Mycophenolate Mofetil (Synonyms: RS 61443; TM-MMF)

Name: Mycophenolate Mofetil
Brand: MedChemExpress
SKU: HY-B0199
Rating: 5.0 (12 reviews)

Cat. No.: HY-B0199 Purity: 99.83%: Data Sheet
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Mycophenolate mofetil (RS 61443) is the morpholinoethylester proagent of Mycophenolic acid. Mycophenolate mofetil inhibits de novo purine synthesis via the inhibition of inosine monophosphate dehydrogenase (IMPDH). Mycophenolate mofetil shows selective lymphocyte antiproliferative effects involve both T and B cells, preventing antibody formation.

For research use only. We do not sell to patients.

CAS No. : 128794-94-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Mycophenolate Mofetil:

Mycophenolate Mofetil-d₄ In-stock
Mycophenolate mofetil hydrochloride In-stock
Mycophenolate Mofetil (Standard) In-stock

12 Publications Citing Use of MCE Mycophenolate Mofetil

Flow Cytometry

Cell Imaging/Staining

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

In Vivo Efficacy Study

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: Hepatobiliary Surg Nutr. 2023 Dec 1;12(6):835-853.; To investigate the effects of immunosuppressants on PBMCs, peripheral blood mononuclear cells from healthy volunteers were exposed to Mycophenolate Mofetil (15 µg/mL, 3 days).

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: Hepatobiliary Surg Nutr. 2023 Dec 1;12(6):835-853.; The cytolytic activity of NK cells on K562 and HL60 cells. NK cells sorted by MACS were exposed to TAC, CSA, Mycophenolate Mofetil (15 µg/mL, 3 days), EVE, SIR, and CS and cultured for 3 d as effector cells.

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]; The morphology of Mycophenolate Mofetil-LA@DSPE-PEG and Mycophenolate Mofetil-LA@PEG-PLA characterized by transmission electron microscopy (TEM).

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]; The diameters and zeta potentials of Mycophenolate Mofetil-LA@DSPE-PEG and Mycophenolate Mofetil-LA@PEG-PLA characterized by Dynamic light scattering (DLS).

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]; Cell viabilities of Huh7 cells, SUN‐449 cells, LM3 cells and Hep1‐6 cells treated with free Mycophenolate Mofetil, Mycophenolate Mofetil‐LA@DSPE‐PEG and Mycophenolate Mofetil‐LA@PEG‐PLA for 48 h.

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]; Cell cycles determined by flow cytometry. Cells were treated with free Mycophenolate Mofetil (2 µg/mL) or Mycophenolate Mofetil-LA NPs (at 2 µg/mL Mycophenolate Mofetil-equivalent dose) for 48 h.

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]; Colony Formation of Hep1-6 cells, SUN-449 cells and Huh7 cells. Hep1-6 cells, SUN-449 cells and Huh7 cells were treated with drugs at 0.5, 2 and 4 µg/mL (Mycophenolate Mofetil-equivalent concentration), respectively, for 96 h.

: Mycophenolate Mofetil purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Apr;25(7):3511-3523. [Abstract]; Images of Hep1‐6 tumours after treatment with drugs at 20 mg/kg (Mycophenolate Mofetil‐equivalent concentration). Mice were orally administrated with free MMF (20 mg/kg) or intravenously injected with MMF‐LA NPs (at 20 mg/kg MMF‐equivalent dose) every other day for four times.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.05 μM Compound: Mycophenolate	Growth inhibition of human A549 cells Growth inhibition of human A549 cells	[PMID: 28814374]
B-cell	IC₅₀	3.2 μM Compound: MMF	Immunosuppressive activity in BALB/c mouse B cells assessed as reduction in LPS-induced cell proliferation Immunosuppressive activity in BALB/c mouse B cells assessed as reduction in LPS-induced cell proliferation	[PMID: 32323537]
BHK-21	CC₅₀	3.43 μM Compound: 63	cytotoxicity against golden hamster BHK-21 cells incubated for 72 hrs by MTT assay cytotoxicity against golden hamster BHK-21 cells incubated for 72 hrs by MTT assay	[PMID: 32845145]
LLC-MK2	CC₅₀	3.01 μM Compound: 63	Cytotoxicity against Rhesus macaque LLC-MK2 cells incubated for 72 hrs by MTT assay Cytotoxicity against Rhesus macaque LLC-MK2 cells incubated for 72 hrs by MTT assay	[PMID: 32845145]
Splenocyte	EC₅₀	0.48 μM Compound: Mycophenolate mofetil	Immunosuppressive activity in BALB/c mouse splenocytes assessed as reduction in LPS-induced mouse B-lymphocytes proliferation incubated for 48 hrs by MTT assay Immunosuppressive activity in BALB/c mouse splenocytes assessed as reduction in LPS-induced mouse B-lymphocytes proliferation incubated for 48 hrs by MTT assay	[PMID: 36596229]
Splenocyte	IC₅₀	0.21 μg/mL Compound: Mycophenolate mofetilf	Immunosuppressive activity in BALB/c mouse splenocytes assessed as reduction in ConA-induced mouse B-lymphocyte proliferation Immunosuppressive activity in BALB/c mouse splenocytes assessed as reduction in ConA-induced mouse B-lymphocyte proliferation	[PMID: 38441877]
Splenocyte	IC₅₀	0.5 μM Compound: Mycophenolate mofetil	Immunosuppressive activity in BALB/c mouse splenocytes assessed as inhibition of LPS-stimulated B-cell proliferation incubated for 48 hrs by MTT assay Immunosuppressive activity in BALB/c mouse splenocytes assessed as inhibition of LPS-stimulated B-cell proliferation incubated for 48 hrs by MTT assay	[PMID: 33544615]
Splenocyte	IC₅₀	1 μM Compound: Mycophenolate mofetil	Immunosuppressive activity in mouse splenocyte assessed as reduction in LPS-induced mouse B-cell proliferation Immunosuppressive activity in mouse splenocyte assessed as reduction in LPS-induced mouse B-cell proliferation	[PMID: 38128907]

In Vitro

The inosine monophosphate dehydrogenase is an important enzyme in the de novo synthesis of guanosine nucleotides in T and B lymphocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

433.49

Formula

C₂₃H₃₁NO₇

CAS No.

128794-94-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCN1CCOCC1)CC/C(C)=C/CC2=C(O)C3=C(COC3=O)C(C)=C2OC

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (230.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M NaOH : 33.33 mg/mL (76.89 mM; ultrasonic and adjust pH to 12 with 1M NaOH)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3069 mL	11.5343 mL	23.0686 mL
5 mM	0.4614 mL	2.3069 mL	4.6137 mL
10 mM	0.2307 mL	1.1534 mL	2.3069 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (245 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Simmons WD, et al. Preliminary risk-benefit assessment of mycophenolate mofetil in transplant rejection. Drug Saf. 1997;17(2):75-92. [Content Brief]

[2]. Fulton B, et al. Mycophenolate mofetil. A review of its pharmacodynamic and pharmacokinetic properties and clinical efficacy in renal transplantation. Drugs. 1996;51(2):278-298. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

1M NaOH / DMSO	1 mM	2.3069 mL	11.5343 mL	23.0686 mL	57.6715 mL
	5 mM	0.4614 mL	2.3069 mL	4.6137 mL	11.5343 mL
	10 mM	0.2307 mL	1.1534 mL	2.3069 mL	5.7671 mL
	15 mM	0.1538 mL	0.7690 mL	1.5379 mL	3.8448 mL
	20 mM	0.1153 mL	0.5767 mL	1.1534 mL	2.8836 mL
	25 mM	0.0923 mL	0.4614 mL	0.9227 mL	2.3069 mL
	30 mM	0.0769 mL	0.3845 mL	0.7690 mL	1.9224 mL
	40 mM	0.0577 mL	0.2884 mL	0.5767 mL	1.4418 mL
	50 mM	0.0461 mL	0.2307 mL	0.4614 mL	1.1534 mL
	60 mM	0.0384 mL	0.1922 mL	0.3845 mL	0.9612 mL
DMSO	80 mM	0.0288 mL	0.1442 mL	0.2884 mL	0.7209 mL
DMSO	100 mM	0.0231 mL	0.1153 mL	0.2307 mL	0.5767 mL

Mycophenolate Mofetil Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mycophenolate Mofetil128794-94-5RS 61443 TM-MMFRS61443RS-61443Drug MetaboliteApoptosisEndogenous MetaboliteBacterialInhibitorinhibitorinhibit

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Mycophenolate Mofetil (Synonyms: RS 61443; TM-MMF)

Customer Review

Other Forms of Mycophenolate Mofetil:

Mycophenolate Mofetil Related Antibodies

12 Publications Citing Use of MCE Mycophenolate Mofetil

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•Related Screening Libraries:

•Related Small Molecules:

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View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Mycophenolate Mofetil Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Mycophenolate Mofetil Related Classifications

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