Haloperidol hydrochloride

Name: Haloperidol hydrochloride
Brand: MedChemExpress
SKU: HY-14538A
Rating: 5.0 (21 reviews)

Cat. No.: HY-14538A Purity: 99.56%: Data Sheet
COA

SDS
Handling Instructions
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Haloperidol hydrochloride is a potent dopamine D2 receptor antagonist, widely used as an antipsychotic agent. Haloperidol hydrochloride can be used in the study of schizophrenia, tics in Tourette syndrome, mania in bipolar disorder, delirium, agitation, acute psychosis, and hallucinations from alcohol withdrawal.

For research use only. We do not sell to patients.

CAS No. : 1511-16-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of Haloperidol hydrochloride:

21 Publications Citing Use of MCE Haloperidol hydrochloride

Cell Proliferation/Viability Assay

Cell Imaging/Staining

: Haloperidol hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep;12(33):e02276. [Abstract]; Western blotting of DRD1, ALP, OCN, BAX, Bcl2, cleaved‐caspase3, and β‐actin in osteogenesis‐induced BMSCs treated with Haloperidol (1 µM), DA and then stimulated with MP.

: Haloperidol hydrochloride purchased from MedChemExpress. Usage Cited in: ACS Omega. 2025 Oct 17;10(42):50208-50217. [Abstract]; Haloperidol (Halo; 20 ng/mL; 24 h). ISR induction by various drugs. N2a cells were treated with the indicated drugs for 24 h. The cell extracts were analyzed by Western blot analysis with anti-ATF4, anti-CHOP, and anti-β actin antibodies.

: Haloperidol hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Exp Pharmacol Physiol. 2021 Mar;48(3):370-380. [Abstract]; Haloperidol (10 μM; 48 h). Cell viability was measured by CCK-8 assay. The cells incubated with control medium were considered 100% viable.

: Haloperidol hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Exp Pharmacol Physiol. 2021 Mar;48(3):370-380. [Abstract]; Haloperidol (10 μM; 48 h). ROS production in HSCs measured by staining with DCFH-DA and imaged by fluorescent microscopy.

: Haloperidol hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Exp Pharmacol Physiol. 2021 Mar;48(3):370-380. [Abstract]; Haloperidol (10 μM; 48 h). Immunoblotting and optical density analysis of DRD2 and NOX-5, and the data were normalized to GAPDH.

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Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

D₂ Receptor

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Haloperidol can be used to create tardive dyskinesia models. In rats, the oral half-life of Haloperidol is 5.9 hours, with a Tmax of 0.9 hours, a Cmax of 6.8 ng/mL, and an oral bioavailability of 23.0%. When administered intravenously, the AUC_0-∞ of Haloperidol is 188.4 ng·h/mL, and the t_1/2 is 2.9 hours^[3].

Induction oftardive dyskinesia^[4]

Background

Haloperidolreduces gene expression of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GHP), thus reducing the complex cellular antioxidant defenses and increasing lipid peroxidation and nitrite/nitrate levels in the prefrontal cortex, hippocampus, and striatum^[4].

Specific Modeling Methods

Rat: 200-350 g • male Wistar
Administration: 1 mg/KG • i.p. • daily for 31 days

Modeling Indicators

Behavior: Showed a significant reduction of locomotor activity in the open field test and also developed a cataleptic state, vacuous chewing movements (VCM), and tongue protrusion.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

412.33

Formula

C₂₁H₂₄Cl₂FNO₂

CAS No.

1511-16-6

Appearance

Solid

Color

White to off-white

SMILES

ClC(C=C1)=CC=C1C2(O)CCN(CCCC(C3=CC=C(F)C=C3)=O)CC2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (121.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4252 mL	12.1262 mL	24.2524 mL
5 mM	0.4850 mL	2.4252 mL	4.8505 mL
10 mM	0.2425 mL	1.2126 mL	2.4252 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 10 mg/mL (24.25 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (12.13 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (12.13 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.56%

Select Batch:

Data Sheet (278 KB) SDS (645 KB)

COA (246 KB) HNMR (141 KB) RP-HPLC (162 KB) LCMS (388 KB)

Handling Instructions (2659 KB)

References

[1]. Joy CB, et al. Haloperidol versus placebo for schizophrenia. Cochrane Database Syst Rev. 2006 Oct 18;(4):CD003082. [Content Brief]

[2]. Giannini AJ, et al. Acute ketamine intoxication treated by haloperidol: a preliminary study. Am J Ther. 2000 Nov;7(6):389-91. [Content Brief]

Animal Administration
^[2]

Male albino mice of Swiss-Webster strain (33-36 g) are used, and all substances are given by i.p. injection in a volume of 0.5 mL. CPZ, haloperidoi and mescaline are all in time form of timeir imydrochlorides and the dose solutions are prepared at concentrations of 1.0, 0.66 and 3.3 mg/mL of 0.9% saline, respectively. The doses are: CPZ, 15 mg/kg; haloperidol, 10 mg/kg; mescaline, 50 mg/kg. Mice are pretreated with either CPZ or haloperidol 30 minutes before administration of mescaline. In some instances CPZ is injected 45 minutes after mescaline. Time animals are hmoused individually in a plexiglas cage and the gross behavior and locomotor activity. At selected intervals after mescaline, groups of mice are sacrificed by decapitation. Plasma is separated and stored at -20°C. The brain, liver, kidney, lung, spleen and heart are frozen on dry ice and stored at -20°C for 18 to 20 hours before they are used for assays.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Joy CB, et al. Haloperidol versus placebo for schizophrenia. Cochrane Database Syst Rev. 2006 Oct 18;(4):CD003082. [Content Brief]

[2]. Giannini AJ, et al. Acute ketamine intoxication treated by haloperidol: a preliminary study. Am J Ther. 2000 Nov;7(6):389-91. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4252 mL	12.1262 mL	24.2524 mL	60.6310 mL
	5 mM	0.4850 mL	2.4252 mL	4.8505 mL	12.1262 mL
	10 mM	0.2425 mL	1.2126 mL	2.4252 mL	6.0631 mL
	15 mM	0.1617 mL	0.8084 mL	1.6168 mL	4.0421 mL
	20 mM	0.1213 mL	0.6063 mL	1.2126 mL	3.0316 mL
	25 mM	0.0970 mL	0.4850 mL	0.9701 mL	2.4252 mL
	30 mM	0.0808 mL	0.4042 mL	0.8084 mL	2.0210 mL
	40 mM	0.0606 mL	0.3032 mL	0.6063 mL	1.5158 mL
	50 mM	0.0485 mL	0.2425 mL	0.4850 mL	1.2126 mL
	60 mM	0.0404 mL	0.2021 mL	0.4042 mL	1.0105 mL
	80 mM	0.0303 mL	0.1516 mL	0.3032 mL	0.7579 mL
	100 mM	0.0243 mL	0.1213 mL	0.2425 mL	0.6063 mL