DuP-697

Name: DuP-697
Brand: MedChemExpress
SKU: HY-103387
Rating: 4.5 (1 reviews)

Cat. No.: HY-103387 Purity: 99.79%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DuP-697 is a member of the vicinal diaryl heterocycles and a potent, irreversible, selective and orally active COX-2 inhibitor (IC₅₀ of 10 nM and 800 nM for human COX-2 and COX-1, respectively). DuP-697 exerts antiproliferative (IC₅₀ of 42.8 nM), antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. DuP-697 inhibits prostaglandin synthesis and has anti-inflammatory, anticancer and antipyretic effects.

For research use only. We do not sell to patients.

DuP-697 Chemical Structure

CAS No. : 88149-94-4

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE DuP-697

•Cell. 2025 Oct 16;188(21):5878-5894.e18. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All COX Isoform Specific Products:

View All Isoforms

COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[3]

hCOX-2

10 nM (IC₅₀)

hCOX-1

800 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	0.002 μM Compound: DuP-697	In vitro inhibition of the production of PGE-2 in arachidonic acid stimulated chinese hamster ovary (CHO) cells transfected with human cyclooxygenase-2 In vitro inhibition of the production of PGE-2 in arachidonic acid stimulated chinese hamster ovary (CHO) cells transfected with human cyclooxygenase-2	10.1016/S0960-894X(96)00580-X
CHO	IC₅₀	0.002 μM Compound: DuP-697	Tested in vitro for the ability to inhibit Prostaglandin G/H synthase 2 in chinese hamster ovary (CHO) cells Tested in vitro for the ability to inhibit Prostaglandin G/H synthase 2 in chinese hamster ovary (CHO) cells	10.1016/S0960-894X(96)00582-3
CHO	IC₅₀	0.002 μM Compound: DuP-697	In vitro inhibition of PGE-2 produced by arachidonic acid-stimulated CHO cells stably expressing human Prostaglandin G/H synthase 2 In vitro inhibition of PGE-2 produced by arachidonic acid-stimulated CHO cells stably expressing human Prostaglandin G/H synthase 2	[PMID: 9873621]
CHO	IC₅₀	0.0021 μM Compound: DuP-697	Inhibition of PGE-2 production by arachidonic acid-stimulated CHO cells expressing human Prostaglandin G/H synthase 2 Inhibition of PGE-2 production by arachidonic acid-stimulated CHO cells expressing human Prostaglandin G/H synthase 2	10.1016/S0960-894X(96)00501-X
CHO	IC₅₀	0.059 μM Compound: DuP-697	Inhibition of PGE-2 production in arachidonic acid-stimulated CHO cells expressing human Prostaglandin G/H synthase 1 Inhibition of PGE-2 production in arachidonic acid-stimulated CHO cells expressing human Prostaglandin G/H synthase 1	10.1016/S0960-894X(96)00501-X
CHO	IC₅₀	0.059 μM Compound: DuP-697	In vitro inhibition of the production of PGE-2 in arachidonic acid stimulated chinese hamster ovary (CHO) cells transfected with human cyclooxygenase-1 In vitro inhibition of the production of PGE-2 in arachidonic acid stimulated chinese hamster ovary (CHO) cells transfected with human cyclooxygenase-1	10.1016/S0960-894X(96)00580-X
CHO	IC₅₀	0.059 μM Compound: DuP-697	Tested in vitro for the ability to inhibit Prostaglandin G/H synthase 1 in chinese hamster ovary (CHO) cells Tested in vitro for the ability to inhibit Prostaglandin G/H synthase 1 in chinese hamster ovary (CHO) cells	10.1016/S0960-894X(96)00582-3
Sf21	IC₅₀	126.32 μM Compound: Dup-697	Inhibition of human recombinant COX2 expressed in baculovirus infected SF21 cell Inhibition of human recombinant COX2 expressed in baculovirus infected SF21 cell	[PMID: 20974503]
Sf9	IC₅₀	0.005 μM Compound: 1 (DuP-697)	Inhibitory activity against human COX-2 expressed in baculovirus infected Sf9 cells Inhibitory activity against human COX-2 expressed in baculovirus infected Sf9 cells	10.1016/0960-894X(95)00359-2
Sf9	IC₅₀	0.007 μM Compound: DuP-697	Inhibitory activity against human recombinant Prostaglandin G/H synthase 2 expressed in microsomes taken from baculovirus infected Sf9 cells Inhibitory activity against human recombinant Prostaglandin G/H synthase 2 expressed in microsomes taken from baculovirus infected Sf9 cells	10.1016/0960-894X(95)00564-A
Sf9	IC₅₀	0.6 μM Compound: 1 (DuP-697)	Inhibitory activity against human COX-1 expressed in baculovirus infected Sf9 cells Inhibitory activity against human COX-1 expressed in baculovirus infected Sf9 cells	10.1016/0960-894X(95)00359-2
Sf9	IC₅₀	0.6 μM Compound: DuP-697	Inhibitory activity against human recombinant Prostaglandin G/H synthase 1 expressed in microsomes taken from baculovirus infected Sf9 cells Inhibitory activity against human recombinant Prostaglandin G/H synthase 1 expressed in microsomes taken from baculovirus infected Sf9 cells	10.1016/0960-894X(95)00564-A
U-937	IC₅₀	0.007 μM Compound: DuP-697	Inhibition of Prostaglandin G/H synthase 1 was measured by the inhibition of PGE-2 produced by microsomes from U937 cells at subsaturating arachidonic acid conc. Inhibition of Prostaglandin G/H synthase 1 was measured by the inhibition of PGE-2 produced by microsomes from U937 cells at subsaturating arachidonic acid conc.	[PMID: 9873621]

In Vitro

DuP-697 (0-100 nM; 24 hours; HT29 cells) treatment shows antiproliferative with an IC₅₀ value of 42.8 nM^[1].
DuP-697 (25-100 nM; 72 hours; HT29 cells) treatment causes concentration dependent apoptosis in HT29 cells. The percentage of UR (apoptosis portion) area increases gradually according to the concentration of DuP-697 from 7% in control group to 52% in 100 nM DuP-697^[1].
DuP-697 in 100, 10 and 1 nM concentrations cause antiangiogenic effect. Antiangiogenic scores of DuP-697 are 1.2, 0.8 and 0.5, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	HT29 cells
Concentration:	0 nM, 12.5 nM, 25 nM, 50 nM, 100 nM
Incubation Time:	24 hours
Result:	Exhibited decreasing CI values in a concentration dependent manner. Showed statistically significant cytotoxic effect.

Apoptosis Analysis^[1]

Cell Line:	HT29 cells
Concentration:	25 nM, 50 nM, 100 nM
Incubation Time:	72 hours
Result:	Caused concentration dependent apoptosis in HT29 cells.

In Vivo

DuP-697 is a potent inhibitor of paw swelling in nonestablished and established adjuvant arthritis in rats (ED₅₀ = 0.03 and 0.18 mg/kg/day, respectively). DuP-697 has no effect on phenylquinone writhing in rats (ED₅₀ greater than 100 mg/kg), but is analgetic against inflammation-related pain in the Randall-Selitto assay (ED₅₀ = 3.5 mg/kg) and is a very potent antipyretic agent (ED₅₀ = 0.05 mg/kg). DuP-697 (5 mg/kg i.v.) does not alter renal blood flow or the renal vascular response to angiotensin II in furosemide-pretreated, volume-depleted rats^[2].
DuP-697 is a moderate inhibitor of bull seminal vesicle prostaglandin (PG) synthesis (IC₅₀ of 24 μM) and a potent inhibitor of rat brain PG synthesis (IC₅₀ of 4.5 μM) but was ineffective against rat kidney PG synthesis (IC₅₀ of 75 μM)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

411.31

Formula

C₁₇H₁₂BrFO₂S₂

CAS No.

88149-94-4

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(C2=C(C3=CC=C(F)C=C3)SC(Br)=C2)C=C1)(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (243.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : ≥ 54 mg/mL (131.29 mM)

Ethanol : ≥ 7 mg/mL (17.02 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4313 mL	12.1563 mL	24.3126 mL
5 mM	0.4863 mL	2.4313 mL	4.8625 mL
10 mM	0.2431 mL	1.2156 mL	2.4313 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (283 KB) SDS (251 KB)

COA (257 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Altun A, et al. Anticancer effect of COX-2 inhibitor DuP-697 alone and in combination with tyrosine kinase inhibitor (E7080) on colon cancer cell lines. Asian Pac J Cancer Prev. 2014;15(7):3113-21. [Content Brief]

[2]. Gans KR, et al. Anti-inflammatory and safety profile of DuP 697, a novel orally effective prostaglandin synthesis inhibitor. J Pharmacol Exp Ther. 1990 Jul;254(1):180-7. [Content Brief]

[3]. Gierse JK, et al. Expression and selective inhibition of the constitutive and inducible forms of human cyclo-oxygenase. Biochem J. 1995 Jan 15;305 ( Pt 2):479-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMF / DMSO	1 mM	2.4313 mL	12.1563 mL	24.3126 mL	60.7814 mL
	5 mM	0.4863 mL	2.4313 mL	4.8625 mL	12.1563 mL
	10 mM	0.2431 mL	1.2156 mL	2.4313 mL	6.0781 mL
	15 mM	0.1621 mL	0.8104 mL	1.6208 mL	4.0521 mL
DMF / DMSO	20 mM	0.1216 mL	0.6078 mL	1.2156 mL	3.0391 mL
	25 mM	0.0973 mL	0.4863 mL	0.9725 mL	2.4313 mL
	30 mM	0.0810 mL	0.4052 mL	0.8104 mL	2.0260 mL
	40 mM	0.0608 mL	0.3039 mL	0.6078 mL	1.5195 mL
	50 mM	0.0486 mL	0.2431 mL	0.4863 mL	1.2156 mL
	60 mM	0.0405 mL	0.2026 mL	0.4052 mL	1.0130 mL
	80 mM	0.0304 mL	0.1520 mL	0.3039 mL	0.7598 mL
	100 mM	0.0243 mL	0.1216 mL	0.2431 mL	0.6078 mL

DuP-697 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

DuP-697

Customer Review

DuP-697 Related Antibodies

1 Publications Citing Use of MCE DuP-697

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All COX Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

DuP-697 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DuP-697 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report