LV-320
Based on 1 Customer Validation
LV-320 is a potent and uncompetitive ATG4B inhibitor with an IC50 of 24.5 μM and a Kd of 16 μM. LV-320 inhibits ATG4B enzymatic activity, blocks autophagic flux in cells, and is stable, non-toxic and active in vivo.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 95.0%
- No. CAS: 2449093-46-1
- Fòrmula: C29H26ClNO2S2
- Peso molecular:520.11
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
IC50: 24.5 μM (ATG4B); Kd: 16 μM (ATG4B)[1]
LV-320 (0-120 μM; SKBR3, MCF7, JIMT1, and MDA-MB-231 cells) treatment results in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines[1].
LV-320 (120 μM; 48 hours; MDA-MB-231 cells) treatment results in an increase in LC3B-II, indicating that LV-320 blocks autophagic flux[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SKBR3, MCF7, JIMT1, and MDA-MB-231 cells
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Concentration:0 µM, 25 µM, 50 µM, 75 µM, 100 µM, or 120 µM
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Incubation Time:
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Result:Resulted in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines.
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Cell Line:MDA-MB-231 cells
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Concentration:120 µM
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Incubation Time:48 hours
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Result:Blocked autophagic flux.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:GFP-LC3 mice (females, 9-14 weeks)[1]
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Dosage:100 mg/kg or 200 mg/kg
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Administration:Oral gavage; three times over two days (Pharmacokinetic study)
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Result:Terminal blood levels were 169 µM and liver levels were 104 µM. LC3B-II protein level was also increased.
Chemical Information
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No. CAS 2449093-46-1
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Appearance Solid
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Peso molecular 520.11
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Fòrmula C29H26ClNO2S2
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Color Light yellow to yellow
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SMILES
ClC1=CC2=NC=CC(/C=C/C3=CC=C(C(SCCC(O)=O)SCCC4=CC=CC=C4)C=C3)=C2C=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
DMSO : 135 mg/mL (259.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.25 mg/mL (4.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9227 mL | 9.6134 mL | 19.2267 mL | 48.0668 mL |
| 5 mM | 0.3845 mL | 1.9227 mL | 3.8453 mL | 9.6134 mL | |
| 10 mM | 0.1923 mL | 0.9613 mL | 1.9227 mL | 4.8067 mL | |
| 15 mM | 0.1282 mL | 0.6409 mL | 1.2818 mL | 3.2045 mL | |
| 20 mM | 0.0961 mL | 0.4807 mL | 0.9613 mL | 2.4033 mL | |
| 25 mM | 0.0769 mL | 0.3845 mL | 0.7691 mL | 1.9227 mL | |
| 30 mM | 0.0641 mL | 0.3204 mL | 0.6409 mL | 1.6022 mL | |
| 40 mM | 0.0481 mL | 0.2403 mL | 0.4807 mL | 1.2017 mL | |
| 50 mM | 0.0385 mL | 0.1923 mL | 0.3845 mL | 0.9613 mL | |
| 60 mM | 0.0320 mL | 0.1602 mL | 0.3204 mL | 0.8011 mL | |
| 80 mM | 0.0240 mL | 0.1202 mL | 0.2403 mL | 0.6008 mL | |
| 100 mM | 0.0192 mL | 0.0961 mL | 0.1923 mL | 0.4807 mL |
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