1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme

DKM 2-93 

Cat. No.: HY-101836 Purity: 98.87%
Handling Instructions

DKM 2-93 is a relatively selective inhibitor of UBA5 with an IC50 of 430 μM.

For research use only. We do not sell to patients.

DKM 2-93 Chemical Structure

DKM 2-93 Chemical Structure

CAS No. : 65836-72-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 92 In-stock
Estimated Time of Arrival: December 31
100 mg USD 84 In-stock
Estimated Time of Arrival: December 31
200 mg USD 144 In-stock
Estimated Time of Arrival: December 31
500 mg USD 288 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Description

DKM 2-93 is a relatively selective inhibitor of UBA5 with an IC50 of 430 μM.

IC50 & Target

IC50: 430 μM (UBA5), 90 μM (PaCa2 cells), 30 μM (Panc1 cells)[1]

In Vitro

Ubiquitin-like modifier activating enzyme 5 (UBA5) is a novel pancreatic cancer therapeutic target. DKM 2-93 impairs pancreatic cancer cell survival through covalently modifying the catalytic cysteine of UBA5, thereby inhibiting its activity as a protein that activates the ubiquitin-like protein UFM1 to UFMylate proteins. DKM 2-93 inhibits PaCa2 and Panc1 cells survival with IC50s of 90 and 30 μM, respectively[1].

In Vivo

DKM 2-93 daily treatment significantly impairs tumor growth of PaCa2 cells in vivo in tumor xenograft studies in immune-deficient mice without causing any weight loss or overt toxicity[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (615.54 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1036 mL 20.5179 mL 41.0357 mL
5 mM 0.8207 mL 4.1036 mL 8.2071 mL
10 mM 0.4104 mL 2.0518 mL 4.1036 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

PaCa2 and Panc1 cells are treated with 0-1000 μM DKM 2-93 for 48 hours. Cell viability is assessed by Hoescht stain[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice: Mice are subcutaneously injected with PaCa2 cells to initiate the tumor xenograft study and treatments of mice are initiated with vehicle or DKM 2-93 (50 mg/kg ip, once per day) three days after injection of cancer cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

243.69

Formula

C₁₁H₁₄ClNO₃

CAS No.

65836-72-8

SMILES

O=C(NCC1=CC=C(OC)C(OC)=C1)CCl

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
DKM 2-93
Cat. No.:
HY-101836
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DKM 2-93

Cat. No.: HY-101836