2. Apoptosis Metabolic Enzyme/Protease
  3. MDM-2/p53 Apoptosis Endogenous Metabolite
  4. C16-Ceramide (d18:1/16:0)

C16-Ceramide (d18:1/16:0)  (Synonyms: C16-Ceramide)

Cat. No.: HY-100354 Purity: 99.92%
COA
SDS
Handling Instructions Technical Support

C16-Ceramide (d18:1/16:0) is a natural small molecule activating p53 through the direct and selective binding.

For research use only. We do not sell to patients.

CAS No. : 24696-26-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in Ethanol In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of C16-Ceramide (d18:1/16:0):

C16-Ceramide (d18:1/16:0) Related Antibodies

Top Publications Citing Use of Products

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Dec 1:19:10645-10660.  [Abstract]

    The relative mRNA levels of TFRC and ACSL4 in SH-SY5Y cells treated with C16-Ceramide (50 μM).

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Dec 1:19:10645-10660.  [Abstract]

    Western blot analysis of C16-Ceramide (50 μM) confirmed that activation of p53 weakened the inhibitory effect of EMD on ferroptosis.

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Lipids Health Dis. 2025 Apr 12;24(1):138.  [Abstract]

    Western blot analysis of the expression of P53, SHISA5, type I collagen, MMP2, p-IRE1α, GRP78, p-JNK, P62 and LC3-II proteins in HCF cells after activation of P53 by C16-ceramide (10 µM, 24 h).

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Jul 25;17(14):13358-13376.  [Abstract]

    C16-Ceramide (5 μM/10 μM). Cell viability assessed by live (green)/dead (red) staining.

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Dec 1:522:105-118.  [Abstract]

    Cell proliferation experiments were conducted in PANC-1 shNT, shCerS6+EV, shCerS6+rWT, and shCerS6+rR131A cell lines with or without different doses of C16-ceramide, and in CFPAC-1 EV, WT, or R131A cell lines with or without different doses of cell-permeable C16-ceramide (PC16: 0.5 μM/12.5 μM).

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    C16-Ceramide (d18:1/16:0) is a natural small molecule activating p53 through the direct and selective binding[1].

    IC50 & Target

    p53[1], Apoptosis[2]
    In Vitro

    C16-Ceramide (d18:1/16:0) interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide (d18:1/16:0) in the cell[1].
    C16-Ceramide (d18:1/16:0) (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2].
    C16-Ceramide (d18:1/16:0) (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2].
    C16-Ceramide (d18:1/16:0) (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-Ceramide (d18:1/16:0) down-regulates Mdm2 expression via Btf[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    C16-Ceramide (d18:1/16:0) Related Antibodies

    Cell Viability Assay[2]

    Cell Line: HCT116 cells
    Concentration: 2.5, 5, 10, 12, 20, 50 µM
    Incubation Time: 0-48 h
    Result: Strongly decreased cell viability in a time- and concentration-dependent manner.

    Western Blot Analysis[2]

    Cell Line: HCT116 cells
    Concentration: 12 μM
    Incubation Time: 1, 3 and 6 h
    Result: Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion also partially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.
    Molecular Weight

    537.90

    Formula

    C34H67NO3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCCCCCCCCCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMF : 20 mg/mL (37.18 mM; ultrasonic and warming and heat to 60°C)

    Ethanol : 10 mg/mL (18.59 mM; ultrasonic and warming and heat to 60°C)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8591 mL 9.2954 mL 18.5908 mL
    5 mM 0.3718 mL 1.8591 mL 3.7182 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    90% Corn Oil

      Solubility: ≥ 2 mg/mL (3.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% HPMC/1% Tween-80 in Saline water

      Solubility: 4.95 mg/mL (9.20 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  15% Solutol HS 15    10% Cremophor EL    35% PEG400    40% Water

      Solubility: 5 mg/mL (9.30 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.92%

    SDS (393 KB)

    COA (238 KB) HNMR (264 KB) RP-HPLC (231 KB) MS (77 KB) Elemental Analysis Report (96 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMF 1 mM 1.8591 mL 9.2954 mL 18.5908 mL 46.4770 mL
    5 mM 0.3718 mL 1.8591 mL 3.7182 mL 9.2954 mL
    10 mM 0.1859 mL 0.9295 mL 1.8591 mL 4.6477 mL
    15 mM 0.1239 mL 0.6197 mL 1.2394 mL 3.0985 mL
    DMF 20 mM 0.0930 mL 0.4648 mL 0.9295 mL 2.3239 mL
    25 mM 0.0744 mL 0.3718 mL 0.7436 mL 1.8591 mL
    30 mM 0.0620 mL 0.3098 mL 0.6197 mL 1.5492 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    C16-Ceramide (d18:1/16:0) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    C16-Ceramide (d18:1/16:0)24696-26-2C16-CeramideMDM-2/p53ApoptosisEndogenous MetaboliteHCT116BtfBcl-2-associated transcription factorBAXInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    C16-Ceramide (d18:1/16:0)
    Cat. No.:
    HY-100354
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.