1. Stem Cell/Wnt
    TGF-beta/Smad
  2. TGF-beta/Smad

SIS3 

Cat. No.: HY-13013 Purity: >98.0%
Handling Instructions

SIS3 is a potent and selective inhibitor of Smad3. a potent regulator of the human Nox4 promoter.

For research use only. We do not sell to patients.

SIS3 Chemical Structure

SIS3 Chemical Structure

CAS No. : 521984-48-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 151 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
2 mg USD 80 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
5 mg USD 140 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 210 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
25 mg USD 420 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

    SIS3 purchased from MCE. Usage Cited in: Int J Clin Exp Med. 2016;9(8):15892-15899.

    CNE1 cells are cultured with 3 μM SIS3 for 4 hs, and then washed by cold PBS and followed by the 10 ng/mL TGF-β treatments for 48 hs, respectively.

    SIS3 purchased from MCE. Usage Cited in: Int J Clin Exp Med. 2016;9(8):15892-15899.

    The protein expressions of EGFR are detected by Western blot in CNE1 after 5 nM AEE788 treatment for 4 hs, compared with the control.

    SIS3 purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Jun 21;9:660.

    By Western blotting, the α-SMA and palladin proteins are determined in cells that are pretreated with NF-κB (JSH-23), Wnt/β-catenin (XAV939), EGFR (erlotinib), p38 MAPK (TAK-715), and Smad3 (SIS3) inhibitors and are followed by TWEAK stimulation.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    SIS3 is a potent and selective inhibitor of Smad3. a potent regulator of the human Nox4 promoter. target: Smad3 SIS3 Reduces the TGF-β1-Dependent Increase in Phosphorylation and DNA Binding of Smad3. SIS3 has no effect on Smad2, p38 MAPK, ERK or PI 3-kinase signaling. SIS3 can block excessive ECM production from the TGF-β1-treated normal fibroblasts and scleroderma fibroblasts, the model of cells with autocrine TGF-β signaling in vitro.

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (61.23 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0409 mL 10.2043 mL 20.4086 mL
    5 mM 0.4082 mL 2.0409 mL 4.0817 mL
    10 mM 0.2041 mL 1.0204 mL 2.0409 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Molecular Weight

    489.99

    Formula

    C₂₈H₂₈ClN₃O₃

    CAS No.

    521984-48-5

    SMILES

    CN1C2=NC=CC=C2C(/C=C/C(N3CCC(C=C(OC)C(OC)=C4)=C4C3)=O)=C1C5=CC=CC=C5.[H]Cl

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: >98.0%

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
    C1   V1   C2   V2

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    Product Name:
    SIS3
    Cat. No.:
    HY-13013
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    SIS3

    Cat. No.: HY-13013