    Formula

    C₁₇H₂₁N₇O₄

    CAS No.

    326914-06-1

    SMILES

    O=[N+](C1=CC=C(NC2=NC(N3CCOCC3)=NC(N4CCOCC4)=N2)C=C1)[O-]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 9.5 mg/mL (24.52 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5814 mL 12.9069 mL 25.8138 mL
    5 mM 0.5163 mL 2.5814 mL 5.1628 mL
    10 mM 0.2581 mL 1.2907 mL 2.5814 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
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    Product name:
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    Cat. No.:
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