5-Ethynyluridine (Synonyms: 5-EU)

Name: 5-Ethynyluridine
Brand: MedChemExpress
SKU: HY-141140
Rating: 5.0 (2 reviews)

Cat. No.: HY-141140 Purity: 99.15%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

5-Ethynyluridine (5-EU) is a potent cell-permeable nucleoside can be used to label newly synthesized RNA. 5-Ethynyluridine can be used for isolation and sequencing of nascent RNA from neuronal populations in vivo. 5-Ethynyluridine can be used to identify changes in transcription in vivo in nervous system disease models. 5-Ethynyluridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

CAS No. : 69075-42-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 5-Ethynyluridine

RT-PCR

: 5-Ethynyluridine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 25;16(1):6880. [Abstract]; RT-qPCR detected the mature mRNA (across exon 2 and exon 3) and the newly transcribed nascent transcription (intron 3) of Tyms in RRP1 KO RAW 264.7 cells upon IL-1β stimulation or not. RAW 264.7 cells were pre-labeled with 5-Ethyluridine (EU) (250 μM; 5 min).

: 5-Ethynyluridine purchased from MedChemExpress. Usage Cited in: Sci Signal. 2024 Nov 26;17(864):eadp1375. [Abstract]; The cultured LNCaP cells were pulsed with 5-Ethynyluridine (5-EU) (1 mM; 1 h) to visualize nascent rRNA in the nucleolus as determined by co-localization with fibrillarin, and sharp decreases were observed upon GCN2iB treatment.

: 5-Ethynyluridine purchased from MedChemExpress. Usage Cited in: Sci Signal. 2024 Nov 26;17(864):eadp1375. [Abstract]; 22Rv1 WT and GCN2 KO cells were labeled with 5-Ethynyluridine (5-EU) (1 mM; 1 h) and co-stained with fibrillarin and Hoechst 33342.

: 5-Ethynyluridine purchased from MedChemExpress. Usage Cited in: Sci Signal. 2024 Nov 26;17(864):eadp1375. [Abstract]; DKO and TKO organoids were treated with vehicle or GCN2iB (5 μM) for 5 days and pulsed with 5-Ethynyluridine (5-EU) (1 mM; 1 h). The p53-null TKO organoids displayed the most robust reduction in 5-Ethynyluridine (5-EU) (1 mM; 1 h) signal as compared to the DKO organoids. 5-Ethynyluridine (5-EU) was labeled with Cy-5 using Click-iT, and organoids were stained with Hoechst 33342. The nucleolar 5-Ethynyluridine (5-EU) signal normalized to the organoid area (Hoechst 3342 signal) was quantified by immunofluorescence and is depicted in the bar granhs.

: 5-Ethynyluridine purchased from MedChemExpress. Usage Cited in: Sci Signal. 2024 Nov 26;17(864):eadp1375. [Abstract]; DKO and TKO organoids were separately treated with vehicle or GCN2iB (5 μM;5 d) with or without EAAs or adenine (50 μM) and pulsed with 5-Ethynyluridine (5-EU) (1 mM; 1 h). 5-Ethynyluridine (5-EU) was labeled with Cy-5 using Click-iT, and organoids were stained with Hoechst 33342. The nucleolar 5-Ethynyluridine (5-EU) signal normalized to the organoid area (Hoechst 3342 signal) was quantified by immunofluorescence and is depicted in the bar granhs.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Ethynyluridine (5-EU) is a potent cell-permeable nucleoside can be used to label newly synthesized RNA. 5-Ethynyluridine can be used for isolation and sequencing of nascent RNA from neuronal populations in vivo. 5-Ethynyluridine can be used to identify changes in transcription in vivo in nervous system disease models^[1]^[2]. 5-Ethynyluridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	CC₅₀	> 300 μg/mL Compound: 6	Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 3 days by XTT assay Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 3 days by XTT assay	[PMID: 27665179]

In Vivo

5-Ethynyluridine (75 mM in PBS for 1 μl; intracerebellar injection ) shows a labeling of central nervous system cells in mouse^[1].
5-Ethynyluridine (330-750 μMol/g; i.p.) results in in labelling of nascent RNA in cells of multiple organs and tissues, but not in the central nervous system in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

268.22

Formula

C₁₁H₁₂N₂O₆

CAS No.

69075-42-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(C(C#C)=C2)=O)=O)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (372.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (93.21 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7283 mL	18.6414 mL	37.2828 mL
5 mM	0.7457 mL	3.7283 mL	7.4566 mL
10 mM	0.3728 mL	1.8641 mL	3.7283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.71 mg/mL (2.65 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.71 mg/mL (2.65 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.71 mg/mL (2.65 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.15%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (256 KB) HNMR (263 KB) RP-HPLC (238 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Van't Sant LJ, et al. In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol Commun. 2021 May 21;9(1):94. [Content Brief]

[2]. Jao CY, et al. Exploring RNA transcription and turnover in vivo by using click chemistry. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15779-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.7283 mL	18.6414 mL	37.2828 mL	93.2071 mL
	5 mM	0.7457 mL	3.7283 mL	7.4566 mL	18.6414 mL
	10 mM	0.3728 mL	1.8641 mL	3.7283 mL	9.3207 mL
	15 mM	0.2486 mL	1.2428 mL	2.4855 mL	6.2138 mL
	20 mM	0.1864 mL	0.9321 mL	1.8641 mL	4.6604 mL
	25 mM	0.1491 mL	0.7457 mL	1.4913 mL	3.7283 mL
	30 mM	0.1243 mL	0.6214 mL	1.2428 mL	3.1069 mL
	40 mM	0.0932 mL	0.4660 mL	0.9321 mL	2.3302 mL
	50 mM	0.0746 mL	0.3728 mL	0.7457 mL	1.8641 mL
	60 mM	0.0621 mL	0.3107 mL	0.6214 mL	1.5535 mL
	80 mM	0.0466 mL	0.2330 mL	0.4660 mL	1.1651 mL
DMSO	100 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

5-Ethynyluridine Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Ethynyluridine69075-42-95-EUBiochemical Assay Reagentsnascent RNApurkinje cellsRNA transcriptsneuronsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

5-Ethynyluridine (Synonyms: 5-EU)

Customer Review

5-Ethynyluridine Related Antibodies

2 Publications Citing Use of MCE 5-Ethynyluridine

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

5-Ethynyluridine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report