C6 Ceramide (Synonyms: C6-Cer; N-Hexanoylsphingosine)

Name: C6 Ceramide
Brand: MedChemExpress
SKU: HY-19542
Rating: 5.0 (3 reviews)

Cat. No.: HY-19542 Purity: 99.82%: Data Sheet
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C6 Ceramide (C6-Cer) is a short-chain, cell-permeable ceramide pathway activator with anticancer activity. C6 Ceramide-mediated miR-29b expression participates in the progression of multiple myeloma through suppressing the proliferation, migration and angiogenesis of endothelial cells by targeting Akt signal pathway. C6 Ceramide exhibits multiple anti-cancer properties including cell cycle arrest, Apoptosis, inhibition of tumor growth and enhances the effects of chemotherapy in drug-resistant cancer cells. C6-ceramide can be used as an adjuvant for chemotherapeutic agents, to enhance anti-tumor effects.

For research use only. We do not sell to patients.

CAS No. : 124753-97-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of C6 Ceramide:

Ceramide C6-d₇ In-stock
C6 Ceramide-d₁₁ In-stock
N-Hexanoyl-L-erythro-sphingosine Get quote
C6 Ceramide-¹³C₂,d₂ Get quote

3 Publications Citing Use of MCE C6 Ceramide

ELISA

Cell Proliferation/Viability Assay

Cell Imaging/Staining

Bio/Physico-chemical Assay

: C6 Ceramide purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2024 Oct;14(10):4360-4377.; IL-27 supernatant concentration of BMDMs stimulated by UDP and IMQ after PKC, JNK, p38, and ERK agonist (C6 Ceramide, 10 μmol/L) treatment.

: C6 Ceramide purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 May 1:923:171405. [Abstract]; Cell viability of BRL-3A cells after C6 Ceramide (0-50 μM) intervention for 24 h was detected by CCK-8. Other groups were compared with 0 μM group.

: C6 Ceramide purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 May 1:923:171405. [Abstract]; The expression levels of apoptosis-related proteins in BRL-3A cells after 25 μM C6 Ceramide intervention and/or 20 μM cadmium exposure for 24 h were detected by western blotting.

: C6 Ceramide purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 May 1:923:171405. [Abstract]; C6 Ceramide (25 μM). Hoechst/PI double staining: Hoechst/PI was used to double stain and observe them under fluorescence microscope (200×). Hoechst 33342 can stain the nucleus with blue fluorescence. PI can stain necrotic cells with red fluorescence.

: C6 Ceramide purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 May 1:923:171405. [Abstract]; C6 Ceramide (25 μM). The levels of oxidative stress related indicators GSH, MDA, and SOD in BRL-3A cells.

: C6 Ceramide purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 May 1:923:171405. [Abstract]; C6 Ceramide (25 μM). ROS production was detected by DCFH-DA staining (200×).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	35 μM Compound: C6-Cer	Cytotoxicity against human A549 cells after 72 hrs by MTT test Cytotoxicity against human A549 cells after 72 hrs by MTT test	[PMID: 17081760]
MCF7	IC₅₀	12 μM Compound: C6Cer	Antiproliferative activity against human MCF7 cells after 24 hrs by trypan blue exclusion assay Antiproliferative activity against human MCF7 cells after 24 hrs by trypan blue exclusion assay	[PMID: 20851613]
SUP-T1	IC₅₀	48.01 μM Compound: C6 ceramide	Cytotoxicity against human SupT1 cells after 24 hrs by CellTiter-Blue assay Cytotoxicity against human SupT1 cells after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]

In Vitro

C6 Ceramide (10 μg/mL, 48 h) enhances Trichostatin A (TSA) (HY-15144)-induced cell death, cell Apoptosis and induces a-tubulin acetylation in both CaOV3 and L3.6 cancer cells^[1].
C6 Ceramide (10 μg/mL, 12 h) disrupts HDAC6/PP1/tubulin complex, leading to the release of PP1 and AKT dephosphorylation and inhibition in CaOV3 and L3.6 cancer cells ^[1].
C6 Ceramide (10 μM, 48 h) increases the expression of miR29b and decreases the expressions of Akt3, PI3K and VEGFA in HUVECs^[2].
C6 Ceramide (3 and 10 μM, 7 days) increases the number of viable hippocampal neurons and enhances the dendritic outgrowth of neurons in vitro^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	CaOV3 and L3.6 cancer cells
Concentration:	10 μg/mL
Incubation Time:	48 h
Result:	Had almost no effect on cancer cell death alone, but markedly enhanced TSA-induced cell death in a dosedependent manner in both CaOV3 and L3.6 cells.

Western Blot Analysis^[2]

Cell Line:	HUVECs
Concentration:	10 μM
Incubation Time:	48 h
Result:	Signifcantly increased the expression of miR29b and decreased the expressions of Akt3, PI3K and VEGFA in HUVECs.

Molecular Weight

397.63

Formula

C₂₄H₄₇NO₃

CAS No.

124753-97-5

Appearance

Solid

Color

White to off-white

SMILES

CCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (251.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5149 mL	12.5745 mL	25.1490 mL
5 mM	0.5030 mL	2.5149 mL	5.0298 mL
10 mM	0.2515 mL	1.2575 mL	2.5149 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (240 KB) HNMR (143 KB) RP-HPLC (235 KB) LCMS (233 KB)

Handling Instructions (2659 KB)

References

[1]. Zhu Q, et, al. C6-ceramide synergistically potentiates the anti-tumor effects of histone deacetylase inhibitors via AKT dephosphorylation and α-tubulin hyperacetylation both in vitro and in vivo. Cell Death Dis. 2011 Jan 27;2(1):e117. [Content Brief]

[2]. Liu L, et, al. C6-ceramide treatment inhibits the proangiogenic activity of multiple myeloma exosomes via the miR-29b/Akt pathway. J Transl Med. 2020 Aug 3;18(1):298. [Content Brief]

[3]. Mitoma J, et al. Bipotential roles of ceramide in the growth of hippocampal neurons: promotion of cell survival and dendritic outgrowth in dose- and developmental stage-dependent manners. J Neurosci Res. 1998 Mar 15;51(6):712-22. [Content Brief]

[4]. Flowers M, et al. C6-ceramide and targeted inhibition of acid ceramidase induce synergistic decreases in breast cancer cell growth. Breast Cancer Res Treat. 2012 Jun;133(2):447-58. [Content Brief]

[5]. Wilhelm R, et al. C6 Ceramide (d18:1/6:0) as a Novel Treatment of Cutaneous T Cell Lymphoma. Cancers (Basel). 2021 Jan 13;13(2):270. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5149 mL	12.5745 mL	25.1490 mL	62.8725 mL
	5 mM	0.5030 mL	2.5149 mL	5.0298 mL	12.5745 mL
	10 mM	0.2515 mL	1.2575 mL	2.5149 mL	6.2873 mL
	15 mM	0.1677 mL	0.8383 mL	1.6766 mL	4.1915 mL
	20 mM	0.1257 mL	0.6287 mL	1.2575 mL	3.1436 mL
	25 mM	0.1006 mL	0.5030 mL	1.0060 mL	2.5149 mL
	30 mM	0.0838 mL	0.4192 mL	0.8383 mL	2.0958 mL
	40 mM	0.0629 mL	0.3144 mL	0.6287 mL	1.5718 mL
	50 mM	0.0503 mL	0.2515 mL	0.5030 mL	1.2575 mL
	60 mM	0.0419 mL	0.2096 mL	0.4192 mL	1.0479 mL
	80 mM	0.0314 mL	0.1572 mL	0.3144 mL	0.7859 mL
	100 mM	0.0251 mL	0.1257 mL	0.2515 mL	0.6287 mL

C6 Ceramide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C6 Ceramide124753-97-5C6-Cer N-HexanoylsphingosineApoptosisceramide pathwayanticancer activitymiR-29bAkt signal pathwaytumor growthInhibitorinhibitorinhibit

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C6 Ceramide (Synonyms: C6-Cer; N-Hexanoylsphingosine)

Customer Review

Other Forms of C6 Ceramide:

C6 Ceramide Related Antibodies

3 Publications Citing Use of MCE C6 Ceramide

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•Related Screening Libraries:

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Biological Activity

Purity & Documentation

References

Customer Review

C6 Ceramide Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

C6 Ceramide Related Classifications

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