Mitochondrial complex I-IN-1 

Mitochondrial complex I-IN-1 is a Mitochondrial complex I inhibitor. Mitochondrial complex I-IN-1 activates the AMPK signaling pathway, inhibits the downstream mTOR/S6 axis, induces Apoptosis and triggers Autophagy. Mitochondrial complex I-IN-1 can be used for the research of non-small cell lung cancer^[1].

Mitochondrial complex I-IN-1 (Compound IV-16) (0.0001-10 μM; 72 h) potently inhibits the proliferation of non-small cell lung cancer A549 cells with an IC₅₀ of 0.43 μM, while exhibits low cytotoxicity against non-small cell lung cancer H1299 cells and renal proximal tubular epithelial HK2 cells^[1].
Mitochondrial complex I-IN-1 (3 μM; 2-72 h) potently inhibits the migration of A549 cells in a time-dependent manner^[1].
Mitochondrial complex I-IN-1 (3 μM; 24 h) effectively induces apoptosis in A549 cells^[1].
Mitochondrial complex I-IN-1 (3 μM; 8 h) strongly induces autophagy in A549 cells, and its response is more significant than that of the reference compound DBI-2^[1].
Mitochondrial complex I-IN-1 (1-3 μM) activates the AMPK signaling pathway, inhibits the downstream mTOR/S6 axis, and enhances autophagy in A549 cells in a dose-dependent manner; at a concentration of 3 μM, it exhibits stronger potency than the reference compound DBI-2 (HY-162516)^[1].
Mitochondrial complex I-IN-1 specifically inhibits mitochondrial complex I in A549 cells, and this is supported by evidence that succinate supplementation restores the suppressed OCR^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

514.61

C₃₁H₃₄N₂O₅

O=C1C(C2=CC=C(OC3=CC=CC=C3)C=C2)=COC4=C1C=CC(OCCCCN5CCN(CCO)CC5)=C4

Room temperature in continental US; may vary elsewhere.

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• [1]. Liu J, et al. Design, synthesis, and mechanistic study of novel isoflavone derivatives as mitochondrial complex I inhibitors for non-small cell lung cancer treatment. Bioorganic chemistry. 2026 Sep 05;179:110036.  [Content Brief]