Art. -Nr.: HY-N6002

3'-Hydroxypterostilbene

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

3'-Hydroxypterostilbene is a Pterostilbene (HY-N0828) analogue. 3'-Hydroxypterostilbene inhibits the growth of COLO 205, HCT-116 and HT-29 cells with IC₅₀s of 9.0, 40.2 and 70.9 μM, respectively. 3'-Hydroxypterostilbene significantly down-regulates PI3K/Akt and MAPKs signaling pathways and effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene can be used for the research of cancer.

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Reinheit: 99.89%
CAS. Nr.: 475231-21-1
Formel: C₁₆H₁₆O₄
Molecular Weight:272.30

Speicherung:

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

3'-Hydroxypterostilbene Chemical Structure

CAS. Nr.: 475231-21-1

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 9.0 μM (COLO 205),40.2 μM (HCT-116), 70.9 μM (HT-29)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	0.8 μM Compound: 12d	In vitro inhibitory concentration against proliferation of HL60 cells In vitro inhibitory concentration against proliferation of HL60 cells	[PMID: 12877593]

In Vitro

3'-Hydroxypterostilbene (5-50 μM; 24 h) affects cell proliferation of COLO 205, HCT-116 and HT-29^[1].
3'-Hydroxypterostilbene (5-100 μM; 24 h) induces cell apoptosis and the concentration of ROS plays an important role as an early mediator in 3'-Hydroxypterostilbene-induces apoptosis^[1].
3'-Hydroxypterostilbene (5-50 μM; 24 h) induces caspase-9 cleavage, increases caspase-3 activity and inhibits the mTOR/p70S6K, PI3K/Akt and MAPKs signaling pathways in COLO 205 cells^[1].
3'-Hydroxypterostilbene (25-50 μM; 24 h) induces autophagy in COLO 205 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

COLO 205, HCT-116 and HT-29 cell lines
Concentration:

5, 10, 25 and 50 μM
Incubation Time:

24 hours
Result:

Inhibited cell proliferation of COLO 205, HCT-116 and HT-29 cells with IC₅₀s of 9.0, 40.2 and 70.9 μM, respectively.

Apoptosis Analysis^[1]

Cell Line:

COLO 205 cells
Concentration:

5-100 μM
Incubation Time:

24 hours
Result:

Induced apoptosis in human colorectal carcinoma cells.

Western Blot Analysis^[1]

Cell Line:

COLO 205 cells
Concentration:

5-50 μM
Incubation Time:

24 hours
Result:

Degraded 116 kDa PARP into 85 kDa fragments and induced DFF-45 protein degradation and increased the amounts of LC3B I/II proteins than pterostilbene. Markedly decreased phosphorylation of mTOR, p70S6K (Thr389), PI3K, Akt and p38 MAPK in cells.

Cell Autophagy Assay^[1]

Cell Line:

COLO 205 cells
Concentration:

25 and 50 μM
Incubation Time:

24 hours
Result:

Showed an inducing effect on autophagy than pterostilbene in COLO 205 cells.

In Vivo

3'-Hydroxypterostilbene (10 mg/kg; i.p. once per day for 15 days) strongly inhibits tumor growth in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

3-4 week old BALB/c nude mice with human colorectal carcinoma COLO 205 tumor xenografts^[1]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection; 10 mg/kg once per day for 15 days
Result:

Significantly inhibited tumor volume and tumor weight after 15 days treatment compared with the pterostilbene-treated mice, and decreased the protein levels of COX-2, MMP-9, VEGF, cyclin D1 and pro-caspase-3 in mice.

Chemical Information

CAS. Nr. 475231-21-1
Appearance Solid
Molecular Weight 272.30
Formel C₁₆H₁₆O₄
Color Off-white to gray
SMILES

COC1=CC(/C=C/C2=CC=C(O)C(O)=C2)=CC(OC)=C1
Structure Classification
- Phenols
- Polyphenols
- Stilbenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Publications (1)

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Biomed Chromatogr

High-resolution mass spectrometry-based methodology for the identification of the metabolites of pterostilbene produced by rat, dog and human hepatocytes. [Abstract]

2021 Sep;35(9):e5138. PMID: 33830523

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (459.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6724 mL

18.3621 mL

36.7242 mL

5 mM

0.7345 mL

3.6724 mL

7.3448 mL

10 mM

0.3672 mL

1.8362 mL

3.6724 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Cheng TC, et al. Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors. PLoS One. 2014 Nov 12;9(11):e111814. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6724 mL	18.3621 mL	36.7242 mL	91.8105 mL
	5 mM	0.7345 mL	3.6724 mL	7.3448 mL	18.3621 mL
	10 mM	0.3672 mL	1.8362 mL	3.6724 mL	9.1811 mL
	15 mM	0.2448 mL	1.2241 mL	2.4483 mL	6.1207 mL
	20 mM	0.1836 mL	0.9181 mL	1.8362 mL	4.5905 mL
	25 mM	0.1469 mL	0.7345 mL	1.4690 mL	3.6724 mL
	30 mM	0.1224 mL	0.6121 mL	1.2241 mL	3.0604 mL
	40 mM	0.0918 mL	0.4591 mL	0.9181 mL	2.2953 mL
	50 mM	0.0734 mL	0.3672 mL	0.7345 mL	1.8362 mL
	60 mM	0.0612 mL	0.3060 mL	0.6121 mL	1.5302 mL
	80 mM	0.0459 mL	0.2295 mL	0.4591 mL	1.1476 mL
	100 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9181 mL

Keywords

3'-Hydroxypterostilbene

1 Cited Publications

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3'-Hydroxypterostilbene

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Publications Citing Use of MedChemExpress (MCE) 3'-Hydroxypterostilbene

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

3'-Hydroxypterostilbene

Phenols Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cell Death Library

Bioactive Compound Library Max

Natural Product Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Publications Citing Use of MedChemExpress (MCE) 3'-Hydroxypterostilbene

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

3'-Hydroxypterostilbene Related Classifications

HY-N6002 Related Classifications