1. Stem Cell/Wnt
    TGF-beta/Smad
  2. TGF-beta/Smad

SIS3 

Cat. No.: HY-13013 Purity: >98.0%
Handling Instructions

SIS3 is a cell-permeable and selective inhibitor of Smad3. It inhibits Smad3 phosphorylation with an IC50 of 3 µM.

For research use only. We do not sell to patients.

SIS3 Chemical Structure

SIS3 Chemical Structure

CAS No. : 521984-48-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 151 In-stock
Estimated Time of Arrival: December 31
2 mg USD 80 In-stock
Estimated Time of Arrival: December 31
5 mg USD 140 In-stock
Estimated Time of Arrival: December 31
10 mg USD 210 In-stock
Estimated Time of Arrival: December 31
25 mg USD 420 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

    SIS3 purchased from MCE. Usage Cited in: Int J Clin Exp Med. 2016;9(8):15892-15899.

    CNE1 cells are cultured with 3 μM SIS3 for 4 hs, and then washed by cold PBS and followed by the 10 ng/mL TGF-β treatments for 48 hs, respectively.

    SIS3 purchased from MCE. Usage Cited in: Int J Clin Exp Med. 2016;9(8):15892-15899.

    The protein expressions of EGFR are detected by Western blot in CNE1 after 5 nM AEE788 treatment for 4 hs, compared with the control.

    SIS3 purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Jun 21;9:660.

    By Western blotting, the α-SMA and palladin proteins are determined in cells that are pretreated with NF-κB (JSH-23), Wnt/β-catenin (XAV939), EGFR (erlotinib), p38 MAPK (TAK-715), and Smad3 (SIS3) inhibitors and are followed by TWEAK stimulation.

    SIS3 purchased from MCE. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3824-3836.

    NRK-49F cells are treated with TGF-β1 for the indicated time. The levels of p-STAT3, p-AKT, p-GSK3α, and p-GSK3β expression are detected in comparison with the levels of total STAT3, AKT, GSK3α, GSK3β, and with GAPDH as the loading control.

    SIS3 purchased from MCE. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3824-3836.

    LY2109761 at 100 nM, 10 μM, or 50 μM is added for 30 min to block TGFβR1/2 prior to TGF-β1-treatment, then proteins are collected, and the levels of Sphk2, fibronectin, and α-SMA are detected by Western blotting.

    SIS3 purchased from MCE. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3824-3836.

    NRK-49F cells are preincubated with 100 nM, 10 μM, or 50 μM Smad3 inhibitor SIS3 for 30 min followed by TGF-β1 (2 ng/mL) stimulation for 12 h. Then, the expression of Sphk2, fibronectin, and α-SMA is determined by Western blotting.

    SIS3 purchased from MCE. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3824-3836.

    NRK-49F cells are pretreated with Fyn inhibitor SU6656 (1 μM) for 30 min, followed by TGF-β1 stimulation for 12 h, and then protein levels of fibronectin and α-SMA are determined by Western blotting.

    SIS3 purchased from MCE. Usage Cited in: Mol Immunol. 2018 Oct 2;103:173-181.

    TRPM7 mRNA and protein expressions are effectively suppressed by LY2109761 and SIS3 in ASMCs compared with those in 10 ng/mL TGF-β1-stimulated cells.
    • Biological Activity

    • Technical Information

    • Purity & Documentation

    • References

    Description

    SIS3 is a cell-permeable and selective inhibitor of Smad3. It inhibits Smad3 phosphorylation with an IC50 of 3 µM.

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (61.23 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0409 mL 10.2043 mL 20.4086 mL
    5 mM 0.4082 mL 2.0409 mL 4.0817 mL
    10 mM 0.2041 mL 1.0204 mL 2.0409 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Molecular Weight

    489.99

    Formula

    C₂₈H₂₈ClN₃O₃

    CAS No.

    521984-48-5

    SMILES

    CN1C2=NC=CC=C2C(/C=C/C(N3CCC(C=C(OC)C(OC)=C4)=C4C3)=O)=C1C5=CC=CC=C5.[H]Cl

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: >98.0%

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    Product Name:
    SIS3
    Cat. No.:
    HY-13013
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    SIS3

    Cat. No.: HY-13013