S-2474

Experiments are principally performed in the two conditions as follows. (i) Neurons (2.5×10⁵ cells/cm²) are treated with 10 μM Aβ(25-35) or Aβ(1-40) in the presence or absence of S-2474 at 37°C. Vehicle controls are treated with culture medium containing 1% deionized water and 0.1% DMSO. Aβ controls are treated with culture medium containing 10 μM Aβ(25-35) and 0.1% DMSO. (ii) Neurons (2.5×10⁵ cells/cm²) are treated with eicosanoids at 37°C. Vehicle controls are treated with culture medium containing 0.1% ethanol. Two different methods are employed for assessment of neurotoxicity of Aβ. First, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide dye (MTT) reduction assay reflecting mitochondrial succinate dehydrogenase activity is employed. Second, residual cells are counted according to morphologic criteria; neurons with intact neurites and a smooth, round soma are considered viable, whereas those with degenerated neurites and an irregular soma are considered nonviable.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.