5-Ethynyl-2'-deoxyuridine
Based on 37 publication(s) in Google Scholar
5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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- Pureté: 99.26%
- CAS No.: 61135-33-9
- Formule: C11H12N2O5
- Masse moléculaire:252.23
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-Ethynyl-2'-deoxyuridine
More- Nat Genet. 2025 Dec;57(12):3050-3065. [Abstract]
- Nat Aging. 2025 Mar;5(3):401-418. [Abstract]
- Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. [Abstract]
- Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
- Nat Commun. 2025 Nov 4;16(1):9664. [Abstract]
- J Am Chem Soc. 2026 Mar 11;148(9):9478-9493. [Abstract]
- J Clin Invest. 2023 Apr 3;133(7):e162324. [Abstract]
- NPJ Biofilms Microbiomes. 2026 Jan 8;12(1):38. [Abstract]
- J Cachexia Sarcopenia Muscle. 2024 Aug;15(4):1388-1403. [Abstract]
- Int J Biol Macromol. 2025 Jun;315(Pt 1):144297. [Abstract]
- NPJ Parkinsons Dis. 2026 May 26. [Abstract]
- Cell Rep. 2025 Nov 25;44(11):116496. [Abstract]
- Br J Pharmacol. 2025 Nov;182(21):5409-5429. [Abstract]
- PLoS Biol. 2022 Apr 27;20(4):e3001619 [Abstract]
- Neurosci Bull. 2025 May 2. [Abstract]
- JCI Insight. 2024 Dec 20;9(24):e183889. [Abstract]
- Inflamm Res. 2026 Jan 14;75(1):19. [Abstract]
- Front Endocrinol. 2023 Jan 9:13:1052487. [Abstract]
- Exp Neurol. 2026 May:399:115667. [Abstract]
- J Cell Mol Med. 2026 Mar 23;30(6):e71099.
- Reprod Biomed Online. 2022 May;44(5):803-816. [Abstract]
- Kaohsiung J Med Sci. 2022 Apr;38(4):357-366. [Abstract]
- Reprod Sci. 2022 Aug;29(8):2414-2427. [Abstract]
- Cell Microbiol. 2021 Dec;23(12):e13397. [Abstract]
- Nutr Res Pract. 2023 Dec;17(6):1070-1083. [Abstract]
- J Chemother. 2022 Oct;34(6):401-413. [Abstract]
- Lett Drug Des Discov. 2026 May 5.
- bioRxiv. 2026 Jun 14.
- bioRxiv. 2026 May 26.
- bioRxiv. 2026 May 29.
- NAR Mol Med. 2026 Jan 23;3(1):ugag005. [Abstract]
- bioRxiv. 2025 Aug 1:2025.07.31.667081. [Abstract]
- SSRN. 2025 May 29.
- bioRxiv. 2025 Apr 28:2025.04.28.651040. [Abstract]
- Res Sq. 2024 Jun 03.
- Res Sq. 2024 May 20.
- bioRxiv. 2023 Jun 22.
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Histological Imaging/Staining
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Histological Imaging/Staining
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Histological Imaging/Staining
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Histological Imaging/Staining
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Histological Imaging/Staining
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Activité biologique
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Alkyl-Chain |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| E6SM | EC50 |
0.1 μg/mL
Compound: 7, EDU
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Antiviral activity against Vaccinia virus in E6SM cells assessed as reduction of virus-induced cytopathogenicity
Antiviral activity against Vaccinia virus in E6SM cells assessed as reduction of virus-induced cytopathogenicity
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[PMID: 17518459] |
| E6SM | EC50 |
0.7 μg/mL
Compound: 7, EDU
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Antiviral activity against HSV1 KOS in E6SM cells assessed as reduction of virus-induced cytopathogenicity
Antiviral activity against HSV1 KOS in E6SM cells assessed as reduction of virus-induced cytopathogenicity
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[PMID: 17518459] |
| E6SM | EC50 |
0.7 μg/mL
Compound: 7, EDU
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Antiviral activity against thymidine kinase-deficient HSV1 B2006 in E6SM cells assessed as reduction of virus-induced cytopathogenicity
Antiviral activity against thymidine kinase-deficient HSV1 B2006 in E6SM cells assessed as reduction of virus-induced cytopathogenicity
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[PMID: 17518459] |
| E6SM | EC50 |
2 μg/mL
Compound: 7, EDU
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Antiviral activity against HSV2 G in E6SM cells assessed as reduction of virus-induced cytopathogenicity
Antiviral activity against HSV2 G in E6SM cells assessed as reduction of virus-induced cytopathogenicity
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[PMID: 17518459] |
| E6SM | EC50 |
4 μg/mL
Compound: 7, EDU
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Antiviral activity against thymidine kinase deficient HSV1 VMW1837 in E6SM cells assessed as reduction of virus-induced cytopathogenicity
Antiviral activity against thymidine kinase deficient HSV1 VMW1837 in E6SM cells assessed as reduction of virus-induced cytopathogenicity
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[PMID: 17518459] |
| FM3A | IC50 |
>=250 μM
Compound: 7, EDU
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Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells
Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells
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[PMID: 17518459] |
| FM3A | IC50 |
0.13 μM
Compound: 7, EDU
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Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV1 thymidine kinase
Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV1 thymidine kinase
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[PMID: 17518459] |
| FM3A | IC50 |
0.39 μM
Compound: 7, EDU
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Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV2 thymidine kinase
Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV2 thymidine kinase
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[PMID: 17518459] |
| HEL | CC50 |
2.5 μM
Compound: 7, EDU
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Cytotoxicity against HEL cells
Cytotoxicity against HEL cells
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[PMID: 17518459] |
| HEL | EC50 |
0.11 μM
Compound: 7, EDU
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Antiviral activity against TK+ VZV YS in HEL cells assessed as reduction of virus plaque formation
Antiviral activity against TK+ VZV YS in HEL cells assessed as reduction of virus plaque formation
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[PMID: 17518459] |
| HEL | EC50 |
0.16 μM
Compound: 7, EDU
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Antiviral activity against TK+ VZV OKA in HEL cells assessed as reduction of virus plaque formation
Antiviral activity against TK+ VZV OKA in HEL cells assessed as reduction of virus plaque formation
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[PMID: 17518459] |
| HEL | EC50 |
0.75 μM
Compound: 7, EDU
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Antiviral activity against thymidine kinase deficient VZV YS in HEL cells assessed as reduction of virus plaque formation
Antiviral activity against thymidine kinase deficient VZV YS in HEL cells assessed as reduction of virus plaque formation
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[PMID: 17518459] |
| HEL | EC50 |
0.81 μM
Compound: 7, EDU
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Antiviral activity against thymidine kinase deficient VZV 07/1 in HEL cells assessed as reduction of virus plaque formation
Antiviral activity against thymidine kinase deficient VZV 07/1 in HEL cells assessed as reduction of virus plaque formation
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[PMID: 17518459] |
| HEL | EC50 |
0.85 μM
Compound: 7, EDU
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Antiviral activity against HCMV Davis in HEL cells assessed as reduction of virus plaque formation
Antiviral activity against HCMV Davis in HEL cells assessed as reduction of virus plaque formation
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[PMID: 17518459] |
| HEL | EC50 |
1.2 μM
Compound: 7, EDU
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Antiviral activity against HCMV AD169 in HEL cells assessed as reduction of virus plaque formation
Antiviral activity against HCMV AD169 in HEL cells assessed as reduction of virus plaque formation
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[PMID: 17518459] |
| MCF7 | IC50 |
0.4 μM
Compound: 2h
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Cytotoxicity against human MCF7 cells after 72 hrs
Cytotoxicity against human MCF7 cells after 72 hrs
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[PMID: 17336074] |
| MDA-MB-231 | IC50 |
4.4 μM
Compound: 2h
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Cytotoxicity against human MDA-MB-231 cells after 72 hrs
Cytotoxicity against human MDA-MB-231 cells after 72 hrs
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[PMID: 17336074] |
| OST | IC50 |
23 μM
Compound: 7, EDU
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Inhibition of proliferation of thymidine kinase deficient OST cells
Inhibition of proliferation of thymidine kinase deficient OST cells
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[PMID: 17518459] |
| S49 | EC50 |
0.12 μM
Compound: EdUrd
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Effective concentration to inhibit the growth of Wild-type S49 cells
Effective concentration to inhibit the growth of Wild-type S49 cells
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[PMID: 6796687] |
5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Two month old female mice[1]
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Dosage:10, 20, 50, 100 or 200 mg/kg
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Administration:Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
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Result:EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 61135-33-9
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Appearance Solid
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Masse moléculaire 252.23
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Formule C11H12N2O5
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SMILES
O[C@H]1C[C@H](N2C(NC(C(C#C)=C2)=O)=O)O[C@@H]1CO
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Synonyms
EdU
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (37)
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Journal Impact Factor
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Most Recent
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Nat Genet
TGF-β builds a dual immune barrier in colorectal cancer by impairing T cell recruitment and instructing immunosuppressive SPP1+ macrophages. [Abstract]2025 Dec;57(12):3050-3065. PMID: 41203813 -
Nat Aging
2025 Mar;5(3):401-418. PMID: 39794469 -
Cell Stem Cell
Hepatic cytochrome P450 8B1 and cholic acid potentiate intestinal epithelial injury in colitis by suppressing intestinal stem cell renewal. [Abstract]2022 Sep 1;29(9):1366-1381.e9. PMID: 36055192
5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. [Abstract]
Representative Edu and Olfm4 staining and quantitation in intestine of CA-treated colitic mice. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-2’-deoxyuridine (Edu) at 100 mg/kg was intraperitoneally injected into mice 2 h prior to killing.
5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. [Abstract]
Representative Muc2 or Lyz with Edu staining of intestine sections. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-2’-deoxyuridine (Edu) at 100 mg/kg was intraperitoneally injected into mice 2 h prior to killing.
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Cell Host Microbe
A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage. [Abstract]2024 Feb 14;32(2):191-208.e9. PMID: 38237593
5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
Ki67 and EdU staining of distal jejunum of mice treated. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-20-deoxyuridine at 100 mg/kg was intraperitoneally injected into mice 12 h prior to sacrifice. P. g: Live P. goldsteinii. Red/white dotted line marks single intestinal crypt. Ten random points of one sample were picked and the average value for each mouse was calculated as one single point. Red: EdU; Blue: DAPI. n=10 replicates/group.
5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
EdU (upper set) and TUNEL (bottom set) staining of organoids after being cocultured with HPG or LPG. White dotted line marks a single organoid. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-20-deoxyuridine at 100 mg/kg was intraperitoneally injected into mice 12 h prior to sacrifice. Scale bars: 20 μm. Red: EdU; Green: TUNEL; Blue: DAPI. n=20 biological replicates/group.
5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
Statistical analysis of EdU (Left) and TUNEL (right) staining of organoids generated from mice treated. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-20-deoxyuridine at 100 mg/kg was intraperitoneally injected into mice 12 h prior to sacrifice. n=20 biological replicates/group.
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Nat Commun
Regenerating liver uses ammonia to support de novo pyrimidine synthesis and cell proliferation. [Abstract]2025 Nov 4;16(1):9664. PMID: 41188238 -
J Am Chem Soc
2026 Mar 11;148(9):9478-9493. PMID: 41739600 -
J Clin Invest
Coordinated activation of c-Src and FOXM1 drives tumor cell proliferation and breast cancer progression. [Abstract]2023 Apr 3;133(7):e162324. PMID: 36795481 -
NPJ Biofilms Microbiomes
Disruption of bile acid homeostasis potentiates Paneth cell ablation by activating the intestinal Farnesoid X receptor in necrotizing enterocolitis. [Abstract]2026 Jan 8;12(1):38. PMID: 41507219 -
J Cachexia Sarcopenia Muscle
Single-cell RNA-seq reveals novel interaction between muscle satellite cells and fibro-adipogenic progenitors mediated with FGF7 signalling. [Abstract]2024 Aug;15(4):1388-1403. PMID: 38751367 -
Int J Biol Macromol
Ionized Angelica polysaccharides derivatives slow tumor growth in vivo and induce the apoptosis of HepG2 cells via mitochondria death receptor-mediated pathways in vitro. [Abstract]2025 Jun;315(Pt 1):144297. PMID: 40383338 -
NPJ Parkinsons Dis
Cryopreservable dopaminergic progenitors derived from human iPSCs with accelerated loss of pluripotency and early functional restoration in Parkinsonian rats. [Abstract]2026 May 26. PMID: 42191734 -
Cell Rep
A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential. [Abstract]2025 Nov 25;44(11):116496. PMID: 41166305 -
Br J Pharmacol
Cholinergic G protein-coupled bile acid receptor 1 (TGR5/GPBA) in the medial septal orchestrates adult hippocampal neurogenesis and cognition in Alzheimer's disease mice. [Abstract]2025 Nov;182(21):5409-5429. PMID: 40926398 -
PLoS Biol
Optineurin promotes myogenesis during muscle regeneration in mice by autophagic degradation of GSK3β. [Abstract]2022 Apr 27;20(4):e3001619 PMID: 35476671 -
Neurosci Bull
Endothelial Cell Integrin α6 Regulates Vascular Remodeling Through the PI3K/Akt-eNOS-VEGFA Axis After Stroke. [Abstract]2025 May 2. PMID: 40316875 -
JCI Insight
BPDCN MYB fusions regulate cell cycle genes, impair differentiation, and induce myeloid-dendritic cell leukemia. [Abstract]2024 Dec 20;9(24):e183889. PMID: 39499902 -
Inflamm Res
Erianin protects chondrocytes against IL-1β-induced oxidative stress and ferroptosis by activating GPX4/STING signaling in osteoarthritis. [Abstract]2026 Jan 14;75(1):19. PMID: 41533098 -
Front Endocrinol
Corticosterone induces obesity partly via promoting intestinal cell proliferation and survival. [Abstract]2023 Jan 9:13:1052487. PMID: 36699046 -
Exp Neurol
Exosomes-mediated delivery of miR-27a-3p antagomir alleviates white matter injury by regulating PPARγ/PRDX1/JNK pathway after subarachnoid hemorrhage in rats. [Abstract]2026 May:399:115667. PMID: 41581691 -
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Reprod Biomed Online
MicroRNA let-7i inhibits granulosa-luteal cell proliferation and oestradiol biosynthesis by directly targeting IMP2. [Abstract]2022 May;44(5):803-816. PMID: 35339367 -
Kaohsiung J Med Sci
PAX9 functions as a tumor suppressor gene for cervical cancer via modulating cell proliferation and apoptosis. [Abstract]2022 Apr;38(4):357-366. PMID: 34931758 -
Reprod Sci
Identification of Circular RNA circ_0017068 as a Regulator of Proliferation and Apoptosis in Trophoblast Cells by miR-330-5p/XIAP Axis. [Abstract]2022 Aug;29(8):2414-2427. PMID: 34981461 -
Cell Microbiol
Chlamydia and HPV induce centrosome amplification in the host cell through additive mechanisms. [Abstract]2021 Dec;23(12):e13397. PMID: 34716742 -
Nutr Res Pract
Sanghuangporus sanghuang extract inhibits the proliferation and invasion of lung cancer cells in vitro and in vivo. [Abstract]2023 Dec;17(6):1070-1083. PMID: 38053828 -
J Chemother
Circ_SETD3 regulates gefitinib sensitivity and tumor progression by miR-873-5p-dependent regulation of APPBP2 in non-small cell lung cancer. [Abstract]2022 Oct;34(6):401-413. PMID: 34861803 -
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NAR Mol Med
The fluoropyrimidine polymer CF10 synergizes with 5-ethynyl-2'-deoxyuridine by promoting telomere attrition and mitotic catastrophe. [Abstract]2026 Jan 23;3(1):ugag005. PMID: 41635782 -
bioRxiv
A small molecule inhibitor of NVL suppresses tumor growth by blocking ribosome biogenesis. [Abstract]2025 Aug 1:2025.07.31.667081. PMID: 40766499 -
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bioRxiv
2025 Apr 28:2025.04.28.651040. PMID: 40492193 -
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Solvant et solubilité
DMSO : 50 mg/mL (198.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (99.12 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 4 mg/mL (15.86 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Pureté et documentation
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Fiche technique (278 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Chenbo Zeng, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32. [Content Brief]
[2]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.9646 mL | 19.8232 mL | 39.6464 mL | 99.1159 mL |
| 5 mM | 0.7929 mL | 3.9646 mL | 7.9293 mL | 19.8232 mL | |
| 10 mM | 0.3965 mL | 1.9823 mL | 3.9646 mL | 9.9116 mL | |
| 15 mM | 0.2643 mL | 1.3215 mL | 2.6431 mL | 6.6077 mL | |
| 20 mM | 0.1982 mL | 0.9912 mL | 1.9823 mL | 4.9558 mL | |
| 25 mM | 0.1586 mL | 0.7929 mL | 1.5859 mL | 3.9646 mL | |
| 30 mM | 0.1322 mL | 0.6608 mL | 1.3215 mL | 3.3039 mL | |
| 40 mM | 0.0991 mL | 0.4956 mL | 0.9912 mL | 2.4779 mL | |
| 50 mM | 0.0793 mL | 0.3965 mL | 0.7929 mL | 1.9823 mL | |
| 60 mM | 0.0661 mL | 0.3304 mL | 0.6608 mL | 1.6519 mL | |
| 80 mM | 0.0496 mL | 0.2478 mL | 0.4956 mL | 1.2389 mL | |
| DMSO | 100 mM | 0.0396 mL | 0.1982 mL | 0.3965 mL | 0.9912 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5-Ethynyl-2'-deoxyuridine Related Classifications
HY-118411 Related Classifications
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