2. Protein Tyrosine Kinase/RTK Autophagy
  3. Bcr-Abl Autophagy
  4. Nilotinib hydrochloride

Nilotinib hydrochloride  (Synonyms: AMN107 hydrochloride)

Cat. No.: HY-10159B Purity: 99.17%
COA
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Nilotinib (AMN107) hydrochloride is an orally active Bcr-Abl tyrosine kinase inhibitor with antineoplastic activity and can be used in studies of chronic myelogenous leukaemia.

For research use only. We do not sell to patients.

CAS No. : 923288-95-3

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Based on 48 publication(s) in Google Scholar

Other Forms of Nilotinib hydrochloride:

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Top Publications Citing Use of Products

48 Publications Citing Use of MCE Nilotinib hydrochloride

Cell Migration/Invasion Assay
Cell Proliferation/Viability Assay
Bio/Physico-chemical Assay
IHC

    Nilotinib hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 Jun;31(6):165.  [Abstract]

    CCK-8 assays were used to assess the IC50 of Nilotinib (0–20 μM) in JHH7, HepG2, Li7, Huh7, Hep3B, and SNU182 liver cancer cell lines over 48 h.

    Nilotinib hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2019;20(6):877-885.  [Abstract]

    Nilotinib (0.1 μM; 15 days). The anti-colony formation effect of CHMFL-ABL-039 against K562, KU812, MEG-01, and BaF3-BCR-ABL-V299L cells was observed.

    Nilotinib hydrochloride purchased from MedChemExpress. Usage Cited in: Oncotarget. 2018 Apr 24;9(31):22158-22183.  [Abstract]

    Primary tumors are dissected at the end of experiment and subjected to immunohistochemistry. Representative images of primary tumor sections stained with anti-PCNA (proliferation), anti-cleaved caspase 3 (apoptosis), and anti-CD31 (angiogenesis).

    Nilotinib hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Med. 2016 Nov;5(11):3223-3234.  [Abstract]

    Direct effect of dasatinib on cytotoxic lymphocytes. Mononuclear cells obtained from peripheral blood before taking dasatinib were incubated with dimethylsulfoxide (DMSO) vehicle, 100 nmol/L or 300 nmol/L dasatinib, 2 μmol/L imatinib, or 2 μmol/L Nilotinib, for 1 h.

    Nilotinib hydrochloride purchased from MedChemExpress. Usage Cited in: Leuk Lymphoma. 2015;56(8):2416-23.  [Abstract]

    Cell cycle analysis of HT93A cells by flow cytometry. Cells are treated with 100 nM Nilotinib and 50 ng/mL G-CSF alone or in combination for 48 h before the cell cycle is analyzed. Nilotinib treatment increases the fraction of cells in G0/G1 phase and decreased the S+G2/M-phase fraction.

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    Description

    Nilotinib (AMN107) hydrochloride is an orally active Bcr-Abl tyrosine kinase inhibitor with antineoplastic activity and can be used in studies of chronic myelogenous leukaemia[1][2][3].
    In Vitro

    Nilotinib hydrochloride, selective Abl inhibitor, is designed to interact with the ATP-binding site of BCR-ABL with a higher affinity than imatinib while being significantly more potent compared with imatinib (IC50<30 nM), also maintains activity against most of the BCR-ABL point mutants that confer Imatinib resistance[1].
    Nilotinib hydrochloride demonstrates significant antitumor efficacy against GIST xenograft lines and imatinib-resistant GIST cell lines which parent cell lines GK1C and GK3C shows imatinib sensitivity with IC50 of 4.59±0.97 μM and 11.15±1.48 μM, respectively, imatinib-resistant cell lines GK1C-IR and GK3C-IR shows Imatinib resistance with IC50 values of 11.74±0.17 μM (P<0.001) and 41.37±1.07 μM (P<0.001), respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nilotinib hydrochloride Related Antibodies
    In Vivo

    Nilotinib hydrochloride (oral gavage, 40 mg/kg, daily, 4 weeks) shows equivalent or higher antitumor effects in BALB/cSLc-nu/nu mice with GIST xenograft[2].
    Nilotinib hydrochloride has a significant healing effect on the macroscopic and microscopic pathologic scores and ensures considerable mucosal healing in the indomethacin-induced enterocolitis rat model while decreases the PDGFR α and β levels and apoptotic scores in the colon[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/cSLc-nu/nu mice with GIST xenograft (GK1X, GK2X and GK3X)[2]
    Dosage: 40 mg/kg
    Administration: Oral gavage; daily; 4 weeks
    Result: Inhibited tumor growth by 69.6% in GK1X, 85.3% in GK2X and 47.5% in GK3X xenograft line.
    Molecular Weight

    565.98

    Formula

    C28H23ClF3N7O
    CAS No.
    Appearance

    Solid

    SMILES

    O=C(NC1=CC(C(F)(F)F)=CC(N2C=C(C)N=C2)=C1)C3=CC=C(C)C(NC4=NC=CC(C5=CC=CN=C5)=N4)=C3.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 125 mg/mL (220.86 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7668 mL 8.8342 mL 17.6685 mL
    5 mM 0.3534 mL 1.7668 mL 3.5337 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.17%

    COA (245 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7668 mL 8.8342 mL 17.6685 mL 44.1712 mL
    5 mM 0.3534 mL 1.7668 mL 3.5337 mL 8.8342 mL
    10 mM 0.1767 mL 0.8834 mL 1.7668 mL 4.4171 mL
    15 mM 0.1178 mL 0.5889 mL 1.1779 mL 2.9447 mL
    20 mM 0.0883 mL 0.4417 mL 0.8834 mL 2.2086 mL
    25 mM 0.0707 mL 0.3534 mL 0.7067 mL 1.7668 mL
    30 mM 0.0589 mL 0.2945 mL 0.5889 mL 1.4724 mL
    40 mM 0.0442 mL 0.2209 mL 0.4417 mL 1.1043 mL
    50 mM 0.0353 mL 0.1767 mL 0.3534 mL 0.8834 mL
    60 mM 0.0294 mL 0.1472 mL 0.2945 mL 0.7362 mL
    80 mM 0.0221 mL 0.1104 mL 0.2209 mL 0.5521 mL
    100 mM 0.0177 mL 0.0883 mL 0.1767 mL 0.4417 mL
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nilotinib923288-95-3AMN107AMN 107AMN-107Bcr-AblAutophagytyrosine kinaseantitumorInhibitorinhibitorinhibit

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