Isoprenaline hydrochloride
Based on 89 publication(s) in Google Scholar
Isoprenaline (Isoproterenol) hydrochloride is a non-selective, orally active β-adrenergic receptor agonist. Isoprenaline has potent peripheral vasodilator, bronchodilator, and cardiac stimulating activities. Isoprenaline can be used for the research of bradycardia and bronchial asthma.
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- Pureza: 99.86%
- No. CAS: 51-30-9
- Fòrmula: C11H18ClNO3
- Peso molecular:247.72
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Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Isoprenaline hydrochloride
More- Nature. 2025 Dec;648(8092):146-156. [Abstract]
- Science. 2020 Dec 4;370(6521):eaay2002. [Abstract]
- Circulation. 2018 Jun 5;137(23):2497-2513. [Abstract]
- Cancer Discov. 2026 Feb 9. [Abstract]
- Sci Bull. 2024 Dec 30;69(24):3908-3924. [Abstract]
- Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
- ACS Nano. 2023 Nov 14;17(21):21639-21661. [Abstract]
- Nat Commun. 2026 Jan 16;17(1):1795. [Abstract]
- Nat Commun. 2020 Sep 25;11(1):4857. [Abstract]
- Cell Death Differ. 2023 Mar;30(3):766-778. [Abstract]
- Acta Pharm Sin B. 2026 Mar 14.
- Chem Eng J. 2024 Sep 1.
- J Adv Res. 2024 Dec 28:S2090-1232(24)00624-6. [Abstract]
- J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. [Abstract]
- Redox Biol. 2024 Dec 21:79:103481. [Abstract]
- J Immunother Cancer. 2025 Jun 12;13(6):e011611. [Abstract]
- J Pharm Anal. 2024 Jul;14(7):100934. [Abstract]
- Acta Pharmacol Sin. 2025 Jun 11. [Abstract]
- Phytomedicine. 2026 Jun:155:158109. [Abstract]
- ACS Appl Mater Interfaces. 2023 Oct 18;15(41):47939-47954. [Abstract]
- ACS Appl Mater Interfaces. 2023 Apr 12;15(14):17518-17531. [Abstract]
- Biofabrication. 2022 Oct 28;15(1). [Abstract]
- J Transl Med. 2025 Jul 10;23(1):762. [Abstract]
- Emerg Microbes Infect. 2025 Mar 24:2484330. [Abstract]
- Biomed Pharmacother. 2021 Jun:138:111531. [Abstract]
- Biomed Pharmacother. 2021 Jul:139:111562. [Abstract]
- Cell Rep. 2022 Mar 8;38(10):110468. [Abstract]
- Cell Rep. 2019 Dec 3;29(10):2929-2935.e4 [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119433. [Abstract]
- Chin Med. 2024 Jun 11;19(1):82. [Abstract]
- Biochem Pharmacol. 2025 Aug 8;242(Pt 1):117218. [Abstract]
- Biochem Pharmacol. 2024 May 29:116329. [Abstract]
- Food Funct. 2018 Nov 14;9(11):5528-5535. [Abstract]
- Life Sci. 2026 Apr 15:391:124281. [Abstract]
- Life Sci. 2026 Mar 1:388:124212. [Abstract]
- Life Sci. 2025 Dec 15:383:124083. [Abstract]
- Inflammation. 2024 Apr;47(2):789-806. [Abstract]
- Nutrients. 2023 Mar 9;15(6):1340. [Abstract]
- Biomolecules. 2022 Oct 27;12(11):1574. [Abstract]
- Pharmaceuticals (Basel). 2022 Jan 6;15(1):73. [Abstract]
- Biosci Rep. 2026 May 20;46(5):BSR20250174. [Abstract]
- Eur J Pharmacol. 2024 Oct 30:177085. [Abstract]
- Int Immunopharmacol. 2023 Sep 5;124(Pt A):110876. [Abstract]
- mBio. 2022 Dec 20;13(6):e0229722. [Abstract]
- Eur J Pharmacol. 2021 Sep 15:907:174277. [Abstract]
- Eur J Pharmacol. 2020 Nov 5;886:173439. [Abstract]
- Molecules. 2024 Feb 7;29(4):760. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Dec 31;1872(3):168150. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Mar;1870(3):167018. [Abstract]
- FASEB J. 2022 Mar;36(3):e22219. [Abstract]
- iScience. 2026 Jan 7;29(2):114639. [Abstract]
- J Inflamm Res. 2023 Oct 24:16:4833-4843. [Abstract]
- iScience. 2023 Jul 22;26(9):107455. [Abstract]
- J Funct Foods. 66 (2020) 1038022.
- Sci Rep. 2024 Jul 8;14(1):15683. [Abstract]
- Mol Cell Biochem. 2026 Jan 22. [Abstract]
- Mol Med Rep. 2026 Feb;33(2):60. [Abstract]
- Food Chem Toxicol. 2023 Apr:174:113670. [Abstract]
- Toxicol Appl Pharmacol. 2025 Oct:503:117473. [Abstract]
- Cell Cycle. 2021 Jul;20(13):1253-1269. [Abstract]
- Chem Biol Drug Des. 2024 Feb 6.
- Front Oncol. 2021 Apr 22:11:665763. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2025 Mar 17. [Abstract]
- Cell Biol Int. 2021 Jan;45(1):188-197. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2018 Dec;391(12):1373-1385. [Abstract]
- Mol Immunol. 2025 Dec:188:45-61. [Abstract]
- Curr Issues Mol Biol. 2025 Sep 26;47(10):798. [Abstract]
- J Tradit Complement Med. 2025 Mar 14.
- J Tradit Complement Med. 2023 Jul 12;14(1):40-54. [Abstract]
- J Cardiovasc Transl Res. 2023 Aug;16(4):758-767. [Abstract]
- Prostate. 2020 Nov;80(15):1328-1340. [Abstract]
- Int J Exp Pathol. 2025 Mar;106(2):e12526. [Abstract]
- J Mol Cell Cardiol Plus. 2024 Dec:10:100121. [Abstract]
- Biochem Biophys Res Commun. 2022 Feb 12:592:67-73. [Abstract]
- Int J Cardiovasc Imaging. 2025 Apr 10. [Abstract]
- Acta Pharm. 2026 Mar 25;76(1):1-12. [Abstract]
- Nat Prod Commun. 2023, 18(1): 1934578X221144581.
- Biomed Res. 2023;44(3):105-115. [Abstract]
- bioRxiv. 2026 Jun 19.
- bioRxiv. 2025 July 11.
- bioRxiv. 2025 Jul 30.
- SSRN. 2025 May 8.
- Res Sq. 2024 Sep 30.
- bioRxiv. 2024 Apr 20:2024.04.15.589650. [Abstract]
- Research Square Preprint. 2023 Sep 14.
- Research Square Preprint. 2023 Sep 14.
- Research Square Print. 2023 Feb 7.
- Research Square Preprint. 2021 Jan.
- bioRxiv. 2020 Jan.
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Actividad biológica
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β adrenergic receptor |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | EC50 |
240 nM
Compound: Isoproterenol, S/R-Isoprenaline
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Agonist activity at recombinant human beta2 adrenoceptor expressed in HEK293T cells assessed as increase in IBMX-induced cAMP accumulation using [3H]-cAMP after 30 mins by liquid scintillation counting
Agonist activity at recombinant human beta2 adrenoceptor expressed in HEK293T cells assessed as increase in IBMX-induced cAMP accumulation using [3H]-cAMP after 30 mins by liquid scintillation counting
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[PMID: 22182578] |
Isoprenaline (Isoproterenol) hydrochloride (300 nM, 3 min) increases particulate cGMP- and cilostamide-inhibited, low-Km cAMP phosphodiesterase (cAMP-PDE) activity by about 100% in intact rat fat cells[1].
Isoprenaline inhibits insulin-stimulated glucose transport activity in rat adipocytes. Isoprenaline, in the absence of adenosine, promotes a time-dependent (t1/2 approximately 2 min) decrease in the accessibility of insulin-stimulated cell surface GLUT4 of > 50%, which directly correlated with the observed inhibition of transport activity[2].
Isoprenaline (5 nM and 10 μM) increases cyclic AMP levels and this effect is potentiated by cilostamide (10 mM), by rolipram, a cyclic AMP-specific PDE (PDE 4) inhibitor (10 mM) and by cyclic GMP-elevating agents (50 nM ANF or 30 nM SNP plus 100 nM DMPPO)[3].
Isoprenaline increases the transcriptional activity of Gi alpha-2 gene to 140% of the control value, whereas gene specific hybridization for Gs alpha remains unchanged[4].
Isoprenaline (20 nM) increases the amplitude of total iK and causes a negative shift of approximately 10 mV in the activation curve for iK, both in the absence and in the presence of 300 nM nisoldipine to block the L-type Ca2+ current[5].
Isoprenaline (20 nM) increases the spontaneous pacemaker rate of sino-atrial node pacemaker cells by 16% in rabbit isolated pacemaker cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Isoprenaline (Isoproterenol) hydrochloride (oral, 0.27-0. 64 μg/kg) is extensively metabolizes by a relatively small number of reactions in dogs[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Dogs[1]
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Dosage:0.27-0. 64 μg/kg
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Administration:oral
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Result:Excreted largely unchanged in urine, only one-third of the radioactivity in urine was in the form of the O-methyl metabolite.
Showed plasma radioactivity was almost entirely as conjugated isoprenaline and this metabolite accounted for more than 80% of radioactivity in urine.
. Showed heart rate returned to base-line values when high plasma concentrations.
Chemical Information
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No. CAS 51-30-9
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Appearance Solid
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Peso molecular 247.72
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Fòrmula C11H18ClNO3
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Color White to off-white
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SMILES
OC1=CC=C(C(O)CNC(C)C)C=C1O.Cl
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Synonyms
Isoproterenol hydrochloride
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (89)
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Journal Impact Factor
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Most Recent
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Nature
2025 Dec;648(8092):146-156. PMID: 41094146 -
Science
2020 Dec 4;370(6521):eaay2002. PMID: 33093214 -
Circulation
Targeting Chondroitin Sulfate Glycosaminoglycans to Treat Cardiac Fibrosis in Pathological Remodeling. [Abstract]2018 Jun 5;137(23):2497-2513. PMID: 29371215 -
Cancer Discov
Myofibroblasts Induce Neuroplasticity to Promote Pancreatic Inflammation and Cancer Progression. [Abstract]2026 Feb 9. PMID: 41661076 -
Sci Bull
Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes. [Abstract]2024 Dec 30;69(24):3908-3924. PMID: 39550273 -
Cell Mol Immunol
β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response. [Abstract]2023 Feb;20(2):175-188. PMID: 36600052
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
Isoprenaline hydrochloride (ISO; 10 μM; 6 h), markedly inhibits SeV- or HSV-1-induced transcription of the IFNB1 and CXCL10 genes in THP1 cells.
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
Isoprenaline hydrochloride (ISO; 10 μM; 0, 3, 6, 9 h) inhibits the phosphorylation of MITAS366, IRF3S386 and STAT1Y701 induced by HSV-1, HSV-2 and HCMV, as well as the phosphorylation of IRF3S386 and STAT1Y701 induced by SeV, EMCV and VSV, in THP1 cells.
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ACS Nano
Red Blood Cell-Derived Extracellular Vesicles Display Endogenous Antiviral Effects and Enhance the Efficacy of Antiviral Oligonucleotide Therapy. [Abstract]2023 Nov 14;17(21):21639-21661. PMID: 37852618 -
Nat Commun
An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis. [Abstract]2026 Jan 16;17(1):1795. PMID: 41545427 -
Nat Commun
DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. [Abstract]2020 Sep 25;11(1):4857. PMID: 32978402 -
Cell Death Differ
Blockage of PPARγ T166 phosphorylation enhances the inducibility of beige adipocytes and improves metabolic dysfunctions. [Abstract]2023 Mar;30(3):766-778. PMID: 36329235
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Mar;30(3):766-778. [Abstract]
SVF differentiated adipocytes were treated with 2 μM isoprenaline (ISO) for 12h followed by Q-PCR analysis of browning genes (n=3). Gene expression is normalized to the 36B4 endogenous control.
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Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Sep 1.
Isoprenaline administration significantly reduces [email protected] anti-inflammatory and antioxidant activities.
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Sep 1.
Isoprenaline significantly activates ERK1/2-induced DRP1 phosphorylation on serine 616 (pDRP1).
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J Adv Res
Elevated EBF2 in mouse but not pig drives the progressive brown fat lineage specification via chromatin activation. [Abstract]2024 Dec 28:S2090-1232(24)00624-6. PMID: 39736442 -
J Exp Clin Cancer Res
Mammalian Eps15 homology domain 1 potentiates angiogenesis of non-small cell lung cancer by regulating β2AR signaling. [Abstract]2019 Apr 25;38(1):174. PMID: 31023336
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. [Abstract]
EHD1 stabilizes β2AR. A549 cells are incubated in serum-free 1640 medium for 16 h and then treated with ISO (10 μM) for the indicated times in the presence of cycloheximide (CHX, 20 μg/mL). The cell lysates are analyzed by Western blot.
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. [Abstract]
A549 cells were transfected with vector or ShEHD1 and then treated with DMSO or ISO (Isoprenaline) (10 μM; 16 h), and the EHD1 and VEGFA protein levels were assessed by immunoblotting.
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Redox Biol
2024 Dec 21:79:103481. PMID: 39721495 -
J Immunother Cancer
ADRB2 inhibition suppresses cancer immune evasion by regulating tumor SOX10-PD-L1 axis and T cell function. [Abstract]2025 Jun 12;13(6):e011611. PMID: 40514069 -
J Pharm Anal
Baicalin reduces chronic stress-induced breast cancer metastasis via directly targeting β2-adrenergic receptor. [Abstract]2024 Jul;14(7):100934. PMID: 39139999 -
Acta Pharmacol Sin
YOD1 mediates isoproterenol-induced cardiac remodeling by deubiquitinating PKM2 and reducing PKM2 tetramerization in cardiomyocytes. [Abstract]2025 Jun 11. PMID: 40500343 -
Phytomedicine
Wendan tablet and its active component isocurcumenol protect against myocardial ischemia via targeting ALOX15 and inhibiting lipid peroxidation. [Abstract]2026 Jun:155:158109. PMID: 41966026 -
ACS Appl Mater Interfaces
Insights into the Mechanism of Supramolecular Self-Assembly in the Astragalus membranaceus- Angelica sinensis Codecoction. [Abstract]2023 Oct 18;15(41):47939-47954. PMID: 37791782 -
ACS Appl Mater Interfaces
Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes. [Abstract]2023 Apr 12;15(14):17518-17531. PMID: 36992621 -
Biofabrication
Heart-on-a-chip using human iPSC-derived cardiomyocytes with integrated vascular endothelial layer based on culture patch as a potential platform for drug evaluation. [Abstract]2022 Oct 28;15(1). PMID: 36195063 -
J Transl Med
Gut microbiota dysbiosis exacerbates heart failure by the LPS-TLR4/NF-κB signalling axis: mechanistic insights and therapeutic potential of TLR4 inhibition. [Abstract]2025 Jul 10;23(1):762. PMID: 40640887 -
Emerg Microbes Infect
Unique immune and other responses of human nasal epithelial cells infected with H5N1 avian influenza virus compared to seasonal human influenza A and B viruses. [Abstract]2025 Mar 24:2484330. PMID: 40126073 -
Biomed Pharmacother
Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. [Abstract]2021 Jun:138:111531. PMID: 34311530 -
Biomed Pharmacother
Periplocymarin protects against myocardial fibrosis induced by β-adrenergic activation in mice. [Abstract]2021 Jul:139:111562. PMID: 33839492 -
Cell Rep
Moderate heart rate reduction promotes cardiac regeneration through stimulation of the metabolic pattern switch. [Abstract]2022 Mar 8;38(10):110468. PMID: 35263588 -
Cell Rep
2019 Dec 3;29(10):2929-2935.e4 PMID: 31801060 -
Ecotoxicol Environ Saf
PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]2025 Nov 15:307:119433. PMID: 41273832 -
Chin Med
Xinbao Pill ameliorates heart failure via regulating the SGLT1/AMPK/PPARα axis to improve myocardial fatty acid energy metabolism. [Abstract]2024 Jun 11;19(1):82. PMID: 38862959 -
Biochem Pharmacol
2025 Aug 8;242(Pt 1):117218. PMID: 40784571 -
Biochem Pharmacol
FGF13 deficiency ameliorates calcium signaling abnormality in heart failure by regulating microtubule stability. [Abstract]2024 May 29:116329. PMID: 38821375 -
Food Funct
Correlation of drug-induced and drug-related ultra-high performance liquid chromatography-mass spectrometry serum metabolomic profiles yields discovery of effective constituents of Sini decoction against myocardial ischemia in rats. [Abstract]2018 Nov 14;9(11):5528-5535. PMID: 30209468 -
Life Sci
Oxytocin ameliorates cardiac hypertrophy by inhibiting mitochondrial dysfunction and pyroptosis via AMPK/PGC-1α /TFAM pathway. [Abstract]2026 Apr 15:391:124281. PMID: 41722768 -
Life Sci
iNKT cell activation exacerbates isoproterenol-induced cardiac injury through macrophage IFN-γ-STAT1 signaling. [Abstract]2026 Mar 1:388:124212. PMID: 41539464 -
Life Sci
Dapagliflozin attenuates post-infarction fibrosis via cardiomyocyte protection and fibroblast inhibition. [Abstract]2025 Dec 15:383:124083. PMID: 41224193 -
Inflammation
2024 Apr;47(2):789-806. PMID: 38446361 -
Nutrients
Naringenin Attenuates Isoprenaline-Induced Cardiac Hypertrophy by Suppressing Oxidative Stress through the AMPK/NOX2/MAPK Signaling Pathway. [Abstract]2023 Mar 9;15(6):1340. PMID: 36986070 -
Biomolecules
Inhibition of Wdr5 Attenuates Ang-II-Induced Fibroblast-to-Myofibroblast Transition in Cardiac Fibrosis by Regulating Mdm2/P53/P21 Pathway. [Abstract]2022 Oct 27;12(11):1574. PMID: 36358925 -
Pharmaceuticals (Basel)
2022 Jan 6;15(1):73. PMID: 35056130 -
Biosci Rep
Isoprenaline alleviates diabetic kidney disease via multi-target inhibition of the cGAS-STING pathway. [Abstract]2026 May 20;46(5):BSR20250174. PMID: 41795784 -
Eur J Pharmacol
miR-421-mediated suppression of FGF13 as a novel mechanism ameliorates cardiac hypertrophy by inhibiting endoplasmic reticulum stress. [Abstract]2024 Oct 30:177085. PMID: 39486770 -
Int Immunopharmacol
Germacrone mitigates cardiac remodeling by regulating PI3K/AKT-mediated oxidative stress, inflammation, and apoptosis. [Abstract]2023 Sep 5;124(Pt A):110876. PMID: 37683399 -
mBio
Grass Carp Reovirus Induces Formation of Lipid Droplets as Sites for Its Replication and Assembly. [Abstract]2022 Dec 20;13(6):e0229722. PMID: 36445081 -
Eur J Pharmacol
Protective effects of dioscin against isoproterenol-induced cardiac hypertrophy via adjusting PKCε/ERK-mediated oxidative stress. [Abstract]2021 Sep 15:907:174277. PMID: 34171391 -
Eur J Pharmacol
Tempol prevents isoprenaline-induced takotsubo syndrome via the reactive oxygen species/mitochondrial/anti-apoptosis /p38 MAPK pathway. [Abstract]2020 Nov 5;886:173439. PMID: 32871175 -
Molecules
Chlorogenic Acid Attenuates Isoproterenol Hydrochloride-Induced Cardiac Hypertrophy in AC16 Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway. [Abstract]2024 Feb 7;29(4):760. PMID: 38398512 -
Biochim Biophys Acta Mol Basis Dis
Osteopontin aggravates myocardial fibrosis by promoting phenotypic transition of cardiac fibroblasts via Hippo-YAP pathway. [Abstract]2025 Dec 31;1872(3):168150. PMID: 41478364 -
Biochim Biophys Acta Mol Basis Dis
OTUD1 promotes isoprenaline- and myocardial infarction-induced heart failure by targeting PDE5A in cardiomyocytes. [Abstract]2024 Mar;1870(3):167018. PMID: 38185350 -
FASEB J
RNA-binding protein YBX1 promotes brown adipogenesis and thermogenesis via PINK1/PRKN-mediated mitophagy. [Abstract]2022 Mar;36(3):e22219. PMID: 35195911 -
iScience
Thrombospondin 1 aggravates cardiac remodeling in heart failure with preserved ejection fraction by inhibiting mitophagy. [Abstract]2026 Jan 7;29(2):114639. PMID: 41623478 -
J Inflamm Res
Creation of a Rat Takotsubo Syndrome Model and Utilization of Machine Learning Algorithms for Screening Diagnostic Biomarkers. [Abstract]2023 Oct 24:16:4833-4843. PMID: 37901384 -
iScience
Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration. [Abstract]2023 Jul 22;26(9):107455. PMID: 37680481 -
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Sci Rep
Empagliflozin rescues pro-arrhythmic and Ca2+ homeostatic effects of transverse aortic constriction in intact murine hearts. [Abstract]2024 Jul 8;14(1):15683. PMID: 38977794 -
Mol Cell Biochem
Identification of disease-associated gene expression signatures for mechanistic insights and drug prediction in dilated cardiomyopathy-induced heart failure. [Abstract]2026 Jan 22. PMID: 41569495 -
Mol Med Rep
ABCC9 knockdown attenuates isoproterenol‑induced myocardial hypertrophy by inhibiting the PI3K/AKT signaling pathway. [Abstract]2026 Feb;33(2):60. PMID: 41347799 -
Food Chem Toxicol
1,3-dichloro-2-propanol caused lipid droplets accumulation by suppressing neutral lipases via BMAL1 in hepatocytes. [Abstract]2023 Apr:174:113670. PMID: 36805544
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2023 Apr:174:113670. [Abstract]
Isoprenaline hydrochloride (ISO; 10 μM; 6 h) pretreatment markedly restores the ATGL and HSL expressions decreased by 1,3-dichloro-2-propanol (1,3-DCP; 100 μM; 6 h) in HepG2 cells.
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Toxicol Appl Pharmacol
2025 Oct:503:117473. PMID: 40653114 -
Cell Cycle
Quercetin prevents isoprenaline-induced myocardial fibrosis by promoting autophagy via regulating miR-223-3p/FOXO3. [Abstract]2021 Jul;20(13):1253-1269. PMID: 34097559 -
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Front Oncol
2021 Apr 22:11:665763. PMID: 33968771 -
Naunyn Schmiedebergs Arch Pharmacol
2025 Mar 17. PMID: 40095050 -
Cell Biol Int
Bone marrow mesenchymal stem cells inhibit cardiac hypertrophy by enhancing FoxO1 transcription. [Abstract]2021 Jan;45(1):188-197. PMID: 33049085 -
Naunyn Schmiedebergs Arch Pharmacol
Protocatechuic aldehyde protects against isoproterenol-induced cardiac hypertrophy via inhibition of the JAK2/STAT3 signaling pathway. [Abstract]2018 Dec;391(12):1373-1385. PMID: 30132020
Isoprenaline hydrochloride purchased from MedChemExpress. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2018 Dec;391(12):1373-1385. [Abstract]
NRCMs are pre-incubated with PCA (50, 100, and 200 μM) for 1 h followed by 10 μM Isoproterenol (ISO) treatment. Cell surface area is measured by rhodamine-phalloidin staining. The expression of β-MHC is detected by Western blot.
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Mol Immunol
Plasma secretory protein genes in hepatocellular carcinoma and heart failure: Comorbidity and biological function exploration. [Abstract]2025 Dec:188:45-61. PMID: 41192015 -
Curr Issues Mol Biol
Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays. [Abstract]2025 Sep 26;47(10):798. PMID: 41150746 -
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J Tradit Complement Med
Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction. [Abstract]2023 Jul 12;14(1):40-54. PMID: 38223805 -
J Cardiovasc Transl Res
2023 Aug;16(4):758-767. PMID: 36715820 -
Prostate
β2 -adrenergic receptor signaling drives prostate cancer progression by targeting the Sonic hedgehog-Gli1 signaling activation. [Abstract]2020 Nov;80(15):1328-1340. PMID: 32894788 -
Int J Exp Pathol
KLF9 aggravates the cardiomyocyte hypertrophy in hypertrophic obstructive cardiomyopathy through the lncRNA UCA1/p27 axis. [Abstract]2025 Mar;106(2):e12526. PMID: 39909852 -
J Mol Cell Cardiol Plus
Dual calcium-voltage optical mapping of regional voltage and calcium signals in intact murine RyR2-R2474S hearts. [Abstract]2024 Dec:10:100121. PMID: 39697246 -
Biochem Biophys Res Commun
2022 Feb 12:592:67-73. PMID: 35032834 -
Int J Cardiovasc Imaging
Assessment of left ventricular systolic function and pathological changes using layer-specific strain in rats with myocardial hypertrophy at various disease stages. [Abstract]2025 Apr 10. PMID: 40208430 -
Acta Pharm
The effect of astragaloside IV on a model of isoproterenol-induced hypertrophic injury in H9c2 cells. [Abstract]2026 Mar 25;76(1):1-12. PMID: 41885696 -
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Biomed Res
The lncRNA ADAMTS9-AS1/miR-185-5p/KAT7 ceRNA network inhibits cardiomyocyte hypertrophy in hypertrophic obstructive cardiomyopathy. [Abstract]2023;44(3):105-115. PMID: 37258203 -
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bioRxiv
Functional cardiac consequences of β-adrenergic stress-induced injury in the mdx mouse model of Duchenne muscular dystrophy. [Abstract]2024 Apr 20:2024.04.15.589650. PMID: 38659739 -
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Solvente y solubilidad
DMSO : 80 mg/mL (322.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 50 mg/mL (201.84 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (403.68 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureza y Documentación
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Ficha de datos (279 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Degerman E, et al. Evidence that insulin and isoprenaline activate the cGMP-inhibited low-Km cAMP phosphodiesterase in rat fat cells by phosphorylation. Proc Natl Acad Sci U S A. 1990 Jan;87(2):533-7 [Content Brief]
[2]. Vannucci SJ, et al. Cell surface accessibility of GLUT4 glucose transporters in insulin-stimulated rat adipose cells. Modulation by isoprenaline and adenosine. Biochem J. 1992 Nov 15;288 (Pt 1):325-30. [Content Brief]
[3]. Delpy E, et al. Effects of cyclic GMP elevation on isoprenaline-induced increase in cyclic AMP and relaxation in rat aortic smooth muscle: role of phosphodiesterase 3. Br J Pharmacol. 1996 Oct;119(3):471-8. [Content Brief]
[4]. Muller FU, et al. Isoprenaline stimulates gene transcription of the inhibitory G protein alpha-subunit Gi alpha-2 in rat heart. Circ Res. 1993 Mar;72(3):696-700. [Content Brief]
[5]. Lei M, et al. Modulation of delayed rectifier potassium current, iK, by isoprenaline in rabbit isolated pacemaker cells. Exp Physiol. 2000 Jan;85(1):27-35. [Content Brief]
[6]. M E Conolly, et al. Metabolism of isoprenaline in dog and man. Br J Pharmacol [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.0368 mL | 20.1841 mL | 40.3682 mL | 100.9204 mL |
| 5 mM | 0.8074 mL | 4.0368 mL | 8.0736 mL | 20.1841 mL | |
| 10 mM | 0.4037 mL | 2.0184 mL | 4.0368 mL | 10.0920 mL | |
| 15 mM | 0.2691 mL | 1.3456 mL | 2.6912 mL | 6.7280 mL | |
| 20 mM | 0.2018 mL | 1.0092 mL | 2.0184 mL | 5.0460 mL | |
| 25 mM | 0.1615 mL | 0.8074 mL | 1.6147 mL | 4.0368 mL | |
| 30 mM | 0.1346 mL | 0.6728 mL | 1.3456 mL | 3.3640 mL | |
| 40 mM | 0.1009 mL | 0.5046 mL | 1.0092 mL | 2.5230 mL | |
| 50 mM | 0.0807 mL | 0.4037 mL | 0.8074 mL | 2.0184 mL | |
| 60 mM | 0.0673 mL | 0.3364 mL | 0.6728 mL | 1.6820 mL | |
| 80 mM | 0.0505 mL | 0.2523 mL | 0.5046 mL | 1.2615 mL | |
| 100 mM | 0.0404 mL | 0.2018 mL | 0.4037 mL | 1.0092 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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