1. Autophagy
    Metabolic Enzyme/Protease
  2. Autophagy
    Cathepsin
  3. LV-320

LV-320 

Cat. No.: HY-112711 Purity: >95.0%
Handling Instructions

LV-320 is a potent and uncompetitive ATG4B inhibitor with an IC50 of 24.5 µM and a Kd of 16 µM. LV-320 inhibits ATG4B enzymatic activity, blocks autophagic flux in cells, and is stable, non-toxic and active in vivo.

For research use only. We do not sell to patients.

LV-320 Chemical Structure

LV-320 Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 515 In-stock
Estimated Time of Arrival: December 31
5 mg USD 450 In-stock
Estimated Time of Arrival: December 31
10 mg USD 750 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2250 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

LV-320 is a potent and uncompetitive ATG4B inhibitor with an IC50 of 24.5 µM and a Kd of 16 µM. LV-320 inhibits ATG4B enzymatic activity, blocks autophagic flux in cells, and is stable, non-toxic and active in vivo[1].

IC50 & Target

IC50: 24.5 µM (ATG4B); Kd: 16 μM (ATG4B)[1]

In Vitro

LV-320 (0-120 µM; SKBR3, MCF7, JIMT1, and MDA-MB-231 cells) treatment results in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines[1].
LV-320 (120 µM; 48 hours; MDA-MB-231 cells) treatment results in an increase in LC3B-II, indicating that LV-320 blocks autophagic flux[1].

Western Blot Analysis[1]

Cell Line: SKBR3, MCF7, JIMT1, and MDA-MB-231 cells
Concentration: 0 µM, 25 µM, 50 µM, 75 µM, 100 µM, or 120 µM
Incubation Time:
Result: Resulted in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines.

Cell Autophagy Assay[1]

Cell Line: MDA-MB-231 cells
Concentration: 120 µM
Incubation Time: 48 hours
Result: Blocked autophagic flux.
In Vivo

LV-320 (100-200 mg/kg; oral gavage; three times over two days; GFP-LC3 mice) treatment results in a terminal blood level of 169 µM and a liver level of 104 µM. The expression of GFP-LC3 puncta is significantly greater accumulation in LV-320 treated animals compared to controls. LC3B-II protein is also increased in LV-320-treated animals. The treatment do not cause significant toxicity in mice at either dose[1].

Animal Model: GFP-LC3 mice (females, 9-14 weeks)[1]
Dosage: 100 mg/kg or 200 mg/kg
Administration: Oral gavage; three times over two days (Pharmacokinetic study)
Result: Terminal blood levels were 169 µM and liver levels were 104 µM. LC3B-II protein level was also increased.
Molecular Weight

520.11

Formula

C₂₉H₂₆ClNO₂S₂

SMILES

ClC1=CC2=NC=CC(/C=C/C3=CC=C(C(SCCC(O)=O)SCCC4=CC=CC=C4)C=C3)=C2C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 135 mg/mL (259.56 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9227 mL 9.6134 mL 19.2267 mL
5 mM 0.3845 mL 1.9227 mL 3.8453 mL
10 mM 0.1923 mL 0.9613 mL 1.9227 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.25 mg/mL (4.33 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.25 mg/mL (4.33 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

LV-320LV320LV 320AutophagyCathepsinInhibitorinhibitorinhibit

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Product name:
LV-320
Cat. No.:
HY-112711
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