2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. FIPI

FIPI  (Synonyms: 5-Fluoro-2-indolyl deschlorohalopemide)

製品番号: HY-12807 純度: 98.00%
COA 取扱説明書 Technical Support

FIPI is a phospholipase D (PLD) inhibitor with an IC50 for PLD1 and PLD2 of about 25 nM. FIPI regulates cytoskeletal recombination, cell diffusion and chemotaxis. FIPI can be used in cancer research. In addition, FIPI can enhance the secretion and aggregation of platelet dense particles, inhibit thrombosis, reduce ischemic stroke infarct volume and improve nerve function.

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CAS 番号 : 939055-18-2

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
5 mg $85 在庫あり
10 mg $110 在庫あり
25 mg $230 お問い合わせ
50 mg $385 在庫あり
100 mg $600 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 5 publication(s) in Google Scholar

Other Forms of FIPI:

FIPI 関連抗体

Top Publications Citing Use of Products

    FIPI purchased from MedChemExpress. Usage Cited in: Anal Chem. 2018 Jun 5;90(11):6742-6748.  [Abstract]

    Typical CSLM images of MDA-MB-231 cells incubated with 50 μg/mL RhB-PL-Ti3C2 for 4 h at 37°C in the absence (I) and presence of 1.0 mg/mL FIPI (II), and CSLM images of MCF-7 cells (III) incubated with 50 μg/mL RhB-PL-Ti3C2 for 4 h at 37°C.

    FIPI purchased from MedChemExpress. Usage Cited in: J Cell Sci. 2018 Mar 16;131(6). pii: jcs207217.  [Abstract]

    ER:Ras IMR90 cells are treated with solvent or FIPI (250 nM, 2h).Western blotting are performed.

    Phospholipase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Phospholipase A PLD Phospholipase C

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    FIPI is a phospholipase D (PLD) inhibitor with an IC50 for PLD1 and PLD2 of about 25 nM. FIPI regulates cytoskeletal recombination, cell diffusion and chemotaxis. FIPI can be used in cancer research. In addition, FIPI can enhance the secretion and aggregation of platelet dense particles, inhibit thrombosis, reduce ischemic stroke infarct volume and improve nerve function[1][2][3][4].

    IC50 & Target[1]

    PLD1

     

    PLD2

     

    Cellular Effect
    Cell Line Type Value Description References
    Calu-1 IC50
    1 nM
    Compound: 14
    Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    		[PMID: 19136975]
    HEK293 IC50
    300 nM
    Compound: 14
    Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
    		[PMID: 19136975]
    体外実験

    FIPI (750 nM; 30 min) effectively blocks the production of phosphatidic acid by PLD stimulated by PMA (HY-18739) in HEK293 cells[1].
    FIPI (750 nM; 1-4 h) promotes the diffusion of CHO cells and inhibits the chemotaxis of neutrophils[1].
    FIPI (1-100 nM; 1-45 min) inhibits cell migration and calcium release in EGF-induced MDA-MB-468-NEO (EGFR positive) and MDA-MB-468-HER2 (EGFR and HER2 positive)[2].
    FIPI (10 μM; 2.5 min) enhances the secretion and aggregation of dense particles in platelets, and increases the phosphorylation of protein kinase C substrate Pleckstrin[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    FIPI 関連抗体
    体内実験

    FIPI (3 mg/kg; intraperitoneal injection; 2 times) shows protective effect in carotid thrombosis model and ischemic stroke model[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ferric chloride (HY-Y0266) or transient middle cerebral artery occlusion treated mice[4]
    Dosage: 3 mg/kg
    Administration: Intraperitoneal injection (i.p.); 2 times, 12 h apart
    Result: Delayed the thrombus formation and only 3 of 9 vessels occluded during the 30-minute observation period in arterial thrombosis models.
    Reduced the infarct volumes, improved motor and overall nerve function and did not impair normal hemostasis in ischemic stroke models.
    分子量

    421.47

    分子式

    C23H24FN5O2
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to khaki

    SMILES

    O=C(NCCN1CCC(CC1)N2C3=CC=CC=C3NC2=O)C4=CC5=C(C=CC(F)=C5)N4
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 3.33 mg/mL (7.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3726 mL 11.8632 mL 23.7265 mL
    5 mM 0.4745 mL 2.3726 mL 4.7453 mL
    10 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3726 mL 11.8632 mL 23.7265 mL 59.3162 mL
    5 mM 0.4745 mL 2.3726 mL 4.7453 mL 11.8632 mL
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    FIPI Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    FIPI939055-18-25-Fluoro-2-indolyl deschlorohalopemidePhospholipaseCarotid thrombosis modelIschemic stroke modelHEK293 cellsCHO cellsMDA-MB-468 cellsInhibitorinhibitorinhibit

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    製品名:
    FIPI
    製品番号:
    HY-12807
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