Doramapimod hydrochloride (Synonyms: BIRB 796 hydrochloride)

Name: Doramapimod hydrochloride
Brand: MedChemExpress
SKU: HY-10320A
Rating: 5.0 (51 reviews)

Cat. No.: HY-10320A Purity: 98.29%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Doramapimod hydrochloride (BIRB 796 hydrochloride) is an anti-inflammatory compound with biological activity through inhibition of p38 MAPK. Doramapimod hydrochloride can significantly inhibit the activities of TNF-α and IL-1β induced by LPS, LTA and PGN. Doramapimod hydrochloride showed a stronger inhibitory effect on inflammation induced by all three bacterial toxins, which was more significant compared with the effects of other compounds. Doramapimod hydrochloride can be used in the research of autoimmune diseases.

For research use only. We do not sell to patients.

CAS No. : 1283526-53-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 51 publication(s) in Google Scholar

Other Forms of Doramapimod hydrochloride:

Doramapimod In-stock

51 Publications Citing Use of MCE Doramapimod hydrochloride

2D/3D Cell Culture and Differentiation

: Doramapimod hydrochloride purchased from MedChemExpress. Usage Cited in: Engineering. 2025 Mar 4.; BIRB 796 (10 μM, 36 h) inhibited the expression of p-p38 MAPK induced by LPS (200 ng/mL) in THP-1 cells.

: Doramapimod hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 6:76:103344. [Abstract]; BIRB 796 (DORA, 10 μM, 4 h) inhibited the expression of p-p38 MAPK induced by LPS (1000 ng/mL) in 10T1/2 cells.

: Doramapimod hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 6:76:103344. [Abstract]; BIRB 796 (DORA, 10 μM, 4 h) inhibited the mitochondrial recruitment of DRP1 and the fission induced by LPS (1000 ng/mL) in 10T1/2 cells.

: Doramapimod hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Jul;30(7):574-589. [Abstract]; Birb 796 (0.1, 0.3, 1, and 3 μM, 3 days) impaired the ability of SSK1 (0.5 μM,3 days) to specifically kill senescent HUVEC.

: Doramapimod hydrochloride purchased from MedChemExpress. Usage Cited in: Nature. 2019 Jul;571(7763):127-131. [Abstract]; BIRB 796 (1 μM, 4.5 h) did not abrogate EGF-induced pUGDH in A549 cells stably expressing Flag–UGDH.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

564.12

Formula

C₃₁H₃₈ClN₅O₃

CAS No.

1283526-53-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(C(C)(C)C)=NN1C2=CC=C(C)C=C2)NC3=C4C=CC=CC4=C(OCCN5CCOCC5)C=C3.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (177.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7727 mL	8.8634 mL	17.7267 mL
5 mM	0.3545 mL	1.7727 mL	3.5453 mL
10 mM	0.1773 mL	0.8863 mL	1.7727 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.29%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (256 KB) HNMR (147 KB) RP-HPLC (248 KB) LCMS (234 KB)

Handling Instructions (2659 KB)

References

[1]. Dietrich J, et al. The design, synthesis, and evaluation of 8 hybrid DFG-out allosteric kinase inhibitors. Bioorg Med Chem. 2010 Aug 1;18(15):5738-48 [Content Brief]

[2]. Anti-inflammatory effects of a p38 MAP kinase inhibitor, doramapimod, against bacterial cell wall toxins in equine whole blood [Content Brief]

[3]. Cicenas J, et al. JNK, p38, ERK, and SGK1 Inhibitors in Cancer. Cancers (Basel). 2017 Dec 21;10(1). pii: E1. [Content Brief]

[4]. Kuma Y, et al. BIRB796 inhibits all p38 MAPK isoforms in vitro and in vivo. J Biol Chem, 2005, 280(20), 19472-19479. [Content Brief]

[5]. He D, et al. BIRB796, the inhibitor of p38 mitogen-activated protein kinase, enhances the efficacy of chemotherapeutic agents in ABCB1 overexpression cells. PLoS One. 2013;8(1):e54181. [Content Brief]

[6]. Park JK, et al. p38 mitogen-activated protein kinase inhibition ameliorates angiotensin II-induced target organ damage. Hypertension. 2007 Mar;49(3):481-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7727 mL	8.8634 mL	17.7267 mL	44.3168 mL
	5 mM	0.3545 mL	1.7727 mL	3.5453 mL	8.8634 mL
	10 mM	0.1773 mL	0.8863 mL	1.7727 mL	4.4317 mL
	15 mM	0.1182 mL	0.5909 mL	1.1818 mL	2.9545 mL
	20 mM	0.0886 mL	0.4432 mL	0.8863 mL	2.2158 mL
	25 mM	0.0709 mL	0.3545 mL	0.7091 mL	1.7727 mL
	30 mM	0.0591 mL	0.2954 mL	0.5909 mL	1.4772 mL
	40 mM	0.0443 mL	0.2216 mL	0.4432 mL	1.1079 mL
	50 mM	0.0355 mL	0.1773 mL	0.3545 mL	0.8863 mL
	60 mM	0.0295 mL	0.1477 mL	0.2954 mL	0.7386 mL
	80 mM	0.0222 mL	0.1108 mL	0.2216 mL	0.5540 mL
	100 mM	0.0177 mL	0.0886 mL	0.1773 mL	0.4432 mL

Doramapimod hydrochloride Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Doramapimod1283526-53-3BIRB 796BIRB796BIRB-796AutophagyRafp38 MAPKRaf kinasesInhibitorinhibitorinhibit

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Doramapimod hydrochloride (Synonyms: BIRB 796 hydrochloride)

Customer Review

Other Forms of Doramapimod hydrochloride:

Doramapimod hydrochloride Related Antibodies

51 Publications Citing Use of MCE Doramapimod hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Raf Isoform Specific Products:

View All p38 MAPK Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Doramapimod hydrochloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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