Cat. No.: HY-100453

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HO-3867

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

HO-3867 is a selective and potent STAT3 inhibitor and shows good antitumor activity.

For research use only. We do not sell to patients.

Purity: 99.67%
CAS No.: 1172133-28-6
Formula: C₂₈H₃₀F₂N₂O₂
Molecular Weight:464.55

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

HO-3867 Chemical Structure

CAS No.: 1172133-28-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

HO-3867 is a selective and potent STAT3 inhibitor and shows good antitumor activity.

IC₅₀ & Target^[1]

STAT3

All STAT Isoforms :

In Vitro

HO-3867 exhibit minimal toxicity toward noncancerous cells and tissues but induce apoptosis in ovarian cancer cells. HO-3867 inhibit cell migration/invasion and survival by inhibiting STAT3 phosphorylation^[1].
BRCA-mutated ovarian cancer cells treated with HO-3867 exhibited a significant degree of apoptosis with elevated levels of cleaved caspase-3, caspase-7 and PARP^[2].
HO-3867 shows good antitumor activity at the concentration of 2 μmol/L in PANC-1 and BXPC-3 cells. Importantly, it is also found that HO-3867 treatment significantly induced reactive oxygen species (ROS) production in human pancreatic cancer cell lines, inducing PANC-1 and BXPC-3 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1172133-28-6
Appearance Solid
Molecular Weight 464.55
Formula C₂₈H₃₀F₂N₂O₂
Color Light yellow to yellow
SMILES

O=C1/C(CN(CC2=CC(C)(C)N(O)C2(C)C)C/C1=C\C3=CC=C(F)C=C3)=C/C4=CC=C(F)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All Cell Migration/Invasion AssayRT-PCRWBCell Proliferation/Viability Assay

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Journal Impact Factor

Cell Death Dis

Colorectal cancer-associated fibroblasts promote metastasis by up-regulating LRG1 through stromal IL-6/STAT3 signaling. [Abstract]

2021 Dec 20;13(1):16. PMID: 34930899

HO-3867 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Dec 20;13(1):16. [Abstract]

DLD1 and HCT-116 were cultured with control medium, CM from CAFs or treated with HO-3867 (3 μM) (STAT3 inhibitor) or pacritinib (JAK2 inhibitor), when cultured with CM from CAFs as indicated. Expression of LRG1 was subsequently analyzed by qRT-PCR.

HO-3867 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Dec 20;13(1):16. [Abstract]

DLD1 and HCT-116 were cultured with control medium, CM from CAFs or treated with HO-3867 (3 μM) (STAT3 inhibitor) or pacritinib (JAK2 inhibitor), when cultured with CM from CAFs as indicated. Expression of LRG1 was subsequently analyzed by western blot.
JCI Insight

Ruxolitinib inhibits cyclosporine-induced proliferation of cutaneous squamous cell carcinoma. [Abstract]

2018 Sep 6;3(17):e120750. PMID: 30185657

HO-3867 purchased from MedChemExpress. Usage Cited in: JCI Insight. 2018 Sep 6;3(17):e120750. [Abstract]

Proliferation assay of A431 cells exposed to ruxolitinib (10 μM); STAT1α-specific inhibitor, S14-95; and STAT3-specific inhibitor, HO-3867 (2.5 μM); alone and in combination, with and without 25 ng/ml CSA.
Biochem Pharmacol

Isoliquiritigenin inhibits the activation of the ANXA2/STAT3 pathway by down-regulating TAGLN2, thereby alleviating alcoholic fatty liver. [Abstract]

2025 Sep 6;242(Pt 2):117308. PMID: 40921221
Int J Mol Sci

Hydrogen Sulfide Prevents Elastin Loss and Attenuates Calcification Induced by High Glucose in Smooth Muscle Cells through Suppression of Stat3/Cathepsin S Signaling Pathway. [Abstract]

2019 Aug 27;20(17):4202. PMID: 31461977
J Biol Chem

STAT3 activation of SCAP-SREBP-1 signaling upregulates fatty acid synthesis to promote tumor growth. [Abstract]

2024 Jun;300(6):107351. PMID: 38718868
Invest New Drugs

Synergistic effects of the curcumin analog HO-3867 and olaparib in transforming fallopian tube epithelial cells. [Abstract]

2025 Aug 4. PMID: 40760233
J Cancer

Circulating Neutrophils Predict Poor Survival for HCC and Promote HCC Progression Through p53 and STAT3 Signaling Pathway. [Abstract]

2020 Apr 6;11(13):3736-3744. PMID: 32328178

HO-3867 purchased from MedChemExpress. Usage Cited in: J Cancer. 2020 Apr 6;11(13):3736-3744. [Abstract]

Cell migration rates of SMMC-7721 increased after co-cultured with circulating neutrophils, but decreased when treated with inhibitors of p53(Pifithrin-β) and STAT3(HO-3867) (15 μM).

HO-3867 purchased from MedChemExpress. Usage Cited in: J Cancer. 2020 Apr 6;11(13):3736-3744. [Abstract]

Number of invasion cells of MHCC-97H and SMMC-7721 increased after co-cultured with circulating neutrophils, but decreased when treated with inhibitors of p53(Pifithrin-β) and STAT3(HO-3867) (15 μM).
Research Square Preprint

2023 Jul 31.

Solvent & Solubility

In Vitro:

DMSO : ≥ 32 mg/mL (68.88 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1526 mL

10.7631 mL

21.5262 mL

5 mM

0.4305 mL

2.1526 mL

4.3052 mL

10 mM

0.2153 mL

1.0763 mL

2.1526 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (5.38 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Rath KS et al. HO-3867, a safe STAT3 inhibitor, is selectively cytotoxic to ovarian cancer. Cancer Res. 2014 Apr 15;74(8):2316-27. [Content Brief]

[2]. Hu Y, et al. A novel STAT3 inhibitor HO-3867 induces cell apoptosis by reactive oxygen species-dependent endoplasmic reticulum stress in human pancreatic cancer cells. Anticancer Drugs. 2017 Apr;28(4):392-400. [Content Brief]

[3]. Tierney BJ et al. HO-3867, a STAT3 inhibitor induces apoptosis by inactivation of STAT3 activity in BRCA1-mutated ovarian cancer cells. Cancer Biol Ther. 2012 Jul;13(9):766-75 [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1526 mL	10.7631 mL	21.5262 mL	53.8155 mL
	5 mM	0.4305 mL	2.1526 mL	4.3052 mL	10.7631 mL
	10 mM	0.2153 mL	1.0763 mL	2.1526 mL	5.3816 mL
	15 mM	0.1435 mL	0.7175 mL	1.4351 mL	3.5877 mL
	20 mM	0.1076 mL	0.5382 mL	1.0763 mL	2.6908 mL
	25 mM	0.0861 mL	0.4305 mL	0.8610 mL	2.1526 mL
	30 mM	0.0718 mL	0.3588 mL	0.7175 mL	1.7939 mL
	40 mM	0.0538 mL	0.2691 mL	0.5382 mL	1.3454 mL
	50 mM	0.0431 mL	0.2153 mL	0.4305 mL	1.0763 mL
	60 mM	0.0359 mL	0.1794 mL	0.3588 mL	0.8969 mL

Keywords

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HO-3867

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Small Molecule Immuno-Oncology Compound Library

Peptidomimetic Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) HO-3867

All STAT Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

HO-3867 Related Classifications

HY-100453 Related Classifications