α-Hexylcinnamaldehyde

Name: α-Hexylcinnamaldehyde
Brand: MedChemExpress
SKU: HY-W014118
Rating: 4.5 (1 reviews)

Cat. No.: HY-W014118 Purity: 92.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

α-Hexylcinnamaldehyde is an O-acetyltransferase (OAT) inhibitor. α-Hexylcinnamaldehyde inhibits OAT-mediated bioactivation of nitroarene mutagens, exerts antimutagenic activity through demutagenic and bioantimutagenic mechanisms, and interferes with ATP-binding cassette (ABC) transporter function to reduce substrate efflux. α-Hexylcinnamaldehyde alters membrane permeability, fluidizes phospholipid membranes, exerts antioxidant effects, and enhances the antiproliferative effect of Doxorubicin on human cancer cells. α-Hexylcinnamaldehyde can be used in the research of colorectal adenocarcinoma, T-cell leukemia, and multidrug-resistant cancers.

For research use only. We do not sell to patients.

α-Hexylcinnamaldehyde Chemical Structure

CAS No. : 101-86-0

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of α-Hexylcinnamaldehyde:

α-Hexylcinnamaldehyde-d₅ Get quote
α-Hexylcinnamaldehyde (Standard) Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

α-Hexylcinnamaldehyde (Compound 1) (9-900 μM; 30 min treatment step, 72 h plate incubation) potently inhibits 2-nitrofluorene-induced mutagenicity in *Salmonella typhimurium* TA98; in the pre-treatment, co-treatment and post-treatment protocols, the maximum inhibition rates at the concentration of 900 μM reach 64.6%, 72.6% and 63.1%, respectively^[1].
α-Hexylcinnamaldehyde (9-900 μM; 30 min treatment step, 72 h plate incubation) potently inhibits 1-nitropyrene-induced mutagenicity in *Salmonella typhimurium* TA98; under pre-treatment, co-treatment and post-treatment protocols, the maximum inhibition rates at the concentration of 900 μM reach 57.9%, 82.1% and 65.8%, respectively^[1].
α-Hexylcinnamaldehyde (9-900 μM; 30 min treatment step, 72 h plate incubation) strongly inhibits 1,8-dinitropyrene-induced mutagenicity in *Salmonella typhimurium* TA98 under the co-treatment protocol, but only exhibits moderate inhibition under the pre-treatment and post-treatment protocols; at the concentration of 900 μM, the inhibition rates reach maximum values of 28.0%, >50% and 37.4% for the three protocols, respectively^[1].
α-Hexylcinnamaldehyde (9-900 μM; 30 min treatment step, 72 h plate incubation) potently inhibits 2-aminoanthracene-induced mutagenicity in *Salmonella typhimurium* TA98 under the combined treatment protocol, with a maximum inhibition rate of up to 61.9% at 900 μM, whereas only weak inhibitory effects are observed under the pre-treatment and post-treatment protocols^[1].
α-Hexylcinnamaldehyde (9-900 μM; 30 min treatment step, 72 h plate incubation) slightly inhibits the mutagenicity of 4-nitroquinoline-N-oxide-induced *Salmonella typhimurium* TA98, with a maximum inhibition rate of 20.6% at the concentration of 900 μM in the pre-treatment protocol^[1].
α-Hexylcinnamaldehyde (9-900 μM; 30 min treatment step, 72 h plate incubation) moderately inhibits methyl methanesulfonate-induced mutagenicity in *Escherichia coli* WP2uvrA, reaching a maximum inhibition rate of approximately 33% at 90 μM^[1].
α-Hexylcinnamaldehyde (9-900 μM; 30 min treatment step, 72 h plate incubation) strongly inhibits 1-nitropyrene-induced mutagenicity in the nitroreductase-deficient Salmonella typhimurium TA98NR strain under the co-treatment protocol, but only exerts weak to moderate inhibitory effects under the pre-treatment and post-treatment protocols; the maximum inhibition rate reaches approximately 86% at 90 μM under co-treatment, whereas the inhibition rates are 46.9% and 42.1% at 900 μM under pre-treatment and post-treatment, respectively^[1].
α-Hexylcinnamaldehyde (HCA) exhibits low direct cytotoxicity against Caco-2, CCRF/CEM, and CEM/ADR5000 human cancer cells, with IC₅₀ values of 325.2 μM, 205.7 μM, and 274.7 μM, respectively^[2].
α-Hexylcinnamaldehyde (50-181 μM) acts as a synergistic chemosensitizer for Doxorubicin (HY-15142A) in human cancer cell lines Caco-2, CCRF/CEM and CEM/ADR5000. At its IC₂₀ concentration, it reduces the IC₅₀ value of Doxorubicin in CCRF/CEM cells by up to 50.9-fold^[2].
α-Hexylcinnamaldehyde (20-320 μM) inhibits ABC transporter-mediated efflux of rhodamine 123 in Caco-2 and CEM/ADR5000 human cancer cells, but has no effect on efflux in CCRF/CEM cells^[2].
α-Hexylcinnamaldehyde (5-500 μg/mL; 180 min at 37 °C) exhibits no pro-oxidant activity in either hydrophilic or lipophilic environments over the concentration range of 5 to 500 μg/mL^[3].
α-Hexylcinnamaldehyde (5-500 μg/mL; 180 min at 37 °C) exhibits dose-dependent antioxidant activity against AAPH (HY-Y0525)-induced oxidation in a hydrophilic environment, achieving nearly complete inhibition at a concentration of 500 μg/mL, but shows no antioxidant activity in a lipophilic environment^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

216.32

Formula

C₁₅H₂₀O

CAS No.

101-86-0

Appearance

Liquid (Density: 0.954±0.06 g/cm³)

Color

Colorless to light yellow

SMILES

O=C/C(CCCCCC)=C\C1=CC=CC=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (462.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6228 mL	23.1139 mL	46.2278 mL
5 mM	0.9246 mL	4.6228 mL	9.2456 mL
10 mM	0.4623 mL	2.3114 mL	4.6228 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥92.0%

Select Batch:

Data Sheet (276 KB) SDS (597 KB)

COA (245 KB) HNMR (124 KB) RP-HPLC (113 KB)

Handling Instructions (2659 KB)

References

[1]. Di Giacomo S, et al. α-Hexylcinnamaldehyde inhibits the genotoxicity of environmental pollutants in the bacterial reverse mutation assay. J Nat Prod. 2014;77(12):2664-2670.

[2]. DI Giacomo S, et al. α-Hexylcinnamaldehyde Synergistically Increases Doxorubicin Cytotoxicity Towards Human Cancer Cell Lines. Anticancer Res. 2016;36(7):3347-3351.

[3]. Sarpietro MG, et al. Interaction of α-Hexylcinnamaldehyde with a Biomembrane Model: A Possible MDR Reversal Mechanism. J Nat Prod. 2015;78(5):1154-1159. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.6228 mL	23.1139 mL	46.2278 mL	115.5695 mL
	5 mM	0.9246 mL	4.6228 mL	9.2456 mL	23.1139 mL
	10 mM	0.4623 mL	2.3114 mL	4.6228 mL	11.5570 mL
	15 mM	0.3082 mL	1.5409 mL	3.0819 mL	7.7046 mL
	20 mM	0.2311 mL	1.1557 mL	2.3114 mL	5.7785 mL
	25 mM	0.1849 mL	0.9246 mL	1.8491 mL	4.6228 mL
	30 mM	0.1541 mL	0.7705 mL	1.5409 mL	3.8523 mL
	40 mM	0.1156 mL	0.5778 mL	1.1557 mL	2.8892 mL
	50 mM	0.0925 mL	0.4623 mL	0.9246 mL	2.3114 mL
	60 mM	0.0770 mL	0.3852 mL	0.7705 mL	1.9262 mL
	80 mM	0.0578 mL	0.2889 mL	0.5778 mL	1.4446 mL
	100 mM	0.0462 mL	0.2311 mL	0.4623 mL	1.1557 mL