IR-775 chloride
Based on 1 Customer Validation
IR-775 chloride is a cyanine dye with near-infrared absorption (Ex/Em = 775/795 nm). IR-775 chloride acts as a photosensitizer or photothermal agent, and induces apoptosis via generating ROS or exerting photothermal effects upon irradiation; its combination with 2-methoxyestradiol (HY-12033) inhibits SOD2 to enhance photodynamic therapy (PDT) efficacy, and its liposomal encapsulation enables phytotherapy-photothermal therapy (PTT) synergy. IR-775 chloride is applicable for investigating PDT/PTT and near-infrared fluorescence imaging in ovarian cancer and breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 199444-11-6
- Formula: C32H36Cl2N2
- Molecular Weight:519.55
-
Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
IR-775 (2-10 μM; 24-72 h) chloride is a potent photosensitizer in proliferative diabetic retinopathy (PDR). It reduces the viability of human ovarian adenocarcinoma cell line (SKOV-3) and human breast adenocarcinoma cell line (MDA-MB-231) in a time-dependent manner, and exhibits stronger cytotoxicity when combined with 2-methoxyestradiol plus irradiation[1].
IR-775 (6 μM; 2-24 h) chloride accumulates in non-nuclear organelles of human ovarian adenocarcinoma cell line (SKOV-3) and human breast adenocarcinoma cell line (MDA MB-231), and an increase in uptake is observed during the 2 h to 24 h incubation period[1].
IR-775 (6 μM; 24-72 h) chloride, when combined with 2-methoxyestradiol in a photodynamic reaction, induces oxidative stress and endoplasmic reticulum-mediated apoptosis in human ovarian adenocarcinoma cell line (SKOV-3) and human breast adenocarcinoma cell line (MDA MB-231), which is evidenced by altered SOD2 expression and increased caspase-12 expression after irradiation[1].
IR-775 (1 mg/mL) chloride can be efficiently encapsulated into spherical Hyptis suaveolens-IR-775-Liposome (HIL) NPs (141 nm), with an encapsulation efficiency of 69.13% and a drug loading rate of 17.29%. These nanoparticles exhibit characteristic near-infrared absorption/emission properties and degrade upon near-infrared laser irradiation[2].
The system of HIL nanoparticles loaded with IR-775 chloride exhibits high intracellular uptake efficiency in 4T1 cells. After irradiation with an 808 nm NIR laser, it induces significant reactive oxygen species (ROS) production, and causes over 85% of 4T1 cell death through a synergistic photothermal-photodynamic effect (CI = 0.6)[2].
HIL-loaded nanoparticles with IR-775 chloride induce > 85% cell death in 4T1 three-dimensional spheroids under 808 nm NIR laser irradiation, and can deeply penetrate into the tumor core[2].
HIL NPs loaded with IR-775 chloride disrupt the mitochondrial membrane potential of 4T1 cells, accumulate in lysosomes, and upregulate γ-H2AX, Cathepsin B, and p53 under 808 nm NIR laser irradiation, thereby inducing DNA damage and apoptosis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human ovarian adenocarcinoma (SKOV-3), human breast adenocarcinoma (MDA MB-231)
-
Concentration:2 μM; 4 μM; 6 μM; 8 μM; 10 μM
-
Incubation Time:24 h (pre-incubation); 24 h (post-irradiation incubation); 72 h (post-irradiation incubation)
-
Result:Reduced viability in both cell lines when treated with 2-10 μM for 24 h.
Identified 6 μM as the non-toxic dose.
Reduced MDA MB-231 viability to ~80% and SKOV-3 viability to ~88% after 24 h post-irradiation incubation when used alone in photodynamic reaction (PDR).
Reduced MDA MB-231 viability to 53% after 24 h and 33% after 72 h post-irradiation incubation, and SKOV-3 viability to 60% after 24 h and 45% after 72 h post-irradiation incubation when combined with 2-methoxyestradiol and irradiation.
Showed minimal cytotoxicity alone without irradiation.
-
Cell Line:human ovarian adenocarcinoma (SKOV-3), human breast adenocarcinoma (MDA MB-231)
-
Concentration:6 μM
-
Incubation Time:24 h (pre-incubation); 24 h (post-irradiation incubation); 72 h (post-irradiation incubation)
-
Result:Reduced SOD2 positive staining to 30% (weak intensity) at 24 h post-irradiation and 50% (weak intensity) at 72 h post-irradiation, while increasing caspase-12 positive staining to 80% (strong intensity) at both 24 h and 72 h post-irradiation in SKOV-3 cells.
Reduced SOD2 positive staining to 25% (moderate intensity) at 24 h post-irradiation, which returned to 100% (weak intensity) at 72 h post-irradiation, while increasing caspase-12 positive staining to 25% (weak-moderate intensity) at 24 h post-irradiation and 100% (strong intensity) at 72 h post-irradiation in MDA MB-231 cells.
Showed minimal caspase-12 staining and full SOD2 staining in unirradiated controls of both cell lines.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 199444-11-6
-
Appearance Solid
-
Molecular Weight 519.55
-
Formula C32H36Cl2N2
-
SMILES
CC1(C)C(/C=C/C2=C(Cl)/C(CCC2)=C/C=C3N(C)C4=C(C=CC=C4)C/3(C)C)=[N+](C)C5=C1C=CC=C5.[Cl-]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Waszkiewicz M, et al. The photodynamic reaction with IR-775 cyanine combined with 2-methoxyestradiol in ovarian (SKOV-3) and human breast adenocarcinoma (MDA MB-231) cell lines. Photodiagnosis and photodynamic therapy. 2022 Jun;38:102766. [Content Brief]
[2]. Pebam M, et al. IR-775 - Hyptis loaded bioactive nanoparticles for enhanced phyto-photothermal therapy of breast cancer cells. Photodiagnosis and photodynamic therapy. 2023 Dec;44:103872. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IR-775 chloride Related Classifications
HY-W248590 Related Classifications
Powered by Bioz
- IR-775
- 199444-11-6
- IR775
- IR 775
- Fluorescent Dye
- Photosensitizer
- Apoptosis
- Reactive Oxygen Species (ROS)
- SOD
- oxidative stress
- human breast adenocarcinoma (MDA MB-231) cell lines
- human breast adenocarcinoma
- apoptosis
- ovarian carcinoma
- triple-negative breast cancer
- 4T1 cells
- 4T1 3D spheroids
- hyperthermia
- human ovarian adenocarcinoma (SKOV-3) cell lines
- Inhibitor
- inhibitor
- inhibit