CEMT 

CEMT is a carboxylesterase (CE) substrate and a ratiometric two-photon fluorescent reporter probe. CEMT can be hydrolyzed by CE to generate HMT, which is used for mitochondrial pH sensing. After activation by CE, CEMT exhibits ratiometric fluorescence changes in response to pH variations. CEMT targets and covalently binds to mitochondria, and can avoid leakage during acidification, thus enabling in situ imaging^[1].

CEMT (10 μM) exhibits a linear ratiometric fluorescence response to carboxylesterase concentrations from 0 to 15 μg/mL in cell-free 40 mM B-R buffer (pH 7.42) at 37 °C, and its CE-mediated hydrolysis product HMT exhibits a linear ratiometric fluorescence response to pH values from 4.98 to 9.07^[1].
CEMT (10 μM; 20 min) intracellular hydrolysis product HMT exhibits strong mitochondrial colocalization in HepG2 cells, with minimal leakage even under acidic (pH 4.98) conditions, confirming CEMT's ability to lock to mitochondria for in situ imaging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

482.36

C₂₆H₂₁Cl₂NO₄

2413816-64-3

CC(OC1=CC=C2C=C(C(OC2=C1)=O)/C=C/C3=CC=[N+](C=C3)CC4=CC=C(C=C4)CCl)=O.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Jiang A , et al. Ratiometric two-photon fluorescent probe for in situ imaging of carboxylesterase (CE)-mediated mitochondrial acidification during medication. Chem Commun (Camb). 2019;55(76):11358-11361.  [Content Brief]