1. Apoptosis
  2. Apoptosis
  3. Azathioprine

Azathioprine  (Synonyms: BW 57-322)

Cat. No.: HY-B0256 Purity: 99.98%
COA Handling Instructions

Azathioprine (BW 57-322) is an orally active immunosuppressive agent. Azathioprine can be converted in vivo to the active metabolite 6-mercaptopurine (6-MP). Azathioprine has myelosuppressive effects and induces apoptosis.

For research use only. We do not sell to patients.

Azathioprine Chemical Structure

Azathioprine Chemical Structure

CAS No. : 446-86-6

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10 mM * 1 mL in DMSO USD 61 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

Azathioprine (BW 57-322) is an orally active immunosuppressive agent. Azathioprine can be converted in vivo to the active metabolite 6-mercaptopurine (6-MP). Azathioprine has myelosuppressive effects and induces apoptosis[1][3].

In Vitro

Azathioprine (0-50 μM, 48 hours) can induce severe intracellular GSH depletion with relevant concentrations in both primary rat and human hepatocytes[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Rat hepatocytes, Human hepatocytes
Concentration: 0-50 μM
Incubation Time: 24-48 hours
Result: Showed the decrease in cell viability and intracellular GSH levels in rat hepatocytes as low concentration of 0.5 μM but no significant decrease in cell viability at concentrations below 50 μM as well as GSH depletion was obviously noted at a concentration as low as 1 μM in human hepatocytes.
In Vivo

Azathioprine (oral gavage, 25-400 mg/kg, everyday, 10days) can affect bone marrow cells, red blood cells, and peripheral blood cytokines and other related parameters in a dose-dependent manner, and can induce apoptosis in female CD-1 mice and ICR mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Outbred female CD-1 mice, Female ICR mice[3]
Dosage: 25-400 mg/kg
Administration: Oral gavage; everyday; 10days
Result: Induced a decrease in erythrocyte-related parameters as well as leukocyte-related parameters in a dose-dependent manner.
Induced 52.4%, 35.4%, 17.9%, 16.1% and 15.2% reduction in bone marrow cells at concentrations of 40, 60, 80, 100 and 120 mg/kg, respectively while fms-like tyrosine kinase-3(FLT-3) ligand (FL)-related cytokines were increased.
Clinical Trial
Molecular Weight

277.26

Formula

C9H7N7O2S

CAS No.
SMILES

CN1C(SC2=C3N=CNC3=NC=N2)=C([N+]([O-])=O)N=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (180.34 mM; Need ultrasonic)

H2O : 0.1 mg/mL (0.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6067 mL 18.0336 mL 36.0672 mL
5 mM 0.7213 mL 3.6067 mL 7.2134 mL
10 mM 0.3607 mL 1.8034 mL 3.6067 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (7.50 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (7.50 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (7.50 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.98%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Azathioprine
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