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  2. E1/E2/E3 Enzyme
  3. Skp2 Inhibitor C1

Skp2 Inhibitor C1  (Synonyms: SKPin C1)

Cat. No.: HY-16661 Purity: 98.21%
COA Handling Instructions

Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis.

For research use only. We do not sell to patients.

Skp2 Inhibitor C1 Chemical Structure

Skp2 Inhibitor C1 Chemical Structure

CAS No. : 432001-69-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 107 In-stock
Solution
10 mM * 1 mL in DMSO USD 107 In-stock
Solid
5 mg USD 105 In-stock
10 mg USD 168 In-stock
50 mg USD 706 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Skp2 Inhibitor C1 purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2016 Jul;36(1):559-66.  [Abstract]

    Western blotting is performed to detect the expression of p21, p27 in PC-3-TxR cells and DU145-TxR cells treated with Paclitaxel or Skp2 inhibitors alone or their combination treatment. Paclitaxel or Skp2 inhibitor treatment alone leads to the accumulation of p27 in PC3-TxR or DU145-TxR.

    Skp2 Inhibitor C1 purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2016 Oct;14(4):3917-24.  [Abstract]

    BGC 823 human gastric cancer cells (3×105 cells/well) are plated into 48 well plates. After 6 h, the cells are treated with SKPin C1 at concentrations of 1, 5 and 10 μM for 48 h. The expression levels of Skp2 and p27kip1 are detected using western blot analysis and grey values are calcu-lated.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis[1].

    In Vitro

    Skp2 Inhibitor C1 (10-50 μM; 12 hr) decreases the viability of THP-1, U266 and RPMI 8226 cells[1].
    Skp2 Inhibitor C1 (25 μM) increases p27 protein levels in U266 and RPMI 8226 cells by inhibiting ubiquitination[1].
    Skp2 Inhibitor C1 (25 μM) inhibits cell cycle of U266 and RPMI 8226 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Cycle Analysis[1]

    Cell Line: B lymphocytes, THP-1, U266 and RPMI 8226 cells
    Concentration: 0, 5, 10, 25, and 50 μM
    Incubation Time: 12 hr, 24 hr, 36 hr, and 48 hr
    Result: Significantly decreased the viability of U266 and RPMI 8226 cells at 10 μM for 12 hours.
    Did not significantly affect B lymphocyte viability at 50 μM.
    Decreased THP-1 cell viability at 50 μM for 12 hours.

    Cell Viability Assay[1]

    Cell Line: U266 and RPMI 8226 cells
    Concentration: 0, 5, 10, 25, and 50 μM
    Incubation Time: 12 hr
    Result: Increased the percentages of U266 and RPMI 8226 cells in the G0/G1 phase, while decreased the percentages in S and G2/M phases.
    In Vivo

    Skp2 Inhibitor C1 (5 mg/kg and 10 mg/kg; 3 times within 24 h: 24, 5, and 1 h before the test) shows the antidepressant-like effect in mouse models following chronic treatment by using the tail suspension test (TST), forced swimming test (FST), and social interaction test (SIT)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    465.34

    Formula

    C18H13BrN2O4S2

    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    S=C(S/1)N(CC2=CN=CC=C2)C(C1=C\C(C=C(Br)C=C3)=C3OCC(O)=O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 20.83 mg/mL (44.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1490 mL 10.7448 mL 21.4897 mL
    5 mM 0.4298 mL 2.1490 mL 4.2979 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
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    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.21%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1490 mL 10.7448 mL 21.4897 mL 53.7242 mL
    5 mM 0.4298 mL 2.1490 mL 4.2979 mL 10.7448 mL
    10 mM 0.2149 mL 1.0745 mL 2.1490 mL 5.3724 mL
    15 mM 0.1433 mL 0.7163 mL 1.4326 mL 3.5816 mL
    20 mM 0.1074 mL 0.5372 mL 1.0745 mL 2.6862 mL
    25 mM 0.0860 mL 0.4298 mL 0.8596 mL 2.1490 mL
    30 mM 0.0716 mL 0.3582 mL 0.7163 mL 1.7908 mL
    40 mM 0.0537 mL 0.2686 mL 0.5372 mL 1.3431 mL
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    Skp2 Inhibitor C1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Skp2 Inhibitor C1
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