Kinetin
Based on 7 publication(s) in Google Scholar
Kinetin (N6-furfuryladenine) belongs to the family of N6-substituted adenine derivatives known as cytokinins, which are plant hormones involved in cell division, differentiation and other physiological processes. Kinetin has anti-aging effects.
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- Purity: 99.76%
- CAS No.: 525-79-1
- 화학식: C10H9N5O
- 분자량:215.21
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Publications Citing Use of MedChemExpress (MCE) Kinetin
More- Nat Commun. 2025 Apr 11;16(1):3475. [Abstract]
- Nat Plants. 2024 Jan;10(1):180-191. [Abstract]
- Genes Dis. 2022 Sep 10;10(5):2151-2166. [Abstract]
- ACS Omega. 2024 Feb 28;9(10):11870-11882. [Abstract]
- Toxicol Appl Pharmacol. 2023 Sep 15:475:116655. [Abstract]
- ChemRxiv. 2025 Apr 11.
- Research Square Preprint. 2021 Aug.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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RT-PCR
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Cell Migration/Invasion Assay
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Apoptosis Analysis
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| HL-60 | IC50 |
21.9 μM
Compound: kinetin
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Antiproliferative activity against human HL60 cells
Antiproliferative activity against human HL60 cells
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[PMID: 17300934] |
Kinetin (N6-furfuryladenine) shows to have a direct effect on superoxide dismutase activity in plants; prevent oxidation of unsaturated acids in plant membranes; slow down development and aging in insects, by reducing their fecundity and increasing the specific activity of catalase; and delay the onset of many age-related characteristics that appear in normal human skin fibroblasts undergoing aging in vitro. Kinetin (70-150 μM) markedly suppressed hydroxyl radical formation by about 41% and 76%, respectively[1].
The plant cytokinin kinetin dramatically increases exon 20 inclusion in RNA isolated from cultured familial dysautonomia (FD) cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Subjects received 23.5 mg/kg/d for 28 d. An increase in WT IKBKAP mRNA expression in leukocytes was noted after 8 d in six of eight individuals; after 28 d, the mean increase compared with baseline was significant[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ADP-induced acute pulmonary thrombosis 20-24 g mice (ICR strain)[1]
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Dosage:2, 4, 6 mg/kg
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Administration:Injection into the tail vein
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Result:Reduced mortality to 70%, 40% and 35% at 2, 4, and 6 mg/kg, respectively.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 525-79-1
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Appearance Solid
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분자량 215.21
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화학식 C10H9N5O
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Color Off-white to light yellow
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SMILES
C12=NC=NC(NCC3=CC=CO3)=C1N=CN2
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Synonyms
6-Furfuryladenine; N6-Furfuryladenine
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (7)
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Journal Impact Factor
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Most Recent
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Nat Commun
2025 Apr 11;16(1):3475. PMID: 40216803 -
Nat Plants
2024 Jan;10(1):180-191. PMID: 38172575 -
Genes Dis
PTEN-induced putative kinase 1 regulates mitochondrial quality control and is essential for the maturation of human induced pluripotent stem cell-derived cardiomyocytes. [Abstract]2022 Sep 10;10(5):2151-2166. PMID: 37492732 -
ACS Omega
Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer. [Abstract]2024 Feb 28;9(10):11870-11882. PMID: 38496939 -
Toxicol Appl Pharmacol
Kinetin inhibits hepatic stellate cell activation and induces apoptosis via interactions with the TGF-β1/Smad signaling pathway. [Abstract]2023 Sep 15:475:116655. PMID: 37579951
Kinetin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2023 Sep 15:475:116655. [Abstract]
The viability of LX-2 cells was quantified with the CCK-8 assay following treatment with Kinetin (0, 5, 10, 20, 40, 60, 80 μg/mL).
Kinetin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2023 Sep 15:475:116655. [Abstract]
LX-2 cells were treated with TGF-β1 (10 ng/ml) and the noted concentrations of kinetin for 24 h or with 40 μg/mL Kinetin for the noted times, and the levels of expression of α-SMA and collagen I were quantified by Western blotting.
Kinetin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2023 Sep 15:475:116655. [Abstract]
LX-2 cells were treated with 40 μg/mL Kinetin for 48 h with or without 10 ng/ml TGF-β1 for 24 h, and the relative mRNA expression levels of α-SMA, collagen I, collagen III, PDGFR, Vimentin and Desmin were determined by qRT-PCR.
Kinetin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2023 Sep 15:475:116655. [Abstract]
LX-2 cells were treated with 40 μg/mL Kinetin for 48 h with or without 10 ng/ml TGF-β1 for 24 h, and cell migration was detected with wound healing assays.
Kinetin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2023 Sep 15:475:116655. [Abstract]
LX-2 cells were treated with 40 μg/mL Kinetin for 48 h with or without 10 ng/ml TGF-β1 for 24 h, and apoptosis was assessed by flow cytometry.
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용액&용해도
DMSO : 33.33 mg/mL (154.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
1M NaOH : 33.33 mg/mL (154.87 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Hsiao G, et al. Inhibitory activity of kinetin on free radical formation of activated platelets in vitro and on thrombus formation in vivo. Eur J Pharmacol. 2003 Apr 4;465(3):281-7. [Content Brief]
[2]. Hims MM, et al. Therapeutic potential and mechanism of kinetin as a treatment for the human splicing disease familial dysautonomia. J Mol Med (Berl). 2007 Feb;85(2):149-61. [Content Brief]
[3]. Axelrod FB, et al. Kinetin improves IKBKAP mRNA splicing in patients with familial dysautonomia. Pediatr Res. 2011 Nov;70(5):480-3. [Content Brief]
[4]. Griffaut B, et al. Cytotoxic effects of kinetin riboside on mouse, human and plant tumour cells. Int J Biol Macromol. 2004 Aug;34(4):271-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / 1M NaOH | 1 mM | 4.6466 mL | 23.2331 mL | 46.4662 mL | 116.1656 mL |
| 5 mM | 0.9293 mL | 4.6466 mL | 9.2932 mL | 23.2331 mL | |
| 10 mM | 0.4647 mL | 2.3233 mL | 4.6466 mL | 11.6166 mL | |
| 15 mM | 0.3098 mL | 1.5489 mL | 3.0977 mL | 7.7444 mL | |
| 20 mM | 0.2323 mL | 1.1617 mL | 2.3233 mL | 5.8083 mL | |
| 25 mM | 0.1859 mL | 0.9293 mL | 1.8586 mL | 4.6466 mL | |
| 30 mM | 0.1549 mL | 0.7744 mL | 1.5489 mL | 3.8722 mL | |
| 40 mM | 0.1162 mL | 0.5808 mL | 1.1617 mL | 2.9041 mL | |
| 50 mM | 0.0929 mL | 0.4647 mL | 0.9293 mL | 2.3233 mL | |
| 60 mM | 0.0774 mL | 0.3872 mL | 0.7744 mL | 1.9361 mL | |
| 80 mM | 0.0581 mL | 0.2904 mL | 0.5808 mL | 1.4521 mL | |
| 100 mM | 0.0465 mL | 0.2323 mL | 0.4647 mL | 1.1617 mL |
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