Cat. No.: HY-N1353

Rhamnocitrin

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Rhamnocitrin is an anti-inflammatory and antioxidant agent that targets STIM-1, NFATc3 and MAPK pathways and can scavenge DPPH (IC₅₀=28.38 mM). Rhamnocitrin selectively inhibits oxidative stress and inflammatory responses in vascular endothelial cells and neurons. Rhamnocitrin up-regulates miR-185 to inhibit STIM-1-mediated store-operated calcium entry (SOCE), thereby blocking NFATc3 nuclear translocation and downstream inflammatory factor expression, while inducing heme oxygenase HO-1 expression and regulating the ERK/p38 MAPK pathway, inhibiting antioxidant and pro-inflammatory cytokines (such as IL-6, IL-8) and adhesion molecules (such as ICAM-1, VCAM-1). Rhamnocitrin can be used in the study of endothelial-related inflammatory diseases (such as sepsis, acute lung injury, atherosclerosis) and neuroprotection (such as oxidative damage of PC12 cells).

For research use only. We do not sell to patients.

Purity: 99.29%
CAS No.: 569-92-6
Formula: C₁₆H₁₂O₆
Molecular Weight:300.26

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Rhamnocitrin Chemical Structure

CAS No.: 569-92-6

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(In Japan)

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All p38 MAPK Isoforms :

In Vitro

Rhamnocitrin (40-80 μM; 48 h) significantly reduces apoptosis in serum deprivation-induced rat adrenal pheochromocytoma PC12 cells, and has no significant effect on STIM-1/Orai-1-mediated SOCE and downstream NFATc3 signaling^[2].
Rhamnocitrin (0.1-10 μM; 24 h) inhibits the expression of IL-6, IL-8, MCP-1, ICAM-1, VCAM-1 and PAI-1 in a concentration-dependent manner in LPS-induced human umbilical vein endothelial cells (HUVECs) experiments, and inhibits STIM-1-mediated store-operated calcium entry (SOCE)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

Human umbilical vein endothelial cells (HUVECs)
Concentration:

0.1 μM, 1 μM, 10 μM
Incubation Time:

24 h pretreatment + 6 h LPS stimulation
Result:

Dose-dependently reduced protein levels of ICAM-1, VCAM-1, and PAI-1 induced by LPS.
Significantly suppressed mRNA expressions of IL-6, IL-8, and MCP-1 at 1 μM and 10 μM.
Attenuated nuclear translocation of NFATc3, a downstream target of SOCE in a concentration-dependent manner.
Cell Viability**: No significant cytotoxicity was observed at concentrations ≤10 μM (CCK-8 assay).

Cell Viability Assay^[2]

Cell Line:

Rat pheochromocytoma PC12 cells
Concentration:

40 μM, 60 μM, 80 μM
Incubation Time:

48 h
Result:

Cell Viability: Dose-dependent increased in cell viability, with 60 μM and 80 μM groups significantly higher than the vehicle control.
Cell Apoptosis: Resulted a reduction in sub-G0/G1 phase cells (apoptotic cells) at 60 μM and 80 μM, indicating decreased apoptosis.
WB: Persistently attenuated of p38 MAPK, while increased ERK1/2 activation transiently at 60 μM.

In Vivo

After oral administration of 10 mL/kg A. complanatus extract (equivalent to 17.6 mg/kg Rhamnocitrin) to rats, collects blood (approximately 0.3 mL) at 0, 0.083, 0.167, 0.25, 0.333, 0.5, 0.75, 1, 2, 4, 6, 9, and 12 hours after administration from the ocular vein for analysis, under ether anesthesia. The results shows that the plasma concentration of Rhamnocitrin reached its maximum at 0.65 hours, with an average C_max of 29.57 ng/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 569-92-6
Appearance Solid
Molecular Weight 300.26
Formula C₁₆H₁₂O₆
Color White to yellow
SMILES

O=C1C(O)=C(C2=CC=C(O)C=C2)OC3=CC(OC)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

All Cell Proliferation/Viability AssayIFFlow CytometryWB

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Journal Impact Factor

J Ethnopharmacol

Integrating bioinformatic analysis, network pharmacology, molecular docking and experimental validation to explore the mechanism of Tian Long Cha against influenza virus. [Abstract]

2025 May 12:119964. PMID: 40368255
Sci Rep

Potential antiviral activity of rhamnocitrin against influenza virus H3N2 by inhibiting cGAS/STING pathway in vitro. [Abstract]

2024 Nov 16;14(1):28287. PMID: 39550441

Rhamnocitrin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 16;14(1):28287. [Abstract]

MDCK and A549 cells were incubated with different concentrations of Rhamnocitrin (RH) (0-100 µM). MDCK and A549 cell viability after 48 and 24 h of treatment, respectively, were measured via CCK-8 assay.

Rhamnocitrin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 16;14(1):28287. [Abstract]

Effects of Rhamnocitrin (RH) (25, 50, 100 μM) on the apoptosis of H3N2-infected MDCK cells.

Rhamnocitrin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 16;14(1):28287. [Abstract]

The effect of Rhamnocitrin (RH) (25, 50, 100 μM) on apoptotic A549 cells infected with H3N2 virus for 24 h was detected by flow cytometry analysis

Rhamnocitrin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 16;14(1):28287. [Abstract]

Protein expressions of TBK1, phosphp-TBK1 were detected via western blot analysis treated with Rhamnocitrin (RH) (25, 50, 100 μM).

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (333.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3304 mL

16.6522 mL

33.3045 mL

5 mM

0.6661 mL

3.3304 mL

6.6609 mL

10 mM

0.3330 mL

1.6652 mL

3.3304 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li Y, et al. Simultaneous Determination of Formononetin, Calycosin and Rhamnocitrin from Astragalus Complanatus by UHPLC-MS-MS in Rat Plasma: Application to a Pharmacokinetic Study. J Chromatogr Sci. 2016 Jun 19. [Content Brief]

[2]. Hong JT, et al. Regulation of heme oxygenase-1 expression and MAPK pathways in response to kaempferol and rhamnocitrin in PC12 cells. Toxicol Appl Pharmacol. 2009 May 15;237(1):59-68. [Content Brief]

[3]. Lin T, et al. Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3. Phytomedicine. 2020 Dec;79:153350. [Content Brief]

[4]. GU, Qiuli, et al. A preliminary study on anti-inflammatory effects of rhamnocitrin from Oxytropis falcata Bunge. Chinese Journal of Information on Traditional Chinese Medicine (2014): 48-50.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3304 mL	16.6522 mL	33.3045 mL	83.2612 mL
	5 mM	0.6661 mL	3.3304 mL	6.6609 mL	16.6522 mL
	10 mM	0.3330 mL	1.6652 mL	3.3304 mL	8.3261 mL
	15 mM	0.2220 mL	1.1101 mL	2.2203 mL	5.5507 mL
	20 mM	0.1665 mL	0.8326 mL	1.6652 mL	4.1631 mL
	25 mM	0.1332 mL	0.6661 mL	1.3322 mL	3.3304 mL
	30 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7754 mL
	40 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0815 mL
	50 mM	0.0666 mL	0.3330 mL	0.6661 mL	1.6652 mL
	60 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL
	80 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
	100 mM	0.0333 mL	0.1665 mL	0.3330 mL	0.8326 mL

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Rhamnocitrin

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Publications Citing Use of MedChemExpress (MCE) Rhamnocitrin

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Rhamnocitrin Related Classifications

Cat. No.: HY-N1353