1. Cell Cycle/DNA Damage
    Cytoskeleton
    Apoptosis
  2. Kinesin
    Apoptosis
  3. Monastrol

Monastrol (Synonyms: (±)-Monastrol)

Cat. No.: HY-101071A Purity: 99.95%
Handling Instructions

Monastrol is a potent and cell-permeable inhibitor of the mitotic kinesin Eg5 with an IC50 value of 14 μM.

For research use only. We do not sell to patients.

Monastrol Chemical Structure

Monastrol Chemical Structure

CAS No. : 329689-23-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 177 In-stock
Estimated Time of Arrival: December 31
1 mg USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 110 In-stock
Estimated Time of Arrival: December 31
25 mg USD 230 In-stock
Estimated Time of Arrival: December 31
50 mg USD 390 In-stock
Estimated Time of Arrival: December 31
100 mg USD 700 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

Monastrol is a potent and cell-permeable inhibitor of the mitotic kinesin Eg5 with an IC50 value of 14 μM.

IC50 & Target[3]

Eg5

14 μM (IC50)

In Vitro

Monastrol is a small, cell-permeable molecule that arrests cells in mitosis by specifically inhibiting Eg5, a member of the Kinesin-5 family. Monastrol treatment of dividing cells results in spindle collapse and cell cycle arrest with a monoastral spindle, which is similar to the phenotype observed when Eg5 is inhibited by anti-Eg5 antibodies[1]. Monastrol is an allosteric inhibitor of the mitotic kinesin Eg5 that exhibits an antiproliferative effect against several cell lines. Monastrol treatment can decrease cell viability in MCF-7 tumor cells. Real-time cell growth kinetic analysis showed a decrease in the proliferation of MCF-7 cells exposed to monastrol[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

292.35

Formula

C₁₄H₁₆N₂O₃S

CAS No.

329689-23-8

SMILES

O=C(C1=C(C)NC(NC1C2=CC=CC(O)=C2)=S)OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 33 mg/mL (112.88 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4206 mL 17.1028 mL 34.2056 mL
5 mM 0.6841 mL 3.4206 mL 6.8411 mL
10 mM 0.3421 mL 1.7103 mL 3.4206 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

The cytotoxicity assay is performed with MTT. Cells are seeded in 96-well culture plates (5000 cells/well) and incubated for 24 h for stabilization. After this period, the following treatments are administered for 24 and 48 h: vehicle control (0.5 % DMSO); 1 μM doxorubicin and monastrol at 5, 25, 50, 75, and 100 μM. After each time of treatment, the medium is withdrawn, serum-free media containing 0.5 mg/mL MTT salt is added and incubated for 4 h, and formazan crystal products are diluted[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

Monastrol(±)-MonastrolKinesinApoptosisInhibitorinhibitorinhibit

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Product Name:
Monastrol
Cat. No.:
HY-101071A
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