Art. -Nr.: HY-W006957

N6-(2-Hydroxyethyl)adenosine

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N6-(2-Hydroxyethyl)adenosine is a purine nucleoside analog. N6-(2-Hydroxyethyl)adenosine inhibits NF-κB/Smad signaling pathway, exhibits anti-hyperglycemia, antioxidant, antitumor and anti-inflammatory and insecticidal activities. N6-(2-Hydroxyethyl)adenosine is orally active.

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Reinheit: 99.78%
CAS. Nr.: 4338-48-1
Formel: C₁₂H₁₇N₅O₅
Molecular Weight:311.30

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

N6-(2-Hydroxyethyl)adenosine Chemical Structure

CAS. Nr.: 4338-48-1

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

Alle NF-κB Isoform-spezifische Produkte anzeigen :

Alle TGF-beta/Smad Isoform-spezifische Produkte anzeigen :

Smad1
Smad3

In Vitro

N6-(2-Hydroxyethyl)adenosine (5-20 µg/mL, 24 h) reduces the expression of LPS (HY0D1056)-induced TNF-α and IL-1β, increases the IL-10 secretion in RAW 264.7 cell^[3].
N6-(2-Hydroxyethyl)adenosine (5-20 µg/mL, 24 h) reduces TGF-β1-induced expression of collagen I, α-SMA and fibronectin in NRK-49F cell, exhibits anti-fibrosis effect^[3].
N6-(2-Hydroxyethyl)adenosine (0-300 µM, 24-72 h) induces the generation of ROS, decreases mitochondrial membrane potential, exhibits cytotoxicity in cancer cells SGC-7901 and AGS, and induces apoptosis and autophagy^[4].
N6-(2-Hydroxyethyl)adenosine (1 mg/mL, 60 h) exhibits insecticidal activity against Plutella xylostella^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

SGC-7901 and AGS
Concentration:

0-300 µM
Incubation Time:

72 h
Result:

Inhibited cell viability.

Apoptosis Analysis^[4]

Cell Line:

SGC-7901 and AGS
Concentration:

0-150 µM
Incubation Time:

48 h
Result:

Induced apoptosis.

Real Time qPCR^[3]

Cell Line:

RAW 264.7 and NRK-49F
Concentration:

5-20 µg/mL
Incubation Time:

24 h
Result:

Inhibited the expression of TNF-α, IL-1β, collagen I, α-SMA and fibronectin.
Increased the expression of IL-10.

In Vivo

N6-(2-Hydroxyethyl)adenosine (20-40 mg/kg, ip, once daily for 6 weeks) reduces the blood glucose level and diabetes-induced kidney damage, increases the activities of antioxidant enzymes (such as SOD, catalase CAT and GSH) in renal tissue, reduces the level of malondialdehyde (MDA), exhibits anti-inflammatory and antioxidant activities, and ameliorates Alloxan (HY-W017227)-induced diabetes in rats models^[2].
N6-(2-Hydroxyethyl)adenosine (75-150 mg/kg, po, once daily for 19 days) exhibits antitumor effect in SGC-7901 xenograft mouse models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Alloxan-induced diabetes in rats models^[2]
Dosage:

20-40 mg/kg
Administration:

ip, once daily for 6 weeks
Result:

Reduced the levels of blood glucose, serum creatinine (Scr), blood urea nitrogen (BUN) and urine protein.
Reduced the levels of TNF-α, IL-1β, IL-6, TGF-β1 and NF-κB in kidney tissue.
Alleviated the damage of glomeruli and renal tubules.

Animal Model:

SGC-7901 xenograft mouse models^[4]
Dosage:

75-150 mg/kg
Administration:

po, once daily for 19 days
Result:

Reduced tumor volume and weight.

Chemical Information

CAS. Nr. 4338-48-1
Appearance Solid
Molecular Weight 311.30
Formel C₁₂H₁₇N₅O₅
Color White to off-white
SMILES

OCCNC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CO)O3
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

Clin Transl Med

HSF4/COIL complex-dependent R-loop mediates ultraviolet-induced inflammatory skin injury. [Abstract]

2023 Jul;13(7):e1336. PMID: 37461263

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (321.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2124 mL

16.0618 mL

32.1236 mL

5 mM

0.6425 mL

3.2124 mL

6.4247 mL

10 mM

0.3212 mL

1.6062 mL

3.2124 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Man S, et al. Potential and promising anticancer drugs from adenosine and its analogs. Drug Discov Today. 2021 Jun;26(6):1490-1500. [Content Brief]

[2]. Wang X, et al., N6 -(2-hydroxyethyl)-adenosine from Cordyceps cicadae protects against diabetic kidney disease via alleviation of oxidative stress and inflammation. J Food Biochem. 2019 Feb;43(2):e12727. [Content Brief]

[3]. Zheng R, et al., N6-(2-Hydroxyethyl) Adenosine From Cordyceps cicadae Ameliorates Renal Interstitial Fibrosis and Prevents Inflammation via TGF-β1/Smad and NF-κB Signaling Pathway. Front Physiol. 2018 Sep 4;9:1229. [Content Brief]

[4]. Xie H, et al., N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo. Int J Mol Sci. 2020 Aug 13;21(16):5815. [Content Brief]

[5]. Fang M, et al., N6-(2-Hydroxyethyl)-Adenosine Exhibits Insecticidal Activity against Plutella xylostella via Adenosine Receptors. PLoS One. 2016 Sep 26;11(9):e0162859. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2124 mL	16.0618 mL	32.1236 mL	80.3089 mL
	5 mM	0.6425 mL	3.2124 mL	6.4247 mL	16.0618 mL
	10 mM	0.3212 mL	1.6062 mL	3.2124 mL	8.0309 mL
	15 mM	0.2142 mL	1.0708 mL	2.1416 mL	5.3539 mL
	20 mM	0.1606 mL	0.8031 mL	1.6062 mL	4.0154 mL
	25 mM	0.1285 mL	0.6425 mL	1.2849 mL	3.2124 mL
	30 mM	0.1071 mL	0.5354 mL	1.0708 mL	2.6770 mL
	40 mM	0.0803 mL	0.4015 mL	0.8031 mL	2.0077 mL
	50 mM	0.0642 mL	0.3212 mL	0.6425 mL	1.6062 mL
	60 mM	0.0535 mL	0.2677 mL	0.5354 mL	1.3385 mL
	80 mM	0.0402 mL	0.2008 mL	0.4015 mL	1.0039 mL
	100 mM	0.0321 mL	0.1606 mL	0.3212 mL	0.8031 mL

N6-(2-Hydroxyethyl)adenosine

1 Cited Publications

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N6-(2-Hydroxyethyl)adenosine

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

TGF-beta/Smad Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Anti-diabetic Compound Library

Nucleotide Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Mitochondria-Targeted Compound Library

Transcription Factor-Targeted Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Cuproptosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

MCE Kinase Hinge Binder Fragment Library

Publications Citing Use of MedChemExpress (MCE) N6-(2-Hydroxyethyl)adenosine

Alle NF-κB Isoform-spezifische Produkte anzeigen

Alle TGF-beta/Smad Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

N6-(2-Hydroxyethyl)adenosine Related Classifications

Art. -Nr.: HY-W006957