USP28-IN-4
Based on 1 Customer Validation
USP28-IN-4 is a USP28 inhibitor (IC50=0.04 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-4 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-4 also decreases the ankyrase-1/2 level in vitro. USP28-IN-4 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.86%
- CAS 番号: 2931509-15-6
- 分子式: C22H18Cl2N2O3S
- 分子量:461.36
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
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USP28 0.04 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
38.11 μM
Compound: 9p
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Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 3 days by SRB assay
Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 3 days by SRB assay
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[PMID: 37075624] |
| LS174T | IC50 |
24.3 μM
Compound: 9p
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Antiproliferative activity against human LS174T cells assessed as cell growth inhibition incubated for 3 days by SRB assay
Antiproliferative activity against human LS174T cells assessed as cell growth inhibition incubated for 3 days by SRB assay
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[PMID: 37075624] |
USP28-IN-4 (compound 9p) (12.5 μM, 15 μM; 3 d) inhibits colony formation of human colorectal cancer cells HCT116 (15 μM) and Ls174T (12.5 μM)[1].
USP28-IN-4 (20-80 μM; 24 h) down-regulates the level of c-Myc by enhancing its degradation via ubiquitin-proteasome system (UPS)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human colorectal cancer cells HCT116 and Ls174T
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Concentration:20 μM, 30 μM, 50 μM, and 60 μM, for Ls174T; 30 μM, 50 μM, 60 μM and 80 μM for HCT116
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Incubation Time:24 h
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Result:Dose-dependently down-regulated the cellular level of c-Myc.
化学情報
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CAS 番号 2931509-15-6
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性状 Solid
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分子量 461.36
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分子式 C22H18Cl2N2O3S
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Color White to light brown
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SMILES
CS(=O)(C1=CC=C(C(Cl)=C1)C(NC2=CC=C(C(C3=CC4=C(CNC4)C=C3)=C2)Cl)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 10 mg/mL (21.68 mM; ultrasonic and warming and adjust pH to 2 with 1 M HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1675 mL | 10.8375 mL | 21.6750 mL | 54.1876 mL |
| 5 mM | 0.4335 mL | 2.1675 mL | 4.3350 mL | 10.8375 mL | |
| 10 mM | 0.2168 mL | 1.0838 mL | 2.1675 mL | 5.4188 mL | |
| 15 mM | 0.1445 mL | 0.7225 mL | 1.4450 mL | 3.6125 mL | |
| 20 mM | 0.1084 mL | 0.5419 mL | 1.0838 mL | 2.7094 mL |
USP28-IN-4 関連分類
HY-149230 関連分類
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