Cat. No.: HY-W009156

Citric acid tripotassium hydrate

(Synonyms: Potassium citrate monohydrate)

13 Cited Publications

Customer Review

Based on 13 publication(s) in Google Scholar

Citric acid tripotassium hydrate (Potassium citrate monohydrate) is a natural preservative and food tartness enhancer. Citric acid tripotassium hydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Citric acid tripotassium hydrate cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid tripotassium hydrate is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 6100-05-6
Formula: C₆H₇K₃O₈
Molecular Weight:324.41

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Citric acid tripotassium hydrate Chemical Structure

CAS No.: 6100-05-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Citric acid (0-12.5 mM; 24 h) tripotassium hydrate shows antiproliferative activity in a dose dependent manner^[3].
Citric acid (12.5 mM; 72 h) tripotassium hydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner^[3].
Citric acid (12.5 mM; 48 h) tripotassium hydrate increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

HaCaT cells
Concentration:

0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time:

24 h
Result:

Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis^[3]

Cell Line:

HaCaT cells
Concentration:

12.5 mM
Incubation Time:

0, 12, 24, 48, 72 h
Result:

Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dose-dependent manner.

Western Blot Analysis^[3]

Cell Line:

HaCaT cells
Concentration:

12.5 mM
Incubation Time:

12, 24, 48, 72 h
Result:

Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

In Vivo

Citric acid (120, 240, and 480 mg/kg; i.p.) tripotassium hydrate significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver^[1].
Citric acid (120, 240, and 480 mg/kg; i.p.) tripotassium hydrate induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes^[1].
Citric acid (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) tripotassium hydrate causes renal toxicity in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

20 g male Kunming mice^[2]
Dosage:

120, 240, 480 mg/kg
Administration:

i.p.; weekly for 3 weeks
Result:

T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.

Chemical Information

CAS No. 6100-05-6
Appearance Solid
Molecular Weight 324.41
Formula C₆H₇K₃O₈
Color White to off-white
SMILES

O=C(O[K])CC(C(O[K])=O)(O)CC(O[K])=O.O
Synonyms

Potassium citrate monohydrate
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (13)

All

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Mol Cancer

Hsa_circ_0038737 promotes PARPi resistance in castration-resistant prostate cancer via IGF2BP3-mediated DNPH1 mRNA stabilization. [Abstract]

2025 Oct 2;24(1):238. PMID: 41039575
J Exp Clin Cancer Res

Genomic profiling of a collection of patient-derived xenografts and cell lines identified ixabepilone as an active drug against chemo-resistant osteosarcoma. [Abstract]

2025 Jul 8;44(1):195. PMID: 40624718
Cell Rep Med

Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancer. [Abstract]

2026 Feb 17;7(2):102621. PMID: 41707647
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
Food Chem

Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]

2023 Mar 30;405(Pt A):134807. PMID: 36370576
Food Res Int

2026 Feb 6.
Regen Biomater

Polyzwitterion-grafted decellularized bovine intercostal arteries as new substitutes of small-diameter arteries for vascular regeneration. [Abstract]

2024 Aug 22:11:rbae098. PMID: 39224131
Life Sci

Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest. [Abstract]

2024 Jun 15:347:122682. PMID: 38702025
BMC Complement Med Ther

Hepatotoxicity prediction for traditional Chinese medicine: a two-step in silico framework integrating network and machine learning approaches. [Abstract]

2026 Apr 2;26(1):177. PMID: 41923057
Bone

The protection of nicotinamide riboside against diabetes mellitus-induced bone loss via OXPHOS. [Abstract]

2025 Apr:193:117411. PMID: 39884488
J Sep Sci

Cell Metabolomics Reveals the Hepatotoxic Mechanism of Copper in Normal Rat Liver Cells Using Reversed-Phase and Hydrophilic Interaction Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry. [Abstract]

2025 Nov;48(11):e70312. PMID: 41172059
New J Chem

03 Aug 2022.

Solvent & Solubility

In Vitro:

H₂O : 120 mg/mL (369.90 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0825 mL

15.4126 mL

30.8252 mL

5 mM

0.6165 mL

3.0825 mL

6.1650 mL

10 mM

0.3083 mL

1.5413 mL

3.0825 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (308.25 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen X, et al. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014 Mar;66(2):275-82. [Content Brief]

[2]. Chen X, Lv Q, Liu Y, Deng W. Effects of the food additive, citric acid, on kidney cells of mice. Biotech Histochem. 2015 Jan;90(1):38-44. [Content Brief]

[3]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]

[4]. Ciriminna R, et al. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017 Mar 8;11:22. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	3.0825 mL	15.4126 mL	30.8252 mL	77.0630 mL
	5 mM	0.6165 mL	3.0825 mL	6.1650 mL	15.4126 mL
	10 mM	0.3083 mL	1.5413 mL	3.0825 mL	7.7063 mL
	15 mM	0.2055 mL	1.0275 mL	2.0550 mL	5.1375 mL
	20 mM	0.1541 mL	0.7706 mL	1.5413 mL	3.8531 mL
	25 mM	0.1233 mL	0.6165 mL	1.2330 mL	3.0825 mL
	30 mM	0.1028 mL	0.5138 mL	1.0275 mL	2.5688 mL
	40 mM	0.0771 mL	0.3853 mL	0.7706 mL	1.9266 mL
	50 mM	0.0617 mL	0.3083 mL	0.6165 mL	1.5413 mL
	60 mM	0.0514 mL	0.2569 mL	0.5138 mL	1.2844 mL
	80 mM	0.0385 mL	0.1927 mL	0.3853 mL	0.9633 mL
	100 mM	0.0308 mL	0.1541 mL	0.3083 mL	0.7706 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Citric acid tripotassium hydrate

13 Cited Publications

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Citric acid tripotassium hydrate

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Publications Citing Use of MedChemExpress (MCE) Citric acid tripotassium hydrate

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (13)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Citric acid tripotassium hydrate Related Classifications

HY-W009156 Related Classifications