1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme
  3. Skp2 Inhibitor C1

Skp2 Inhibitor C1  (Synonyms: SKPin C1)

Cat. No.: HY-16661 Purity: 98.21%
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Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis.

For research use only. We do not sell to patients.

Skp2 Inhibitor C1 Chemical Structure

Skp2 Inhibitor C1 Chemical Structure

CAS No. : 432001-69-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 185 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 185 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 168 In-stock
Estimated Time of Arrival: December 31
50 mg USD 706 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Skp2 Inhibitor C1 purchased from MCE. Usage Cited in: Oncol Rep. 2016 Jul;36(1):559-66.  [Abstract]

    Western blotting is performed to detect the expression of p21, p27 in PC-3-TxR cells and DU145-TxR cells treated with Paclitaxel or Skp2 inhibitors alone or their combination treatment. Paclitaxel or Skp2 inhibitor treatment alone leads to the accumulation of p27 in PC3-TxR or DU145-TxR.

    Skp2 Inhibitor C1 purchased from MCE. Usage Cited in: Mol Med Rep. 2016 Oct;14(4):3917-24.  [Abstract]

    BGC 823 human gastric cancer cells (3×105 cells/well) are plated into 48 well plates. After 6 h, the cells are treated with SKPin C1 at concentrations of 1, 5 and 10 μM for 48 h. The expression levels of Skp2 and p27kip1 are detected using western blot analysis and grey values are calcu-lated.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis[1].

    In Vitro

    Skp2 Inhibitor C1 (10-50 μM; 12 hr) decreases the viability of THP-1, U266 and RPMI 8226 cells[1].
    Skp2 Inhibitor C1 (25 μM) increases p27 protein levels in U266 and RPMI 8226 cells by inhibiting ubiquitination[1].
    Skp2 Inhibitor C1 (25 μM) inhibits cell cycle of U266 and RPMI 8226 cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Cycle Analysis[1]

    Cell Line: B lymphocytes, THP-1, U266 and RPMI 8226 cells
    Concentration: 0, 5, 10, 25, and 50 μM
    Incubation Time: 12 hr, 24 hr, 36 hr, and 48 hr
    Result: Significantly decreased the viability of U266 and RPMI 8226 cells at 10 μM for 12 hours.
    Did not significantly affect B lymphocyte viability at 50 μM.
    Decreased THP-1 cell viability at 50 μM for 12 hours.

    Cell Viability Assay[1]

    Cell Line: U266 and RPMI 8226 cells
    Concentration: 0, 5, 10, 25, and 50 μM
    Incubation Time: 12 hr
    Result: Increased the percentages of U266 and RPMI 8226 cells in the G0/G1 phase, while decreased the percentages in S and G2/M phases.
    In Vivo

    Skp2 Inhibitor C1 (5 mg/kg and 10 mg/kg; 3 times within 24 h: 24, 5, and 1 h before the test) shows the antidepressant-like effect in mouse models following chronic treatment by using the tail suspension test (TST), forced swimming test (FST), and social interaction test (SIT)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    465.34

    Formula

    C18H13BrN2O4S2

    CAS No.
    SMILES

    S=C(S/1)N(CC2=CN=CC=C2)C(C1=C\C(C=C(Br)C=C3)=C3OCC(O)=O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 20.83 mg/mL (44.76 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1490 mL 10.7448 mL 21.4897 mL
    5 mM 0.4298 mL 2.1490 mL 4.2979 mL
    10 mM 0.2149 mL 1.0745 mL 2.1490 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 98.21%

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Skp2 Inhibitor C1
    Cat. No.:
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