Cat. No.: HY-B0199A

Mycophenolate mofetil hydrochloride

(Synonyms: RS 61443 hydrochloride; TM-MMF hydrochloride)

13 Cited Publications

Customer Review

Based on 13 publication(s) in Google Scholar

Mycophenolate mofetil (RS 61443; TM-MMF) hydrochloride is an orally active antimetabolic immunosuppressant with antiproliferative and anti-inflammatory properties. Mycophenolate mofetil hydrochloride significantly inhibits the production of B, T lymphocytes and the proliferation of smooth muscle cells by selectively blocking the de novo purine synthesis pathway. Mycophenolate mofetil hydrochloride effectively reduces adventitial inflammation, medial necrosis and intimal thickening in rat aortic allografts, and decreases the number of lymphocytes expressing the IL-2 receptor, thereby delaying xenograft rejection. Mycophenolate mofetil hydrochloride shows certain toxicity in a hamster-to-rat limb xenotransplantation model when used in combination with FK506 (HY-13756). Mycophenolate mofetil hydrochloride is widely used in studies related to allograft arteriosclerosis (chronic rejection).

For research use only. We do not sell to patients.

Purity: 99.17%
CAS No.: 116680-01-4
Formula: C₂₃H₃₂ClNO₇
Molecular Weight:469.96

Storage:

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Mycophenolate mofetil hydrochloride Chemical Structure

CAS No.: 116680-01-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IL-2

All Interleukin Related Isoforms :

In Vitro

Mycophenolate mofetil hydrochloride (0-10 μg/mL; 1-3 d) significantly inhibits the in vitro proliferation of primary DA rat aortic smooth muscle cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

DA rat aortic smooth muscle cells
Concentration:

0-10 μg/mL
Incubation Time:

1-3 days
Result:

Significantly inhibited the in vitro proliferation of primary DA rat aortic smooth muscle cells.
This effect reversed after terminating incubation by medium change.

In Vivo

Mycophenolate mofetil hydrochloride (20 mg/kg/day; oral administration; once daily; from the day of transplantation to the day of sacrifice) significantly inhibits the histopathological manifestations of allograft arteriosclerosis in rat aortic allografts for up to 6 months by alleviating adventitial inflammation, medial necrosis, intimal thickening, and the proliferation of inflammatory cells and SMC^[1].
Mycophenolate mofetil hydrochloride (40 mg/kg/day; intraperitoneal injection; daily; 14 days post-injury) reduces intimal thickening and cell density in rats after carotid artery endothelial denudation, but its effect is not statistically significant^[1].
Mycophenolate mofetil hydrochloride (30 mg/kg/day; intragastric administration; once daily for 14 consecutive days) significantly delays the rejection response of hamster-to-rat limb xenotransplantation, with an average rejection time of 10.0 days^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Lewis rats (recipient); Golden Syrian hamsters (donor)^[2]
Dosage:

30 mg/kg/day
Administration:

i.g.; daily; 14 days (starting postoperative day 1)
Result:

Increased mean rejection time to 10.0 days.
Achieved a 60% survival rate.
Showed histologic grading of skin biopsies with 1 sample no rejection (R0), 3 samples lymphocytic infiltration (R1), 1 sample lymphocytic and neutrophilic infiltration (R2).
Showed histologic grading of muscle biopsies with 1 sample lymphocytic and neutrophilic infiltration (R2), 6 samples mixed cell infiltration (R3).

Chemical Information

CAS No. 116680-01-4
Appearance Solid
Molecular Weight 469.96
Formula C₂₃H₃₂ClNO₇
Color White to off-white
SMILES

O=C(OCCN1CCOCC1)CC/C(C)=C/CC2=C(O)C3=C(COC3=O)C(C)=C2OC.Cl
Synonyms

RS 61443 hydrochloride; TM-MMF hydrochloride
Structure Classification
- Phenols
- Monophenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (13)

All Flow CytometryCell Imaging/StainingCell Proliferation/Viability AssayBio/Physico-chemical AssayIn Vivo Efficacy Study

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Journal Impact Factor

J Control Release

Target-oriented delivery of self-assembled immunosuppressant cocktails prolongs allogeneic orthotopic liver transplant survival. [Abstract]

2020 Dec 10:328:237-250. PMID: 32866593
Cell Death Dis

HNF4α-HKDC1 axis orchestrates a metabolic rewiring to promote migration and metastasis in advanced gastric cancer. [Abstract]

2026 Mar 23;17(1):347. PMID: 41866549
J Transl Med

Wnt/β-catenin signalling activates IMPDH2-mediated purine metabolism to facilitate oxaliplatin resistance by inhibiting caspase-dependent apoptosis in colorectal cancer. [Abstract]

2024 Feb 3;22(1):133. PMID: 38310229
Hepatobiliary Surg Nutr

Influence of immunosuppressive drugs on natural killer cells in therapeutic drug exposure in liver transplantation. [Abstract]

2023 Dec 1;12(6):835-853. PMID: 38115918

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: Hepatobiliary Surg Nutr. 2023 Dec 1;12(6):835-853. [Abstract]

To investigate the effects of immunosuppressants on PBMCs, peripheral blood mononuclear cells from healthy volunteers were exposed to Mycophenolate Mofetil (15 µg/mL, 3 days).

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: Hepatobiliary Surg Nutr. 2023 Dec 1;12(6):835-853. [Abstract]

The cytolytic activity of NK cells on K562 and HL60 cells. NK cells sorted by MACS were exposed to TAC, CSA, Mycophenolate Mofetil (15 µg/mL, 3 days), EVE, SIR, and CS and cultured for 3 d as effector cells.
Cell Rep

HSPA6 maintains IMPDH2 phosphorylation to regulate GTP synthesis for driving radioresistance of glioblastoma stem cells. [Abstract]

2026 Jun 2;45(6):117490. PMID: 42234559
J Ethnopharmacol

Immunosuppressive effect of Columbianadin on maturation, migration, allogenic T cell stimulation and phagocytosis capacity of TNF-α induced dendritic cells. [Abstract]

2022 Mar 1:285:114918. PMID: 34919989
Life Sci

A protective role of ECSIT in chemotherapy-induced intestinal mucositis by maintaining Lgr5+ intestinal stem cells and gut homeostasis. [Abstract]

2026 Mar 15:389:124236. PMID: 41611204
Biol Proced Online

Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening. [Abstract]

2025 Jun 16;27(1):21. PMID: 40524168
J Cell Mol Med

Nanoparticle formulation of mycophenolate mofetil achieves enhanced efficacy against hepatocellular carcinoma by targeting tumour-associated fibroblast. [Abstract]

2021 Apr;25(7):3511-3523. PMID: 33713546

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]

The morphology of Mycophenolate Mofetil-LA@DSPE-PEG and Mycophenolate Mofetil-LA@PEG-PLA characterized by transmission electron microscopy (TEM).

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]

The diameters and zeta potentials of Mycophenolate Mofetil-LA@DSPE-PEG and Mycophenolate Mofetil-LA@PEG-PLA characterized by Dynamic light scattering (DLS).

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]

Cell viabilities of Huh7 cells, SUN‐449 cells, LM3 cells and Hep1‐6 cells treated with free Mycophenolate Mofetil, Mycophenolate Mofetil‐LA@DSPE‐PEG and Mycophenolate Mofetil‐LA@PEG‐PLA for 48 h.

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]

Cell cycles determined by flow cytometry. Cells were treated with free Mycophenolate Mofetil (2 µg/mL) or Mycophenolate Mofetil-LA NPs (at 2 µg/mL Mycophenolate Mofetil-equivalent dose) for 48 h.

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]

Colony Formation of Hep1-6 cells, SUN-449 cells and Huh7 cells. Hep1-6 cells, SUN-449 cells and Huh7 cells were treated with drugs at 0.5, 2 and 4 µg/mL (Mycophenolate Mofetil-equivalent concentration), respectively, for 96 h.

Mycophenolate mofetil hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]

Images of Hep1‐6 tumours after treatment with drugs at 20 mg/kg (Mycophenolate Mofetil‐equivalent concentration). Mice were orally administrated with free MMF (20 mg/kg) or intravenously injected with MMF‐LA NPs (at 20 mg/kg MMF‐equivalent dose) every other day for four times.
PLoS Negl Trop Dis

Identification of anti-flaviviral drugs with mosquitocidal and anti-Zika virus activity in Aedes aegypti. [Abstract]

2019 Aug 20;13(8):e0007681. PMID: 31430351
Exp Cell Res

Network-based analysis with primary cells reveals drug response landscape of acute myeloid leukemia. [Abstract]

2020 Aug 1;393(1):112054. PMID: 32376287
bioRxiv

2025 Sep 21.
Biomed Pharmacother

Antiviral effects of selected IMPDH and DHODH inhibitors against foot and mouth disease virus. [Abstract]

2019 Oct:118:109305. PMID: 31545264

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (425.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1278 mL

10.6392 mL

21.2784 mL

5 mM

0.4256 mL

2.1278 mL

4.2557 mL

10 mM

0.2128 mL

1.0639 mL

2.1278 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (10.64 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (10.64 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (10.64 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Räisänen-Sokolowski A, et al. Mycophenolate mofetil (MMF, RS-61443) inhibits inflammation and smooth muscle cell proliferation in rat aortic allografts. Transpl Immunol. 1995;3(4):342-351. [Content Brief]

[2]. Hebebrand D, et al. Limb xenotransplantation using FK506 and RS61443 immunosuppression. J Reconstr Microsurg. 1998;14(3):191-194. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1278 mL	10.6392 mL	21.2784 mL	53.1960 mL
	5 mM	0.4256 mL	2.1278 mL	4.2557 mL	10.6392 mL
	10 mM	0.2128 mL	1.0639 mL	2.1278 mL	5.3196 mL
	15 mM	0.1419 mL	0.7093 mL	1.4186 mL	3.5464 mL
	20 mM	0.1064 mL	0.5320 mL	1.0639 mL	2.6598 mL
	25 mM	0.0851 mL	0.4256 mL	0.8511 mL	2.1278 mL
	30 mM	0.0709 mL	0.3546 mL	0.7093 mL	1.7732 mL
	40 mM	0.0532 mL	0.2660 mL	0.5320 mL	1.3299 mL
	50 mM	0.0426 mL	0.2128 mL	0.4256 mL	1.0639 mL
	60 mM	0.0355 mL	0.1773 mL	0.3546 mL	0.8866 mL
	80 mM	0.0266 mL	0.1330 mL	0.2660 mL	0.6650 mL
	100 mM	0.0213 mL	0.1064 mL	0.2128 mL	0.5320 mL

Mycophenolate mofetil hydrochloride

13 Cited Publications

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Mycophenolate mofetil hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Mycophenolate mofetil hydrochloride

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (13)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Mycophenolate mofetil hydrochloride Related Classifications

HY-B0199A Related Classifications