C16-Ceramide (d18:1/16:0)
Based on 7 publication(s) in Google Scholar
C16-Ceramide (d18:1/16:0) is a natural small molecule activating p53 through the direct and selective binding.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 24696-26-2
- Formula: C34H67NO3
- Molecular Weight:537.90
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Publications Citing Use of MedChemExpress (MCE) C16-Ceramide (d18:1/16:0)
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RT-PCR
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WB
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
All Endogenous Metabolite Isoforms
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Biological Activity
C16-Ceramide (d18:1/16:0) interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide (d18:1/16:0) in the cell[1].
C16-Ceramide (d18:1/16:0) (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2].
C16-Ceramide (d18:1/16:0) (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2].
C16-Ceramide (d18:1/16:0) (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-Ceramide (d18:1/16:0) down-regulates Mdm2 expression via Btf[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 cells
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Concentration:2.5, 5, 10, 12, 20, 50 µM
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Incubation Time:0-48 h
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Result:Strongly decreased cell viability in a time- and concentration-dependent manner.
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Cell Line:HCT116 cells
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Concentration:12 μM
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Incubation Time:1, 3 and 6 h
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Result:Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion also partially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.
Chemical Information
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CAS No. 24696-26-2
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Appearance Solid
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Molecular Weight 537.90
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Formula C34H67NO3
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Color White to off-white
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SMILES
CCCCCCCCCCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O
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Synonyms
C16-Ceramide
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (7)
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Journal Impact Factor
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Most Recent
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ACS Nano
Cell-Free Osteoarthritis Treatment with Sustained-Release of Chondrocyte-Targeting Exosomes from Umbilical Cord-Derived Mesenchymal Stem Cells to Rejuvenate Aging Chondrocytes. [Abstract]2023 Jul 25;17(14):13358-13376. PMID: 37439514
C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Jul 25;17(14):13358-13376. [Abstract]
C16-Ceramide (5 μM/10 μM). Cell viability assessed by live (green)/dead (red) staining.
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Cancer Lett
AKT1/FOXP3 axis-mediated expression of CerS6 promotes p53 mutant pancreatic tumorigenesis. [Abstract]2021 Dec 1:522:105-118. PMID: 34343636
C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Dec 1:522:105-118. [Abstract]
Cell proliferation experiments were conducted in PANC-1 shNT, shCerS6+EV, shCerS6+rWT, and shCerS6+rR131A cell lines with or without different doses of C16-ceramide, and in CFPAC-1 EV, WT, or R131A cell lines with or without different doses of cell-permeable C16-ceramide (PC16: 0.5 μM/12.5 μM).
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Drug Des Devel Ther
Exploratory Study on the Role of Emodin in Alleviating MPTP-Induced Neurotoxicity: A Focus on p53-Ferroptosis Signaling. [Abstract]2025 Dec 1:19:10645-10660. PMID: 41356603
C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Dec 1:19:10645-10660. [Abstract]
The relative mRNA levels of TFRC and ACSL4 in SH-SY5Y cells treated with C16-Ceramide (50 μM).
C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Dec 1:19:10645-10660. [Abstract]
Western blot analysis of C16-Ceramide (50 μM) confirmed that activation of p53 weakened the inhibitory effect of EMD on ferroptosis.
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Lipids Health Dis
2025 Apr 12;24(1):138. PMID: 40221727
C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Lipids Health Dis. 2025 Apr 12;24(1):138. [Abstract]
Western blot analysis of the expression of P53, SHISA5, type I collagen, MMP2, p-IRE1α, GRP78, p-JNK, P62 and LC3-II proteins in HCF cells after activation of P53 by C16-ceramide (10 µM, 24 h).
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Solvent & Solubility
DMF : 20 mg/mL (37.18 mM; ultrasonic and warming and heat to 60°C)
Ethanol : 10 mg/mL (18.59 mM; ultrasonic and warming and heat to 60°C)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2 mg/mL (3.72 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 0.5% HPMC/1% Tween-80 in Saline water
Solubility: 4.95 mg/mL (9.20 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 15% Solutol HS 15 10% Cremophor EL 35% PEG400 40% Water
Solubility: 5 mg/mL (9.30 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (269 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
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- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Fekry B, et al. C16-ceramide is a natural regulatory ligand of p53 in cellular stress response. Nat Commun. 2018 Oct 8;9(1):4149. [Content Brief]
[2]. Rénert AF, et al. The proapoptotic C16-ceramide-dependent pathway requires the death-promoting factor Btf in colon adenocarcinoma cells. J Proteome Res. 2009 Oct;8(10):4810-22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMF | 1 mM | 1.8591 mL | 9.2954 mL | 18.5908 mL | 46.4770 mL |
| 5 mM | 0.3718 mL | 1.8591 mL | 3.7182 mL | 9.2954 mL | |
| 10 mM | 0.1859 mL | 0.9295 mL | 1.8591 mL | 4.6477 mL | |
| 15 mM | 0.1239 mL | 0.6197 mL | 1.2394 mL | 3.0985 mL | |
| DMF | 20 mM | 0.0930 mL | 0.4648 mL | 0.9295 mL | 2.3239 mL |
| 25 mM | 0.0744 mL | 0.3718 mL | 0.7436 mL | 1.8591 mL | |
| 30 mM | 0.0620 mL | 0.3098 mL | 0.6197 mL | 1.5492 mL |
C16-Ceramide (d18:1/16:0) Related Classifications
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