Cycotiamine
Cycotiamine is an S-acylated thiamine derivative with antiplasmodial activity. Cycotiamine inhibits the proliferation of Plasmodium falciparum and Plasmodium knowlesi. Cycotiamine can be used in malaria-related research.
For research use only. We do not sell to patients.
- CAS No.: 6092-18-8
- Formula: C13H16N4O3S
- Molecular Weight:308.36
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Cycotiamine (compound 12) exhibits IC50 values of 123 μM, 127 μM, and 176 μM against P. falciparum under extracellular thiamine concentrations of 0 μM, 2.97 μM, and 297 μM, respectively. The IC50 of cycotiamine against P. knowlesi exceeds 1000 μM, showing weaker anti-parasitic activity than other thiamine analogs in the same series. The IC50 of cycotiamine against human fibroblast HFF cells exceeds 1000 μM, with no obvious cytotoxicity[1].
Cycotiamine (serial dilution; 96 h) inhibits the proliferation of both Plasmodium falciparum strain 3D7-GFP and PfTPK-GFP-overexpressing Plasmodium to the same extent, indicating that its anti-Plasmodium effect is independent of thiamine pyrophosphokinase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 6092-18-8
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Molecular Weight 308.36
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Formula C13H16N4O3S
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SMILES
O=CN(CC1=CN=C(C)N=C1N)/C(C)=C2SC(OCC/2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cycotiamine Related Classifications
HY-W742094 Related Classifications
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