2. GPCR/G Protein Apoptosis
  3. LPL Receptor Apoptosis
  4. W146 TFA

W146 TFA is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50 value of 398 nM.

For research use only. We do not sell to patients.

W146 TFA Chemical Structure

W146 TFA Chemical Structure

CAS No. : 909725-62-8

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Based on 1 publication(s) in Google Scholar

Other Forms of W146 TFA:

W146 TFA Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE W146 TFA

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LPAR1 LPAR2 LPAR3 LPAR5 S1PR1 S1PR2 S1PR3 S1PR4 S1PR5

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

W146 TFA is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50 value of 398 nM.

IC50 & Target

EC50: 398 nM (S1PR1)[1].
In Vitro

W146 is a S1PR1 antagonist with a Ki of ~70-80 nM[1].
W146 pretreatment significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

W146 TFA Related Antibodies

Apoptosis Analysis[2]

Cell Line: Endothelial progenitor cells (EPCs).
Concentration: 10 μM.
Incubation Time: 30 min before the addition of S1P.
Result: Increases activated cleaved caspase-3 levels.
In Vivo

W146 (5 mg/kg, ip, prior to AMD3100 administration) pre-treatment shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone[3]
The W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization. Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice (4-6-week-old)[3]
.
Dosage: 5 mg/kg.
Administration: IP, 1 hour prior to AMD3100 (ADM) administration.
Result: Significantly increased in KSL-HSPC mobilization compared to that in mice pretreated with dextran followed by AMD3100 administration.
Molecular Weight

456.39

Formula

C18H28F3N2O6P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=CC(CCCCCC)=C1)[C@H](N)CCP(O)(O)=O.O=C(O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 22.73 mg/mL (49.80 mM; ultrasonic and warming and adjust pH to 3 with 1M HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1911 mL 10.9555 mL 21.9111 mL
5 mM 0.4382 mL 2.1911 mL 4.3822 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.13%

COA (246 KB) HNMR (349 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1911 mL 10.9555 mL 21.9111 mL 54.7777 mL
5 mM 0.4382 mL 2.1911 mL 4.3822 mL 10.9555 mL
10 mM 0.2191 mL 1.0956 mL 2.1911 mL 5.4778 mL
15 mM 0.1461 mL 0.7304 mL 1.4607 mL 3.6518 mL
20 mM 0.1096 mL 0.5478 mL 1.0956 mL 2.7389 mL
25 mM 0.0876 mL 0.4382 mL 0.8764 mL 2.1911 mL
30 mM 0.0730 mL 0.3652 mL 0.7304 mL 1.8259 mL
40 mM 0.0548 mL 0.2739 mL 0.5478 mL 1.3694 mL
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W146 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

W146909725-62-8W 146W-146LPL ReceptorApoptosisLysophospholipid ReceptorS1PRKSLHSPClymphocyteAktLPLInhibitorinhibitorinhibit

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