2. JAK/STAT Signaling Stem Cell/Wnt
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  4. LC28

LC28 is a STAT3 inhibitor with a human STAT3 Ki of 3.92 μM.LC28 targets the DNA-binding domain of STAT3, inhibits STAT3-DNA binding, and suppresses STAT3 signaling. LC28 induces apoptosis and suppresses survival of cisplatin-resistant ovarian cancer cells.LC28 can be used for the research of cisplatin-resistant ovarian cancer.

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LC28

LC28 Chemical Structure

CAS No. : 723746-47-2

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LC28 Related Antibodies

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Description

LC28 is a STAT3 inhibitor with a human STAT3 Ki of 3.92 μM.LC28 targets the DNA-binding domain of STAT3, inhibits STAT3-DNA binding, and suppresses STAT3 signaling. LC28 induces apoptosis and suppresses survival of cisplatin-resistant ovarian cancer cells.LC28 can be used for the research of cisplatin-resistant ovarian cancer[1].

Cellular Effect
Cell Line Type Value Description References
NCI-H1299 IC50
8.1 μM
Compound: LC28
Cytotoxicity against human H1299 cells assessed as inhibition of cell survival after 72 hrs by SRB method
Cytotoxicity against human H1299 cells assessed as inhibition of cell survival after 72 hrs by SRB method
[PMID: 30145375]
SK-OV-3 IC50
3.7 μM
Compound: LC28
Cytotoxicity against human SKOV3 cells assessed as inhibition of cell survival after 72 hrs by SRB method
Cytotoxicity against human SKOV3 cells assessed as inhibition of cell survival after 72 hrs by SRB method
[PMID: 30145375]
In Vitro

LC28 (0.001-1000 μM; 10 min) inhibits STAT3-DNA binding in H1299 cell lysates overexpressing STAT3c with a Ki of 3.92 μM[1].
LC28 (0.01-1000 μM; 72 h) suppresses survival of H1299 non-small cell lung cancer cells with an IC50 of 8.1 μM after 72 h of treatment[1].
LC28 (0.01-1000 μM; 72 h) suppresses survival of SKOV3 ovarian cancer cells with an IC50 of 3.7 μM and cisplatin-resistant SKOV3/DDP ovarian cancer cells with an IC50 of 14.7 μM after 72 h of treatment[1].
LC28 (15-30 μM; 12-48 h) dose- and time-dependently inhibits constitutive and IL-6-induced STAT3 phosphorylation at Tyr705, and reduces cyclin D1 and survivin expression in cisplatin-resistant SKOV3/DDP ovarian cancer cells[1].
LC28 (15-30 μM; 48 h) induces apoptosis in cisplatin-resistant SKOV3/DDP ovarian cancer cells, with 15 μM and 30 μM treatments for 48 h increasing apoptotic cell percentages to 16% and 22%, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LC28 Related Antibodies

Cell Viability Assay[1]

Cell Line: human non-small cell lung cancer H1299 cells
Concentration: 0.01-1000 μM; 5 μM
Incubation Time: 72 h
Result: Suppressed H1299 cell survival with an IC50 of 8.1 μM.
Reduced H1299 cell survival to approximately 20% relative to vehicle control at 5 μM.

Cell Viability Assay[1]

Cell Line: human ovarian cancer SKOV3 cells, cisplatin-resistant human ovarian cancer SKOV3/DDP cells
Concentration: 0.01-1000 μM
Incubation Time: 72 h
Result: Suppressed survival of SKOV3 cells with an IC50 of 3.7 μM.
Suppressed survival of cisplatin-resistant SKOV3/DDP cells with an IC50 of 14.7 μM.

Western Blot Analysis[1]

Cell Line: cisplatin-resistant human ovarian cancer SKOV3/DDP cells
Concentration: 15 μM, 30 μM (48 h); 15 μM (12 h, 24 h, 48 h); 15 μM (48 h pretreatment prior to IL-6 stimulation)
Incubation Time: 12 h, 24 h, 48 h; 48 h (pretreatment prior to IL-6 stimulation)
Result: Reduced phosphorylation of STAT3 at Tyr705 (with minimal effect on total STAT3 protein levels) in a dose- and time-dependent manner.
Decreased IL-6-induced STAT3 phosphorylation.
Reduced protein and mRNA levels of the STAT3 downstream target genes cyclin D1 and survivin in a dose- and time-dependent manner.

Apoptosis Analysis[1]

Cell Line: cisplatin-resistant human ovarian cancer SKOV3/DDP cells
Concentration: 15 μM, 30 μM
Incubation Time: 48 h
Result: Increased the percentage of early and late apoptotic SKOV3/DDP cells to 16% relative to untreated cells at 15 μM for 48 h.
Increased the percentage of early and late apoptotic SKOV3/DDP cells to 22% relative to untreated cells at 30 μM for 48 h.
Molecular Weight

378.65

Formula

C17H13BrClNO2
CAS No.
SMILES

O=C(C=CC1=CC=C(Cl)C=C1)C2=CC(Br)=CC=C(NC)C2=O
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Room temperature in continental US; may vary elsewhere.
Storage

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Purity & Documentation
References
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LC28 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LC28723746-47-2LC 28LC-28STATH1299 non-small cell lung cancer cellsSTAT3 signalingcisplatin-resistant ovarian cancer cellsSTAT3apoptosisSKOV3 ovarian cancer cellsH1299 cell lysatesDNA-binding domainSKOV3/DDP ovarian cancer cellsIL-6Inhibitorinhibitorinhibit

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