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CASIN 

Cat. No.: HY-12874 Purity: 99.82%
COA Handling Instructions

CASIN is a selective GTPase Cdc42 inhibitor with an IC50 of 2 uM. CASIN can be used for the research of cancer.

For research use only. We do not sell to patients.

CASIN Chemical Structure

CASIN Chemical Structure

CAS No. : 425399-05-9

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Solution
10 mM * 1 mL in DMSO USD 101 In-stock
Estimated Time of Arrival: December 31
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ready for reconstitution
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10 mg USD 158 In-stock
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25 mg USD 317 In-stock
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50 mg USD 581 In-stock
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100 mg USD 950 In-stock
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Description

CASIN is a selective GTPase Cdc42 inhibitor with an IC50 of 2 uM. CASIN can be used for the research of cancer[1][2].

IC50 & Target

IC50: 2 μM (GTPase Cdc42)[1]

In Vitro

CASIN (0-10 μM; 3 d) inhibits the growth of AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells[1].
CASIN (0-10 μM; 16 h) inhibits melphalan induced FANCD2 mono-ubiquitination in LR5 cells but shows no effect to S cells[1].
CASIN (5 μM; 2 d) increases cell apoptosis of bortezomib-resistant MM patient cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells
Concentration: 0-10 μM
Incubation Time: 3 days
Result: Inhibited MM cell lines including AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells with GI50s vof 3.23, 4.31, 4.87, 2.56, 4.64, 4.67 and 3.97 μM, respectively.

Western Blot Analysis[3]

Cell Line: MM (S) and melphalan-resistant (LR5) MM cells
Concentration: 5 μM
Incubation Time: 16 hours
Result: Abolished melphalan induced FANCD2 mono-ubiquitination and DNA damage in LR5, sensitized LR5 to melphalan-induced cell death by blocking FANCD2-mediated DNA damage repair.

Apoptosis Analysis[3]

Cell Line: Bortezomib-resistant MM patients cells
Concentration: 5 μM
Incubation Time: 2 days
Result: 2-fold increased cell apoptosis in the cell derived from bortezomib-resistant MM patients.
In Vivo

CASIN (20 mg/kg; i.p. 2 times a day) prolongs lifetime of mice with busulfan injection[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NSG mice with busulfan injection[3]
Dosage: 20 mg/kg
Administration: Intraperitoneal injection; 20 mg/kg 2 times a day
Result: Significantly prolonged the lifespan of xenografts mice better than bortezomib, and showed a leading position when combined with bortezomib.
Molecular Weight

306.40

Formula

C20H22N2O

CAS No.
SMILES

OCCNC1C(NC2=C3C=C(C4=CC=CC=C4)C=C2)=C3CCC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 31 mg/mL (101.17 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2637 mL 16.3185 mL 32.6371 mL
5 mM 0.6527 mL 3.2637 mL 6.5274 mL
10 mM 0.3264 mL 1.6319 mL 3.2637 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.82%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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