Cyclosporin H
Based on 4 publication(s) in Google Scholar
Cyclosporin H is a selective and potent inhibitor of FPR-1 (formyl peptide receptor 1). Cyclosporin H, a viral transduction enhancer, increases lentiviral transduction up to 10-fold in human cord blood-derived hematopoietic stem and progenitor cells (HSPCs). Cyclosporin H displays an additive effect when combined with Rapamycin (HY-10219) or Prostaglandin E2 (HY-101952). Cyclosporin H lacks immunosuppressant activity of Cyclosporin A.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 83602-39-5
- Formula: C62H111N11O12
- Molecular Weight:1202.61
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Infection Compound Library
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Publications Citing Use of MedChemExpress (MCE) Cyclosporin H
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Customer Validation & Images
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Histological Imaging/Staining
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Bio/Physico-chemical Assay
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ELISA
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Histological Imaging/Staining
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WB
Biological Activity
The cyclic undecapeptide, cyclosporin H, is a potent inhibitor of formyl-Met-Leu-Phe (FMLP)-induced superoxide anion (O2-) formation in human neutrophils. Cyclosporin H inhibits FMLP binding in HL-60 membranes with a Ki of 0.1 μM. Cyclosporin H inhibits activation by FMLP of high affinity GTPase (the enzymatic activity of alpha-subunits of heterotrimeric regulatory guanine nucleotide-binding proteins) in HL-60 membranes with a Ki of 0.79 μM. Cyclosporin H inhibits the stimulatory effects of FMLP on cytosolic Ca2+ concentration ([Ca2+]i), O2- formation, and beta-glucuronidase release with Ki values of 0.08, 0.24, and 0.45 μM, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 83602-39-5
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Appearance Solid
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Molecular Weight 1202.61
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Formula C62H111N11O12
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Color White to off-white
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Sequence
Cyclo[{Abu}-{Sar}-{N(Me)Leu}-Val-{N(Me)Leu}-Ala-{d-Ala}-{N(Me)Leu}-{N(Me)Leu}-{d-N(Me)Val}-{N(Me)Bmt(E)}]
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Sequence Shortening
Cyclo[{Abu}-{Sar}-{N(Me)Leu}-V-{N(Me)Leu}-A-{d-Ala}-{N(Me)Leu}-{N(Me)Leu}-{d-N(Me)Val}-{N(Me)Bmt(E)}]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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Cancer Res
DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma. [Abstract]2022 Aug 16;82(16):2887-2903. PMID: 35731019 -
JHEP Rep
2026 Apr 24;8(8):101869. PMID: 42035895 -
Res Rep Urol
Protective Effects of Cyclosporin H Against Sepsis-Induced Acute Kidney Injury via Modulation of Fpr1 Signaling and Inhibiting Pyroptosis. [Abstract]2026 Mar 7:18:578231. PMID: 41821570
Cyclosporin H purchased from MedChemExpress. Usage Cited in: Res Rep Urol. 2026 Mar 7:18:578231. [Abstract]
Cyclosporin H (Cyclosporin) (5 mg/kg; i.p.; single dose). Representative images of HE-stained kidney tissue (red arrows indicate normal glomeruli, green arrows indicate normal renal tubules, blue arrows indicate atrophic glomeruli, purple arrows also indicate vacuolar degeneration of glomeruli, yellow arrows indicate interstitial hemorrhage), bar=100 μm.
Cyclosporin H purchased from MedChemExpress. Usage Cited in: Res Rep Urol. 2026 Mar 7:18:578231. [Abstract]
Levels of blood urea nitrogen and creatinine in serum. The results showed that serum BUN and creatinine levels of were significantly elevated in the SA-AKI group compared with control, whereas in the Cyclosporin H (5 mg/kg; i.p.; single dose) intervention group they were significantly lower than in the SA-AKI group.
Cyclosporin H purchased from MedChemExpress. Usage Cited in: Res Rep Urol. 2026 Mar 7:18:578231. [Abstract]
Levels of TNF-α and IL-1β in serum and kidney tissue. ELISA showed that TNF-α and IL-1β levels in serum were markedly higher in SA-AKI group than controls and were reduced by Cyclosporin H (5 mg/kg; i.p.; single dose) treatment versus SA-AKI group.
Cyclosporin H purchased from MedChemExpress. Usage Cited in: Res Rep Urol. 2026 Mar 7:18:578231. [Abstract]
Cyclosporin H (5 mg/kg; i.p.; single dose). Representative images of TUNEL-stained kidney tissue (green indicates apoptotic renal epithelial cells, DAPI stains cell nuclei, Merge shows the combination of TUNEL and DAPI staining), bar=100 μm.
Cyclosporin H purchased from MedChemExpress. Usage Cited in: Res Rep Urol. 2026 Mar 7:18:578231. [Abstract]
Western blot analysis of ASC, Caspase-1, NLRP3, and IL-1β protein expression changes in kidney tissues from control, SA-AKI, and Cyclosporin H (5 mg/kg; i.p.; single dose) intervention groups.
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Solvent & Solubility
DMSO : 100 mg/mL (83.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3 mg/mL (2.49 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3 mg/mL (2.49 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 1.25 mg/mL (1.04 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang X, et al. Mitochondrial peptides cause proinflammatory responses in the alveolar epithelium via FPR-1, MAPKs, and AKT: a potential mechanism involved in acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2018;315(5):L775-L786. [Content Brief]
[2]. Wenzel-Seifert K, et al. Cyclosporin H is a potent and selective formyl peptide receptor antagonist. Comparison with N-t-butoxycarbonyl-L-phenylalanyl-L-leucyl-L-phenylalanyl-L- leucyl-L-phenylalanine and cyclosporins A, B, C, D, and E. J Immunol. 1993;150(10):4591-4599. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8315 mL | 4.1576 mL | 8.3152 mL | 20.7881 mL |
| 5 mM | 0.1663 mL | 0.8315 mL | 1.6630 mL | 4.1576 mL | |
| 10 mM | 0.0832 mL | 0.4158 mL | 0.8315 mL | 2.0788 mL | |
| 15 mM | 0.0554 mL | 0.2772 mL | 0.5543 mL | 1.3859 mL | |
| 20 mM | 0.0416 mL | 0.2079 mL | 0.4158 mL | 1.0394 mL | |
| 25 mM | 0.0333 mL | 0.1663 mL | 0.3326 mL | 0.8315 mL | |
| 30 mM | 0.0277 mL | 0.1386 mL | 0.2772 mL | 0.6929 mL | |
| 40 mM | 0.0208 mL | 0.1039 mL | 0.2079 mL | 0.5197 mL | |
| 50 mM | 0.0166 mL | 0.0832 mL | 0.1663 mL | 0.4158 mL | |
| 60 mM | 0.0139 mL | 0.0693 mL | 0.1386 mL | 0.3465 mL | |
| 80 mM | 0.0104 mL | 0.0520 mL | 0.1039 mL | 0.2599 mL |
Cyclosporin H Related Classifications
HY-P1122 Related Classifications
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